BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,649	A→C	8.8%	Y260S (TAC→TCC) ‡	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,649	0	A	C	8.8%	12116.8 / 272.1	4843	Y260S (TAC→TCC) ‡	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (3128/1181); new base C (25/498); total (3160/1683)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4971 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  3:272657/1‑139 (MQ=255)

    aGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  3:179859/1‑138 (MQ=255)

         cACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGGATCGTAGTTATCTACACGACggg                                                                                                                                  >  3:31373/1‑139 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                  >  2:90096/1‑137 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                          >  2:105870/1‑139 (MQ=255)

                  ggccGGCCATTCCGGTTGGCGTGTTTATTCCATAAAAAGCTGCAGCCGGGGCGCGTGGGTGGCGCGGTTTCATTGCTGCACGGGGGCA‑ATATGTTACCCCCCCCCGTATCTTATTTATCAACACGACGGGGAGTCAGGc                                                                                                                         <  2:28642/136‑1 (MQ=255)

                  gcgcGGCCCTGCGGGGGGGCTGGTTTTTTGCTCATAAAGGTGGCGCGGGGGTGCGGGGGGGTCGCGTTATAATCGCACCAGGGGGGCC‑AGTTGGAACCCCCCCCCGTAAGTTATTTATCAACACGACGGGGAGTCAGGc                                                                                                                         <  4:153050/136‑1 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  8:148174/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  5:196880/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  8:54697/1‑138 (MQ=255)

                          cTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGTTGTTAAGCCCTCCCGTATCTTAGTTATATCCACGACGGGGAGTCAGGCAACTATgg                                                                                                                 <  8:291505/139‑1 (MQ=255)

                           ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                                                >  3:119151/1‑139 (MQ=255)

                                  tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGCGGCC‑AGATGGTAACCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACg                                                                                                          >  6:239195/1‑138 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  5:32524/1‑139 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  2:379517/1‑139 (MQ=255)

                                      tggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  6:209924/1‑137 (MQ=255)

                                           ttttGCTTATAATTCGGGCGCCGGTGCGGGGGGGGCGCGCTGAATCATGGCAGCACGGGGCCC‑TGATGGTACGCCCGCCCGTCTCTTAGTTCTCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACa                                                                                                 <  8:184920/136‑1 (MQ=255)

                                              tGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGa                                                                                                   >  7:182197/1‑133 (MQ=255)

                                                ctaaaaaaTCGGGCGCGGGTGAGGGTGGGTCGCGGGGTATCATGGCACCACGGGGGCC‑AGATGGTCACCCCTCCCTTATCGTAGTTAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCg                                                                                            <  6:405248/133‑1 (MQ=255)

                                                 ttaTAATTCTCGAGCGGGTGAGCGGGGGCGTCGCGTTATCATTGCTCCTGTGGGGCC‑AGATGCTTACCCGTCCCGTTTGGATGATATATCCACGACGGGGAGTCAGGAAACTATGGATGAACGAAATAGACAGATAGCt                                                                                          <  8:267610/137‑1 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  7:66965/1‑137 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  8:216026/1‑137 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  7:30717/1‑137 (MQ=255)

                                                          tGGCGCCGGTGCGCGGGGGTCGCGCGGTATCATTGCACCACTGGGGCC‑AGATGGTAAGCCCCCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGt                                                                                 <  7:343578/139‑1 (MQ=255)

                                                                cGGTGAGCGTGGGGCTGTCTGTATCATTGCACCACTGGGCCC‑CGATGGTAAGCCCACCCGTATCGTAGTACTCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  6:369800/139‑1 (MQ=255)

                                                                 ggTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAc                                                                          >  3:880/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  5:14630/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  1:217459/1‑139 (MQ=255)

                                                                      gCGTGGGGCTCGGGGTATAATTGCCGAACTGGGGCA‑GGTTGTTAAGCCCTCCCGTATGTTTTTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  5:108558/139‑1 (MQ=255)

                                                                       ggggggTCGCGCGTTATCATTGCACCACGGGGCCC‑AGATGGTACGCCCCCCCTTAGCGTTGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTa                                                                    <  8:415443/136‑1 (MQ=255)

                                                                         tGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGATTAAGGCAACTATGAATGAACGAAATAGACAGATCGATCAGATAGGTGCCTCACTGAtt                                                                     >  6:161189/1‑136 (MQ=255)

                                                                         tGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGATCGAAATAGACAGATCGCTGAGATAGGTGCCGCACTGATTAAg                                                                  >  1:346616/1‑139 (MQ=255)

                                                                         tGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTacg                                                                  >  4:250845/1‑137 (MQ=255)

                                                                                cggggTATCATCGCCCCACTGGGGCC‑CTTAGGTACGCCCTCCCTTATCGTTGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAGAGGTGCCTCACTGATTAAGCATTGGt                                                           <  5:46545/136‑1 (MQ=255)

                                                                                cgcgGTATCATTCCACCAGGGGGCCC‑AGATGGTACGCCCCCCGGTATCTTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           <  8:35109/139‑1 (MQ=255)

                                                                                 gcgGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTTCCGCACTGATGAAGCATTGGt                                                           >  8:380033/1‑138 (MQ=255)

