BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	5,464	T→G	5.4%	intergenic (+733/‑424)	ampR → / → lacZalpha	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	5,464	0	T	G	5.4%	20756.2 / 184.0	9380	intergenic (+733/‑424)	ampR/lacZalpha	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (4384/4205); new base G (306/392); total (4763/4617)
Fisher's exact test for biased strand distribution p-value = 2.88e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9426 total aligned reads displayed.

AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT  >  Exported/5326‑5601

    cGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                        >  6:215941/1‑138 (MQ=255)

      gACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTc                                                                                                                                          <  6:73530/134‑1 (MQ=255)

      gACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAg                                                                                                                                     >  7:297733/1‑139 (MQ=255)

       aCAGGTACCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                    <  3:137542/139‑1 (MQ=255)

             acccGGTACGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                              <  7:385538/137‑1 (MQ=255)

              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCgg                                                                                                                             >  6:189985/1‑139 (MQ=255)

                   tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGcc                                                                                                                         >  2:27360/1‑138 (MQ=255)

                   tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGcc                                                                                                                         >  3:108687/1‑138 (MQ=255)

                   tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCt                                                                                                                        <  6:111315/139‑1 (MQ=255)

                     aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                      >  8:215285/1‑139 (MQ=255)

                      gCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATg                                                                                                                     >  4:339635/1‑139 (MQ=255)

                      gCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATg                                                                                                                     >  7:69404/1‑139 (MQ=255)

                                  gAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCCCCTCTGCCTTGAGCGTCGATTTTTTTGTTGCTGGTCCGGGGGGCGGGGCCGAAGGAAAAACGCCAg                                                                                                         >  7:156087/1‑139 (MQ=255)

                                       ggAGAGCCCACGTGGGCGCTTCCCGGGGGAAACGCGTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTCACTTGCGCGTCGATTTTTGTGATGCGGGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcg                                                                                                    <  8:159109/139‑1 (MQ=255)

                                          gagCGCACGTGGGAGCTGCCAGGGGTAAACGCCTGGTATCTTTATAGTCCTGGCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTGGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                 <  5:11636/139‑1 (MQ=255)

                                          gagCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTTTGTTGGTCGTCAGGGGGGCGGGGCCGATGGCAAAACGCCCACCACGCgg                                                                                                 >  1:356936/1‑139 (MQ=255)

                                           agCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACCCGGc                                                                                                >  3:215586/1‑139 (MQ=255)

                                            gcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGCTTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGcc                                                                                               >  7:353222/1‑139 (MQ=255)

                                            gcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                              <  2:121913/108‑1 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTGTATGCGAGTCAGGGCGGCCGACCCGATGGAAAAACGCCACCAAAGCgggca                                                                                              >  5:52265/1‑136 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                    >  6:363414/1‑101 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgcg                                                                                                  <  7:93682/135‑1 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                              >  2:312761/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                              >  5:191136/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                              >  5:345904/1‑139 (MQ=255)

                                               cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGGGGAGCCTATGGAAAAACCCCAGCCACGCGGCCgtt                                                                                            >  3:244202/1‑139 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGAGTTTTGTGATGGTCGGCAGGGGGGCGGCGCCATTGGAAAAACGCCAGCCACGCGGCCGTTTTa                                                                                         >  7:213776/1‑139 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                         >  1:288050/1‑139 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                         <  4:30429/139‑1 (MQ=255)

                                                   aGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                        <  1:100033/139‑1 (MQ=255)

                                                    gggAGCTTCCTGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACg                                                                                       <  5:315482/139‑1 (MQ=255)

                                                         ctccccgggggAAACGCCTGGTATCTTTATAGCCCAGGCGGGTTGCCCCACCTCTGACTTGCGCGGCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                                  <  3:282132/133‑1 (MQ=255)

                                                                   gAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttg                                                                        <  1:228594/139‑1 (MQ=255)

                                                                    aaccGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTGGATTTTTCTGCTGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCCACGCGGCCTTTTTACGGTTCctggcctttttc                                                                       >  6:138236/4‑137 (MQ=255)

