Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	457,443	A→T	5.4%	I141F (ATT→TTT)	pxpA →	oxoprolinase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	457,443	0	A	T	5.4%	234.7 / 3.8	74	I141F (ATT→TTT)	pxpA	oxoprolinase subunit A
Reads supporting (aligned to +/- strand):  ref base A (43/26);  new base T (2/2);  total (46/28)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

TTCTAAAAGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACGCACTCA  >  BS168_reference_genome/457315‑457581                                                                                                                                 |                                                                                                                                           ttCTAAAAGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAACGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCgg                                                                                                                                  >  8:65610/1‑139 (MQ=255)  tCTAAAAGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGt                                                                                                                                 >  1:147110/1‑139 (MQ=255)  tCTAAAAGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGt                                                                                                                                 >  7:88313/1‑139 (MQ=255)    tAAAAGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     <  8:366892/133‑1 (MQ=255)    tAAAAGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     >  7:366892/1‑133 (MQ=255)      aaaGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGCTTCTTTTCGGTCTTg                                                                                                                             >  6:20173/1‑139 (MQ=255)            gagGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTga                                                                                                                         >  6:25499/1‑137 (MQ=255)             agGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTAGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCTGCCTGATTCTTTTCGGTCTTGCTGagag                                                                                                                      >  4:228051/1‑139 (MQ=255)                 aCTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAgagcgag                                                                                                                  <  1:205450/139‑1 (MQ=255)                          gcagcaCGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGTTTCTTTTCGGt                                                                                                                                 <  4:427010/115‑1 (MQ=255)                          gcagcaCGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGTTTCTTTTCGGt                                                                                                                                 <  4:420444/115‑1 (MQ=255)                          gcagcaCGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGTTTCTTTTCGGt                                                                                                                                 >  3:427010/1‑115 (MQ=255)                          gcagcaCGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGTTTCTTTTCGGt                                                                                                                                 >  3:420444/1‑115 (MQ=255)                             gcaCGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCg                                                                                                      >  1:396660/1‑139 (MQ=255)                                  tCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGgaga                                                                                                 <  8:88313/139‑1 (MQ=255)                                                    tataCAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGAc                                                                               >  7:196161/1‑139 (MQ=255)                                                    tataCAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGAc                                                                               >  7:47410/1‑139 (MQ=255)                                                     ataCAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGAc                                                                               <  7:144345/138‑1 (MQ=255)                                                         aaTATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGCCCGCa                                                                           >  5:322274/1‑138 (MQ=255)                                                           tatGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     >  4:201662/1‑78 (MQ=255)                                                           tatGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     <  3:201662/78‑1 (MQ=255)                                                           tatGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAAt                                                                        >  2:226855/1‑139 (MQ=255)                                                           tatGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAAt                                                                        >  3:154067/1‑139 (MQ=255)                                                             tGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     <  3:33619/76‑1 (MQ=255)                                                             tGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     >  4:33619/1‑76 (MQ=255)                                                              ggcggcAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     <  1:344519/75‑1 (MQ=255)                                                              ggcggcAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCtttt                                                                                                                                     >  2:344519/1‑75 (MQ=255)                                                              ggcggcAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGCCTTCAGACCGCAAATg                                                                       >  8:76854/1‑137 (MQ=255)                                                               gcggcAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAg                                                                    <  2:396660/139‑1 (MQ=255)                                                                cggcAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCTGTCTTGCTGAGAGCGAGCTTGTAAAAGCGTGAGAACGGATCGGACTTCAGACCGCAAATGAAgt                                                                   >  5:237378/1‑139 (MQ=255)                                                                cggcAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAATGCGGGAGAACGGATCGGCCTTCAGACCGCAAATGAAgt                                                                   >  5:312704/1‑139 (MQ=255)                                                                cggcAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATGGGACTTCAGACAGCAAATGAAgt                                                                   >  3:319581/1‑139 (MQ=255)                                                                        gATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCg                                                           <  4:120567/139‑1 (MQ=255)                                                                         aTCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGa                                                          <  8:196161/139‑1 (MQ=255)                                                                         aTCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGa                                                          <  5:25499/139‑1 (MQ=255)                                                                                    tCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCg                                                                                         >  4:427569/1‑97 (MQ=255)                                                                                    tCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCg                                                                                         <  3:427569/97‑1 (MQ=255)                                                                                    tCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCg                                                                             <  5:271356/109‑1 (MQ=255)                                                                                    tCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCg                                                                             >  6:271356/1‑109 (MQ=255)                                                                                              tCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     >  3:96511/1‑139 (MQ=255)                                                                                              tCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     <  6:322274/139‑1 (MQ=255)                                                                                                          tCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCTGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACAcc                         >  3:238436/1‑139 (MQ=255)                                                                                                          tCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACAcc                         >  2:264218/1‑139 (MQ=255)                                                                                                          tCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACAcc                         >  6:422561/1‑139 (MQ=255)                                                                                                              taAGGTTGATCCCGGCGTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACcg                        <  4:238436/136‑1 (MQ=255)                                                                                                                aGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCa                                                                                  >  7:242687/1‑76 (MQ=255)                                                                                                                aGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCa                                                                                  <  8:242687/76‑1 (MQ=255)                                                                                                                aGGGTGATCCCGGCCTGATTCTTTGCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCt                    <  6:19714/138‑1 (MQ=255)                                                                                                                 ggTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGCGCGCGCTTGTAAAAGCGGGAGTACGGCTCGGACTTCAGCCCGCAAATGAAGTGTTCGCGGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTcc                  >  3:239959/1‑139 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     <  2:97041/117‑1 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     >  1:97041/1‑117 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     >  3:70696/1‑117 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     <  4:70696/117‑1 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAg               >  7:69477/1‑139 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAg               >  2:373540/1‑139 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAg               >  8:115803/1‑139 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAg               >  4:150070/1‑139 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAg               >  7:140121/1‑139 (MQ=255)                                                                                                                    tGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAg               >  1:320094/1‑139 (MQ=255)                                                                                                                      aTCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     >  1:144775/1‑115 (MQ=255)                                                                                                                      aTCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACgg                                     <  2:144775/115‑1 (MQ=255)                                                                                                                      aTCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGcc             >  1:90748/1‑139 (MQ=255)                                                                                                                      aTCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGcc             >  6:422041/1‑139 (MQ=255)                                                                                                                      aTCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGcc             >  5:235721/1‑139 (MQ=255)                                                                                                                      aTCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGcc             >  1:270251/1‑139 (MQ=255)                                                                                                                      aTCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGCCACGCTCACACCGCGCTCCCAGcc             >  7:201411/1‑139 (MQ=255)                                                                                                                           ggCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCTGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACg        <  5:292399/139‑1 (MQ=255)                                                                                                                           ggCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACg        <  7:153031/139‑1 (MQ=255)                                                                                                                           ggCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACg        <  6:265648/139‑1 (MQ=255)                                                                                                                           ggCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACg        <  4:96511/139‑1 (MQ=255)                                                                                                                            gCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGa          >  7:167098/1‑136 (MQ=255)                                                                                                                            gCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGa          <  8:167098/136‑1 (MQ=255)                                                                                                                            gCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACGc       >  3:144588/1‑139 (MQ=255)                                                                                                                              cTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACAAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACGCAc     <  3:150070/139‑1 (MQ=255)                                                                                                                                 aTTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGACCGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGCCGCACTCa  >  8:141310/1‑139 (MQ=255)                                                                                                                                 aTTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGCCCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACGCACTCa  >  5:399552/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                           TTCTAAAAGCAGAGGGACTTCACATGCAGCACGTCAAGCCGCATGGCGCTTTATACAATATGGCGGCAGTCGATCAAAAACTGTCGGATGCTATCGCGAAAGCTGTCTATAAGGTTGATCCCGGCCTGATTCTTTTCGGTCTTGCTGAGAGCGAGCTTGTAAAAGCGGGAGAACGGATCGGACTTCAGACCGCAAATGAAGTGTTCGCCGACAGAACATACCAATCGGACGGCACGCTGACACCGCGCTCCCAGCCGGACGCACTCA  >  BS168_reference_genome/457315‑457581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