BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,031	A→C	6.7%	A48A (GCA→GCC)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,031	0	A	C	6.7%	22796.2 / 261.8	8525	A48A (GCA→GCC)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (3442/4321); new base C (698/25); total (4168/4357)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9794 total aligned reads displayed.

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169

 aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTTGGAAAACCCAGGC‑GGTACCCCAAATAATCGGCGTTCAGcac                                                                                                                                           >  8:191152/1‑139 (MQ=255)

 aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGcac                                                                                                                                           >  3:101509/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCCCAt                                                                                                                                         >  7:302216/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                                         >  5:337071/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGC‑GTTACCCCACGTAATCGCCTTGCAGCACAt                                                                                                                                         >  3:346382/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACATGGTAAACCCAGGC‑GGTACCCAACATAATCCCCGTGCACCACAt                                                                                                                                         >  4:40593/1‑139 (MQ=255)

    aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                                        <  1:367650/139‑1 (MQ=255)

        cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTGTTACACCGTCGTGGCTGGGGAAACCCAGGC‑GTTACCCCACTTATTAGCCTTGCCGCACATccccc                                                                                                                                    >  2:64778/1‑139 (MQ=255)

         gCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGC‑GGTACCCAACTTAATCGCCTAGCAGCACATcccccg                                                                                                                                   >  8:401057/1‑138 (MQ=255)

             aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGC‑GTTACCCAACGTAATCGCCGTTCAGCCCCTCCCCCATTCg                                                                                                                               >  8:336336/1‑139 (MQ=255)

             aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTAACACGTCGTGACTGGGATAACCCAGGC‑GGTACCCAACATAATACCCATGCACCACCACCCCCATTCg                                                                                                                               >  5:142125/1‑139 (MQ=255)

                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                          <  1:100119/138‑1 (MQ=255)

                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                          <  5:306591/138‑1 (MQ=255)

                       ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATAGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                                     >  7:219074/1‑139 (MQ=255)

                        cTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCAGGC‑GTTACCCAACTTAATCGCCGTGCAGCACCTCCCCCATTAGCCCGCTGGCg                                                                                                                     >  2:102674/1‑138 (MQ=255)

                        cTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAAACCCGGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGt                                                                                                                    <  6:29407/139‑1 (MQ=255)

                         tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGt                                                                                                                    >  7:362693/1‑138 (MQ=255)

                              gTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                                        <  7:69076/113‑1 (MQ=255)

                                 gaccatAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCTTTTAACAACGACGGGACTGGGAAAACCCGGGC‑GATACCCAACTTAATCGCCTTGCCGCACATCCCCCTTGCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  4:45552/134‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGCAAGGGAAAACCCAGGCGGTTACCC‑ACGTAAAAGCCATGCCCCACCACCCCCATTCGCCCCCCGGGGTGATAGCgca                                                                                                           >  6:257789/1‑137 (MQ=255)

                                 acagctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  1:24082/135‑1 (MQ=255)

                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GGTTCCCCACGTAATAGCCTTTCAGCACCTCCCCCTTTCGCCCGCTGGCGTTATAGCGTag                                                                                                          >  2:132570/1‑139 (MQ=255)

                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGCCGGGGAAAACCCAGGC‑GGTACCCCACTTAATCGCCTTGCAGCCCCTCCCCCATTCGCCAGCCGGCGTAATAGCGaa                                                                                                           >  4:246709/1‑138 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTGGTGACTGGGAAACCCCTGGC‑GTTACCCAACGTAATATCCTTGCAGCACCTCCCCCGTTCGCCAGCGGGGGTTATAGCGCAGAGGCCCGCAcc                                                                                               >  6:114145/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGC‑GTTTCCCAACTTAATCGCCTTGCAGCCCCACCCCCCTTCGCCCGCTGGCGGAATAGCGAAGAGGCCCCCAcc                                                                                               >  3:374566/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                               >  2:247647/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACGTAATCGCCGTGCCGCACCTCCCCCTTTCGCCAGCGGGCGTTATTGCGAAGAGGCCCGcccc                                                                                               >  2:309993/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGC‑GTTACCCAACTTAATCGCCTTGGAGCACATCCCCCTATCCCCAGCTGGCGTTATATGGAAGAGGCCCCCAcc                                                                                               >  5:411768/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACCTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATAGCCTTGCAGCACGGCACCCGTTCGCCCGCAGGCGTAAAAGCGAAGAGGCCCCCAcc                                                                                               >  7:150203/1‑139 (MQ=255)