                                                                    aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGCCTTGAGCGGCGATTTTTGTGGTGCTCGTCAGTCGGGCGGGGCCGTTGGAAAAACGCCCACAACGCGGCCTGTTTACGGTTActgggctttttc                                                                       >  6:389665/1‑137 (MQ=255)

                                                                     aaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgct                                                                      >  2:38040/1‑139 (MQ=255)

                                                                          cTGGTATCTTTATTGTCCTGTCGGGTTTCGCCAACTCTGACTTGTGCGTCGTTTTTTGTGATGCTGGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGGCTTTTTACGGTTCctggccttttgctggcct                                                                 <  5:84294/139‑1 (MQ=255)

                                                                           tGGTATCTTTTTTGTCCTGTGGGGTTTCGCCACCTCTGACTTGAGCGGCGATTTTTGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                                <  1:154501/139‑1 (MQ=255)

                                                                             gTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttt                                                               <  7:322347/138‑1 (MQ=255)

                                                                              tATCTTTATAGTCCTGGCGGGGTTCGCCCCTGCTGACTGGTGCTTCGATTTTGGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                             <  1:424164/139‑1 (MQ=255)

                                                                                  tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGTTGGTCGTGAGGGGGGCGGAGCCTATTGTAAAACGCCAGCAACGCGGCCCGTTTAAGGGTCcaggccgtttgcggggcttttgg                                                            >  4:267699/1‑135 (MQ=255)

                                                                                  tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTca                                                         >  1:125514/1‑139 (MQ=255)

                                                                                  tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTca                                                         >  8:123412/1‑139 (MQ=255)

                                                                                    tataGTCCTGTCGGTTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTGGTCTGGGGGGCGGAGCATATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                        >  2:200589‑M2/1‑137 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGTTGCTGGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                        >  1:320843‑M2/1‑137 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTc                                                          <  8:339695/136‑1 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAca                                                       <  1:67522‑M2/139‑3 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAca                                                       <  1:386850‑M2/139‑3 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAca                                                       <  5:288293‑M2/139‑3 (MQ=255)

                                                                                        gTCCTGTCGGTTTTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGTAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                   <  6:42384/139‑1 (MQ=255)

                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAcatgt                                                    <  2:74847‑M2/138‑6 (MQ=255)

                                                                                        gTCCTGTCGGGTTGCGCCACCTCTGACTTGAGCGGCGATTTTTGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                   <  7:186232/139‑1 (MQ=255)

                                                                                        gTCCTGTCGGGATTCGCCACCTCTGACGTGAGCGTCGATTTTTGGCATGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAcatgt                                                    >  8:375279‑M2/1‑133 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGTTGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACCCCACCAACGCGGCCGTTTTACGGTGCCGGGCCTTTTGGTGGCCTTTTGGTCTcatggtgtt                                                <  2:242572‑M2/139‑10 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGGTGCTCGTCAGGGGGGCGGGGCCTATGGAAAAACCCCAGCAACGCGGCCGTTTTACGGGTGCTTGCCTTTTGCGGGGCGTTTTCTCAcatgttgtt                                                <  4:86934‑M2/139‑10 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                                  >  4:132724/1‑105 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                             <  4:195011/126‑1 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCt                                                 <  4:429632/138‑1 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGTGCCGATTGAAAAACGCCAGCAACGCGGCCTTATTACGGGTCCTGGCCTTTTGGTGGCCGTTTGCTGAAAAGTTGttt                                               >  4:166130/1‑139 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAcatgt                                                    >  6:300553‑M2/1‑129 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCCGACTTGAGCGGCGATTTTTGTGATGGTCGTCAGGGGGGCGGCGCCTATGGAAAAAAGCCAGCAACGCCGGCGTTTTACGGGTCCTGGCCTTTTGGTGGCCTTTTGCTCACATGTTCttt                                               >  5:253804/1‑139 (MQ=255)