                                                                                 gcgGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           >  2:411262/1‑138 (MQ=255)

                                                                                            gcagcaCTGGGGCC‑ATATGGTAAGCCCTCCGGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                <  3:414027/138‑1 (MQ=255)

                                                                                               gcaCTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  4:341845/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  3:228169/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  5:357541/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  5:384930/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  5:26235/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  4:121463/1‑139 (MQ=255)

                                                                                                 gcgggggCC‑AGATGGTAAGCCCGCCGGTAGCGTAGATAACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  2:348913/136‑1 (MQ=255)

                                                                                                 acgggggCC‑CGATGGTACGCACGCCCGTTGCTTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  7:234161/136‑1 (MQ=255)

                                                                                                 acgggggCC‑ATATGTTAAGCCCTCCCGTAGCGTATTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  7:44362/136‑1 (MQ=255)

                                                                                                  cTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  4:77315/1‑138 (MQ=255)

                                                                                                  acggggCCAAGATGGCACCCCCTCCCGAATCGTAGTTACCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  1:275734/137‑1 (MQ=255)

                                                                                                   tGGGTCC‑AGTTTGTAAGCCCTCCGGTATCTTATTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:294058/139‑1 (MQ=255)

                                                                                                   tGGGGCC‑CTATGGTACGCCCTCCCTTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  6:304693/139‑1 (MQ=255)

                                                                                                   tGGGGCC‑ATATGTTACCCCCACCGTTATGTTATTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  3:381112/139‑1 (MQ=255)

                                                                                                   tGGGGCC‑AGAGGGTACGCCCCCCCGTAGGGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  1:7854/139‑1 (MQ=255)

                                                                                                   gggggCC‑AGATGGTAACCCCCCCTGTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  8:326102/138‑1 (MQ=255)

                                                                                                   gggggCC‑AGATGGAACCCCCGACCGTATCTTATTAACCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  6:339534/138‑1 (MQ=255)

                                                                                                   gggggCC‑AGATGGAAACCCCGCCCGTCTCGTAGTTAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  6:90784/138‑1 (MQ=255)

                                                                                                   cggggCC‑TGATGTAAACCCCCCCCGTATCTTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:4123/138‑1 (MQ=255)

                                                                                                     ggggC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGATATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGATTAc                                       >  2:219348/1‑138 (MQ=255)

                                                                                                     gggCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCCGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  5:171483/1‑139 (MQ=255)

                                                                                                     gggCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             >  7:125036/1‑68 (MQ=255)

                                                                                                     gggCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  7:367563/137‑1 (MQ=255)

                                                                                                     gggCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  2:139059/1‑139 (MQ=255)

                                                                                                         actgATGGTAAGCCCTCCCTTTTCTTAGATAGCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCat                                   <  4:404440/136‑1 (MQ=255)

                                                                                                           ataTGTTAAGCCTCCCCTTATCTTATTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  8:244214/137‑1 (MQ=255)

                                                                                                           aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           >  4:187337/1‑97 (MQ=255)

                                                                                                           aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  7:278983/1‑130 (MQ=255)

                                                                                                           aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                  >  8:73693/1‑138 (MQ=255)

                                                                                                           aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                  >  1:101105/1‑138 (MQ=255)

                                                                                                           aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                  >  5:223416/1‑138 (MQ=255)

                                                                                                                        tCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAGGCATTGGTAACTGTCAGACCACGTTTACTCATATATCCTTTAgattg                    >  1:171321/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          <  2:25324/68‑1 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          <  6:109835/68‑1 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTATCTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  6:5679/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCCGACCAAGTTTACTCATATATAGTTTAgattga                   >  1:307568/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  1:249840/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  3:347681/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTCAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  3:151598/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCAACACGACGGGTAGTCAGGCAAATATGCATGAACGAAATAGCCAGCACGCAGAGATAGGTGCATCACTGATTAAGCAATGGAAACTGTCAGACCAAGATTAATCATATATACTTTAgatct                    >  7:400235/1‑136 (MQ=255)

                                                                                                                          ccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattgat                  >  4:375917/1‑139 (MQ=255)

                                                                                                                               tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  8:123473/1‑138 (MQ=255)

                                                                                                                                cGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaaaa            >  7:253068/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           >  1:37867/1‑90 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  7:94564/116‑1 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  7:209870/1‑139 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  1:55004/1‑138 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGAGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  6:13522/1‑138 (MQ=255)

                                                                                                                                      tATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAt      >  7:160591/1‑139 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           >  1:406216/1‑69 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  4:430335/69‑1 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  6:181193/104‑1 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  4:303021/1‑104 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  6:271429/1‑104 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  2:57566/1‑139 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  2:181565/1‑139 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  1:131141/1‑139 (MQ=255)

                                                                                                                                         cTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   >  3:205071/1‑139 (MQ=255)

                                                                                                                                          tACCCGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACCGATCGCTGAGATAGGTGCCTCACTGATTAAGCAGTGGTAACTGTCAGCCCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   >  3:52821/1‑138 (MQ=255)

                                                                                                                                          tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:79293/101‑1 (MQ=255)

                                                                                                                                          tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   <  2:312190/138‑1 (MQ=255)

                                                                                                                                          tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   >  4:213561/1‑138 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC‑AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`