BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,047	A→C	5.4%	S54R (AGC→CGC)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,047	0	A	C	5.4%	28209.6 / 272.4	10834	S54R (AGC→CGC)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (3568/6423); new base C (654/127); total (4230/6604)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 10865 total aligned reads displayed.

TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCA  >  Exported/5910‑6185

  cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                           <  4:128280/138‑1 (MQ=255)

  cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                           <  3:300683/138‑1 (MQ=255)

  cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                           <  3:117596/138‑1 (MQ=255)

  cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCt                                                                                                                                          >  6:151677/1‑139 (MQ=255)

    tGCCTGCAGGTCGACTCTAGAGGATCCCCGTGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTgg                                                                                                                                        <  2:123406/139‑1 (MQ=255)

      ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAACACCCAGGCGTTTCCCCACTTAATAGCCGTGCCGCCCCTCCCCCGTTCGCCCGCGGGC‑g                                                                                                                                     >  5:7074/1‑139 (MQ=255)

        tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGCAAACCCTGGCGTTACCCCACGTAATCGCCTTGCAGCCCCGCCCCCGTTCCCCCGCGGGC‑gg                                                                                                                                    >  1:270889/1‑137 (MQ=255)

        tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑Gt                                                                                                                                    >  8:71465/1‑138 (MQ=255)

         gCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTGACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTaa                                                                                                                                  <  2:264962/139‑1 (MQ=255)

          cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTaa                                                                                                                                  <  5:153197/138‑1 (MQ=255)

                gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGaa                                                                                                                           <  7:353933/139‑1 (MQ=255)

                gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGaa                                                                                                                           <  5:98926/139‑1 (MQ=255)

                gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGaa                                                                                                                           <  8:183528/139‑1 (MQ=255)

                gACTCTAGAGGATCCCCGGGGACCGATCTCGAATTCAATGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCTCCTTGCAGCACATCCCCCTTTCGCAAGCTTGC‑GTAATAGCGaa                                                                                                                           <  8:277754/139‑1 (MQ=255)

                 aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGGACTTGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCACCCTTACGCCAGCTGGC‑GTAATAGCGAag                                                                                                                          <  8:33152/139‑1 (MQ=255)

                  ctctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGACGGGGAAAACCCTGGCGTTGCCCCACTTAATCGCCTTGCCGCACATCCCCCTTTCGCCCGCTGGC‑GGTATAGCGaa                                                                                                                           >  4:354345/1‑137 (MQ=255)

                       gagGATCCCCGGGTACCGAGCTCGAAATCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCACATCCCCGTTTCGCCAGCTGGC‑TTAATAGCGAAGAgg                                                                                                                       <  3:64783/136‑1 (MQ=255)

                        cggATCCCCGGGTACCGATCTCGAATTAACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATGGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGAGACGAGGCCCg                                                                                                                   <  2:33163/138‑1 (MQ=255)

                        agGATCCCCGGGTACCGAGCTCGAATTCAGTGGCGGTCGTTTTACTACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCGTTGCAGCACATCCCCGTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCg                                                                                                                   <  7:172315/139‑1 (MQ=255)

                            tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGACAGGGAAAACCCAGGCGTTTCCCCACTTAATAGCCGAGCAGCACAAACCCCGTTCGCCAGCTGGC‑GTAATATCGAAAAGGCCCCCAcc                                                                                                               >  6:367036/1‑139 (MQ=255)

                            tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACCCTGGCGTTACCCAACGTAATCGCCTTGCCGCCCATCCCCCATTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCAcc                                                                                                               >  6:75654/1‑139 (MQ=255)

                            tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCAcc                                                                                                               >  7:293062/1‑139 (MQ=255)

                            tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCCCCTTAATCGCCTTGCAGCACCTCCCCCGTTCGCCCGCGGGC‑GTAATATCGAAGAGGCCCGcccc                                                                                                               >  3:91658/1‑139 (MQ=255)

                            tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACGGGGCAAACCCGGGCGTTTCCCAACGTAATCGCCGTGCAGCCCATCCCCCTTTCGCCCGCGGGC‑GGAAGAGCGTAGCGGCCCGcccc                                                                                                               >  4:225845/1‑139 (MQ=255)

                            tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTAGTGGCTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCAcc                                                                                                               >  4:296106/1‑139 (MQ=255)

                             ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTAAACCCTGGCGGTACCCCACATAATCGCCGTGCCGCACCTCCCCCGTTCCCCCCCGGGC‑GGAATAGCGAAGAGGCCCGCCCCg                                                                                                              >  1:238433/1‑139 (MQ=255)