                                                                                             gTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                   >  3:341422/1‑134 (MQ=255)

                                                                                             gTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                              <  2:48361/139‑1 (MQ=255)

                                                                                             gTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGAAACGCGGCCTTTTTACGGTTCCTGTCCTTTTGCTGGCCTTTTGCTAAAATGTTCTTTc                                              >  7:242880/1‑139 (MQ=255)

                                                                                              tCGTGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                              <  7:413882/138‑1 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTACTGTCCTTTTGCTGGCCTTTTGGTACCATGTTCTTTcc                                             >  1:92917/1‑139 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                                  <  8:192638/102‑1 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTAACATGTTCTTTcc                                             >  7:101828/1‑139 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGGCGATTTTTGTGATGGCCGTCAGGGGGGCGGAGCCAATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                              <  6:326095/138‑1 (MQ=255)

                                                                                                  ggttCGCCACCTCTGAGTTGCGCGTCGATTTTTGGGTTGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCg                                         <  8:48308/137‑1 (MQ=255)

                                                                                                   tttCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                 <  3:314171/82‑1 (MQ=255)

                                                                                                      cGCCACCTCTGACTTGAGCGTGGTTTTTTGTGATGCTCGTCAGGGGTGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                      <  4:398592/138‑1 (MQ=255)

                                                                                                      cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                      <  8:408653/138‑1 (MQ=255)

                                                                                                       gCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCTGGGGGGCGGAGCATATGGAAAAACGCCAGCAGCGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCTCATGTTCTTTCCTGCGTTATc                                    <  2:346226/139‑1 (MQ=255)

                                                                                                          aCCTCTGACTTGTGGGTCGATTTTTGTGATGGTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATcccc                                 <  4:222169/139‑1 (MQ=255)

                                                                                                          aCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATcccc                                 <  4:372359/139‑1 (MQ=255)

                                                                                                          aCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATcccc                                 <  3:190728/139‑1 (MQ=255)

                                                                                                                 ccTTGCGCGTCGATTTTTGTGATGCGCGCCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCt                          <  2:154269/138‑1 (MQ=255)

                                                                                                                 cattGAGCGTCGATTTTTGTTAGGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                            <  5:16801/135‑1 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                            <  1:19602/137‑1 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                            <  3:71206/137‑1 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                 >  3:165014/1‑63 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                              >  7:129768/1‑114 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGa                     >  7:50479/1‑139 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  3:396455/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  8:74018/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  3:99748/139‑1 (MQ=255)

                                                                                                                         gTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                  <  7:269694/139‑1 (MQ=255)

                                                                                                                           cGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTACGGGCCTTTTGCTGGCCTTTTGCTCCCAAGTTCTTTACTGCGTTATACCCAGATTCTGTGGATAAcc                >  8:275321/1‑139 (MQ=255)

                                                                                                                                tttGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTAt            <  6:164243/138‑1 (MQ=255)

                                                                                                                                  tgtgATGCTGGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  6:26079/138‑1 (MQ=255)

                                                                                                                                  tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  5:219175/138‑1 (MQ=255)

                                                                                                                                  tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  3:422518/138‑1 (MQ=255)

                                                                                                                                  tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  5:412498/138‑1 (MQ=255)

                                                                                                                                  tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCGCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  8:307836/138‑1 (MQ=255)

                                                                                                                                   gtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTAc         <  8:245758/138‑1 (MQ=255)

                                                                                                                                    tgATGCGCGTCAGGGGGGCGGAGCCTATGTAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCATTTTGCGGGCCTTTTGATCACATGTTCTTTCCTGCGTTATCCCCTGATTATGTGGATAACCGTATTAcc        <  5:376263/138‑1 (MQ=255)

                                                                                                                                      aTGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAc                 <  1:260307/127‑1 (MQ=255)

                                                                                                                                        gCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                  <  3:372886/124‑1 (MQ=255)

                                                                                                                                         cTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCt    <  5:142376/137‑1 (MQ=255)

AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT  >  Exported/5326‑5601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`