                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCACAATAATCGCCTTGCAGCCACTACCCCGATCGCCCGCAGGCCTAATAGCGAAGCGGCCCCCACCg                                                                                              >  4:343201/1‑139 (MQ=255)

                                               cccGGGTCCCGAGCTCGAATTCACTGGCCGGCGTTTTACACCGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCGCACCGa                                                                                             <  2:378315/139‑1 (MQ=255)

                                                ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGACTGGGAAACCCCTGGC‑GGTACCCAACTTAATCGCCGTGCAGCACATCCCCCTTTCGCCCGCTGGGGGAATATCGTAGAGGCCCGCCCCGAt                                                                                            >  5:362061/1‑139 (MQ=255)

                                                       ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                                                     <  7:333695/139‑1 (MQ=255)

                                                       ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGCAAACCCTGGC‑GTTTCCCAACATTATAGCCTTGCCGCCCCTACCCCTTTCGCCACCTGGCGTAAAAGCGAAGACGCCCCCCCCCAACGcccat                                                                                     >  3:25351/1‑137 (MQ=255)

                                                        cGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑CTTACCCAACATAATCGCCTTGCAGCACCTCCCCCATTCGCCAGCAGGCGTAATAGCGAAGAGGCCCGCCCCGAACCCCCATc                                                                                    >  7:246112/1‑139 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                  <  3:1587/139‑1 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                  >  5:208529/1‑139 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                  >  5:187271/1‑139 (MQ=255)

                                                               gAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATTGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACa                                                                              <  4:81247/138‑1 (MQ=255)

                                                                     aCTGGCCGTGGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTTCCCAACTTAATCGCCTTGCAGCACATCCGCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  6:412006/139‑1 (MQ=255)

                                                                     aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  1:173227/139‑1 (MQ=255)

                                                                     aCTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  7:271725/139‑1 (MQ=255)

                                                                         gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                                                    <  8:225084/138‑1 (MQ=255)

                                                                         gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                                                    <  8:48324/138‑1 (MQ=255)

                                                                         gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                                                    <  1:206961/138‑1 (MQ=255)

                                                                         gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATAGCCTTGCAGCACATCCCCCATTAGCCAGCTGGCGTAATAGCGCAGAGGCCCCCCACGAACGCCCATACCAACACTTGCGCAGc                                                                    >  8:72331/1‑138 (MQ=255)

                                                                          ccGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGCTCGCCCTTCCCAACAGTTGCGCCGCCt                                                                  >  8:126676/1‑139 (MQ=255)

                                                                                     cAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                                                       <  4:207141/139‑1 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                  <  6:218089/107‑1 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                  >  5:169063/1‑139 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                  >  1:303661/1‑139 (MQ=255)

                                                                                          ccgggACTGGCAAAACCCTGGC‑GATACCCAACTAAATCGCCTTGCCGCACACCCCCCTTGCGCCCGCGGGCGTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACCGTTGCGCAGCCTGAATGGCGAATGgcgc                                                  <  3:218909/135‑1 (MQ=255)

                                                                                             tGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTg                                               <  7:120974/139‑1 (MQ=255)

                                                                                             tGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGACGCCCGCACCGATCGcc                                                                                        >  1:40334/1‑98 (MQ=255)

                                                                                             tGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCACCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGCCCCGCACCGATAGCCCTTCCCAACAGTAGCGCCCCCTGAATGGCGAATGGCGCCt                                                >  3:187952/1‑138 (MQ=255)

                                                                                               aCTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCACCAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGAt                                             <  3:429689/139‑1 (MQ=255)

                                                                                                  gggAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTAGCGCCAGCGGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                          <  5:59227/139‑1 (MQ=255)

                                                                                                   ggAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                           <  7:397087/137‑1 (MQ=255)

                                                                                                   ggAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCgg                                         >  2:224807/1‑139 (MQ=255)

                                                                                                   ggAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                          <  2:42714/138‑1 (MQ=255)