                             ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATCGCCTTGCAGGACCTCCCCCTTTCGCCAGCTGGC‑GGAATAGCGAAGAGGCCCgggcgc                                                                                                              >  8:302565/1‑134 (MQ=255)

                               ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCCACGTGGTGACTGGGTAAACCCGGGCGTTACCCCCCTTTATCGGCGTGCCGCACAGACCCCGTTCGCCCCCTGGCGGTAAT‑GCGAAGGGGCCCGCCCCGag                                                                                                            >  2:29780/1‑138 (MQ=255)

                               ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGaa                                                                                                            >  5:427741/1‑138 (MQ=255)

                                   gTACCGAGCTCGAATGCACTGGCCGTCGTTTTACAAGGTCGTGAGTGGGAAAACCGTGGCGTTACCAAACTTAATCGCCTTGCAGCACATCCCCGTTTCGCCAGCTGGC‑GTATTAGCGATGAGGCCCGCACCGATCGcc                                                                                                        <  6:235920/139‑1 (MQ=255)

                                    tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGccc                                                                                                       <  6:307055/139‑1 (MQ=255)

                                         aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTTCCCCACTTAATCGCCTTGCCGCACCTCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCCCCGATCGCCCTTccc                                                                                                  >  1:42725/1‑139 (MQ=255)

                                         aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGC‑GGAATAGCGAAGAGGCCCGCACCGCGCGCCCTTccc                                                                                                  >  8:118212/1‑139 (MQ=255)

                                         aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                                  >  5:179337/1‑139 (MQ=255)

                                              gAATTCACTGTCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAACCAAATAAATCGCCTTGAAGAACATCCCCCTTTAGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACa                                                                                              <  4:387017/138‑1 (MQ=255)

                                                    aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                                       <  1:331121/139‑1 (MQ=255)

                                                    aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                                       <  5:255898/139‑1 (MQ=255)

                                                     cTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTTAAAGCGAAGAGGCCCGCCCCGATCGCCCGTCCCCCCAGTTgcgcc                                                                                      >  3:393617/1‑138 (MQ=255)

                                                        gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                                                                    <  1:63738/138‑1 (MQ=255)

                                                           gtcgtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCAAGCTGGC‑GTAATAGCGAAGAGGCCAGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                                                                <  7:383239/139‑1 (MQ=255)

                                                           gtcgtTTTACAACGTCGGGACGGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                                                                <  2:202497/139‑1 (MQ=255)

                                                                       cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCCGCCTgaatggcgaatggc                                                                    >  1:16878/1‑139 (MQ=255)

                                                                         cgtggACTGGGAAAACCGTGGCGTTCCCCAAGTTAAACGCGTTGCGGCCCAACCCCCTTTCCCCAGCTGGC‑GTAATAGCGAAGAGCCCCGCCCCCATCGCCGTGCCCACCGGTTGCGCCGCCGgaatgggcaatggccc                                                                  >  2:363967/5‑137 (MQ=255)

                                                                            tGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTaa                                                                                                                                  <  4:91135/72‑1 (MQ=255)

                                                                                  tcaaacCCCTGGCTTAACCCAACTAAATCGCCTTGCAGCACATCCCCATTTCGCCAGCTGGC‑TTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                                          <  3:273797/136‑1 (MQ=255)

                                                                                  ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCCTCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCGGAATGGCGCATGGCGCCTGATGCgg                                                         >  7:85009/1‑139 (MQ=255)

                                                                                  ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCgg                                                         >  5:227153/1‑139 (MQ=255)

                                                                                  ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                                           <  6:426975/137‑1 (MQ=255)

                                                                                  ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                                           <  6:202820/137‑1 (MQ=255)

                                                                                  ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                                          <  2:54055/138‑1 (MQ=255)

                                                                                   gAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                                  <  1:133525/129‑1 (MQ=255)

                                                                                        cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                                   <  2:83365/139‑1 (MQ=255)

                                                                                        cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                                   <  7:233199/139‑1 (MQ=255)

                                                                                          cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                                       <  8:398881/101‑1 (MQ=255)

                                                                                          cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                                 <  2:23794/139‑1 (MQ=255)

                                                                                          cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                                 <  1:299667/139‑1 (MQ=255)

                                                                                          cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCACATTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                                 <  7:57518/139‑1 (MQ=255)

                                                                                            ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                                               >  3:144829/1‑139 (MQ=255)

                                                                                               gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                                            <  6:179480/139‑1 (MQ=255)

                                                                                                  taccAACTTAATCGCCATGCAGCACATCCCCCTTTCGCCAGCAGGC‑GTAATAGCGAAAAGGCCCCCACCGAACGCCCCTCCCAACAGTATCACAGCCTGAAAGGCCCAAGGCGCCAGCTGCGGTATATTCTCCATACg                                          >  4:65230/3‑138 (MQ=255)

                                                                                                  aCCCACCTTAATCGCCTTGCAGCACATCCCCCATTCCCCAGCTGCC‑GTAAAAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGATGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                                          <  4:158506/138‑1 (MQ=255)

                                                                                                  aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGaa                                                                                                                           <  3:360413/57‑1 (MQ=255)

                                                                                                  aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                                          <  6:272590/138‑1 (MQ=255)

                                                                                                  aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAATAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                                          >  1:94132/1‑138 (MQ=255)

                                                                                                   cccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGcc                                                                 <  7:104768/114‑1 (MQ=255)

                                                                                                   cccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCa                                        >  5:320788/1‑139 (MQ=255)

                                                                                                    ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGC‑GTAATCGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                                       <  5:28731/139‑1 (MQ=255)

                                                                                                       aCTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                                            <  8:43395/131‑1 (MQ=255)

                                                                                                           aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                                  <  7:136664/137‑1 (MQ=255)

                                                                                                            aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                                <  8:147449/138‑1 (MQ=255)

                                                                                                            aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                                <  3:172373/138‑1 (MQ=255)

                                                                                                             tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                                          <  3:207919/111‑1 (MQ=255)

                                                                                                                  ttGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt                          >  6:124438/1‑138 (MQ=255)

                                                                                                                    gcagcaCATCCCCCTTTCGCCAGCTGGC‑GTAATAGCTAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTca                        <  4:326420/138‑1 (MQ=255)

                                                                                                                    gcagcaCATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTca                        <  8:36628/138‑1 (MQ=255)

                                                                                                                    gcagcaCATCCCCCTATCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACACATCTGTGCGGTATTTcac                       <  4:19081/139‑1 (MQ=255)

                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  5:198893/138‑1 (MQ=255)

                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  2:160176/138‑1 (MQ=255)

                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  5:397817/138‑1 (MQ=255)

                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  7:260575/138‑1 (MQ=255)

                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  8:219074/138‑1 (MQ=255)

                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGC‑GTAAAAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcaca                      <  1:201668/139‑1 (MQ=255)

                                                                                                                     cagcaCATCCCCCTTTCGCCAGCGGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  5:37551/138‑1 (MQ=255)

                                                                                                                         acaTCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGCGGCCCGCACCGCTCGCCCTTCCCAACAGTTGCGCAGCCTGACTGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCCTCTGGGCGGTATTTCACACCGc                  <  6:339050/139‑1 (MQ=255)

                                                                                                                           agcccccTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCat                <  3:240739/137‑1 (MQ=255)

                                                                                                                           aTCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                                               <  6:398415/108‑1 (MQ=255)

                                                                                                                             cccccTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGcc                                                                                                        <  5:123615/49‑1 (MQ=255)

                                                                                                                             cccccTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                                           <  1:10008/94‑1 (MQ=255)

                                                                                                                                 cTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTg          <  1:392579/139‑1 (MQ=255)

                                                                                                                                 cTTTAGCCAGCTGTC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCACAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTTCGCATCTGTGATGTATTTCACACCGCATATGGTg          <  1:359454/139‑1 (MQ=255)

                                                                                                                                  tttCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGc         >  4:263476/1‑139 (MQ=255)

                                                                                                                                    tCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGc         <  4:414246/137‑1 (MQ=255)

                                                                                                                                    tCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGc         <  7:324693/137‑1 (MQ=255)

                                                                                                                                    tCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGc         <  3:419381/137‑1 (MQ=255)

                                                                                                                                       ccAGCTGGC‑GTAATCGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActct    <  3:329565/139‑1 (MQ=255)

                                                                                                                                       ccAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActct    <  8:246112/139‑1 (MQ=255)

                                                                                                                                       ccAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActct    <  2:404032/139‑1 (MQ=255)

                                                                                                                                       ccAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGAGCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActct    <  7:20268/139‑1 (MQ=255)

                                                                                                                                       ccAGCTGCC‑GTAATAGCGTAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGTAGAATGGCGACTGATGATGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActct    <  5:351062/139‑1 (MQ=255)

                                                                                                                                         aGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                                           >  7:411889/1‑82 (MQ=255)

TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC‑GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCA  >  Exported/5910‑6185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`