                                                                                                    gAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGATGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCAGTATGGCGATAGGCGCGTGCTGCGGt                                        >  8:280812/1‑139 (MQ=255)

                                                                                                    gAAAACCCTGGC‑GTTACCCAAATTAAACGCCTTGCAGCACCTCCCCCGTTCGCCAGCAGGCGTAATAGCGAAGAGGCCCGCCCCGATCCCCCGTCCCAACAGTTGCCGCCCCTGAATGGAGAAAGGCCGCTGAAGcag                                         >  2:173407/1‑136 (MQ=255)

                                                                                                         cccTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                   <  3:404102/139‑1 (MQ=255)

                                                                                                             ggC‑GTTACCCAACTTAATCGCCTTGCAGCACATcccc                                                                                                                                     >  6:191445/1‑37 (MQ=255)

                                                                                                             ggC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                          <  4:17462/48‑1 (MQ=255)

                                                                                                             ggC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           >  8:304932/1‑63 (MQ=255)

                                                                                                             ggC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                               >  6:90613/1‑139 (MQ=255)

                                                                                                             ggC‑GTTACCCAACTTAATCGCCTTCCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCCAACGCCCAACCCAACATTTGCGCAGCCTGAATGGCGAATGGCTCCTGAAGCGGTATTGtctac                               >  3:74664/1‑137 (MQ=255)

                                                                                                                    aCCCAACTTACTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          <  1:33608/138‑1 (MQ=255)

                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                                     <  6:170816/47‑1 (MQ=255)

                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           >  5:109956/1‑57 (MQ=255)

                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGccc                                                                                       >  6:385304/1‑77 (MQ=255)

                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGccc                                                                                       <  5:385304/77‑1 (MQ=255)

                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          >  1:48348/1‑138 (MQ=255)

                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          >  8:370124/1‑138 (MQ=255)

                                                                                                                     cccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGCTATTTTCTCCTTACGCa                        >  3:318487/1‑139 (MQ=255)

                                                                                                                      ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                       <  7:186098/139‑1 (MQ=255)

                                                                                                                      ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                       <  1:69646/139‑1 (MQ=255)

                                                                                                                         aCTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtg                    <  7:91408/139‑1 (MQ=255)

                                                                                                                         aCTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtg                    >  3:133613/1‑139 (MQ=255)

                                                                                                                          cTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                   >  5:272039/1‑139 (MQ=255)

                                                                                                                          cTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                   >  2:274707/1‑139 (MQ=255)

                                                                                                                          cTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCCGCCTGATGCGGTATTTTCTACTTACGCATCtgt                   >  4:145366/1‑139 (MQ=255)

                                                                                                                             aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTTGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCTCAGCCTGAATGGCGAATGGCGCCTGATTCGGTATTTTCTCCTTACGCATAGGTGCg                >  1:273460/1‑139 (MQ=255)

                                                                                                                             aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  1:136782/137‑1 (MQ=255)

                                                                                                                             aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  5:90118/137‑1 (MQ=255)

                                                                                                                              aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCAGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                <  7:426834/138‑1 (MQ=255)

                                                                                                                               tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                            >  4:281896/1‑125 (MQ=255)

                                                                                                                               tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCgg               >  3:287009/1‑138 (MQ=255)

                                                                                                                                  ccTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCCGCCTGCATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAtt           >  6:58554/1‑139 (MQ=255)

                                                                                                                                    ttGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                           >  4:315423/1‑105 (MQ=255)

                                                                                                                                      gcagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  4:37341/139‑1 (MQ=255)

                                                                                                                                      gcagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTca        <  6:327784/138‑1 (MQ=255)

                                                                                                                                      gcagcaCAGCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCGGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  6:143161/139‑1 (MQ=255)

                                                                                                                                      gcagcaCACCCCCCTTTCGCCAGCTGGCGTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  5:242347/139‑1 (MQ=255)

                                                                                                                                       cagccCATCCCCCTTTCGCCAGCTGTCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  4:111145/138‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTTGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCATCCCAACAGTTGCGCAGCCTGAATGCCGAATGGCGCCTGATGCGGGTTTTTCTCCTTACGCATCTGGGCGGTATTTcaca      <  6:358204/139‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGATGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  2:340133/138‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  8:15706/138‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  8:24983/138‑1 (MQ=255)

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`