| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 648,025 | T→G | 14.5% | F29C (TTT→TGT) | tatCY → | component of the twin‑arginine pre‑protein translocation pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 648,025 | 0 | T | G | 14.5% | 125.6 / 6.9 | 58 | F29C (TTT→TGT) | tatCY | component of the twin‑arginine pre‑protein translocation pathway |
| Reads supporting (aligned to +/- strand): ref base T (16/32); new base G (7/2); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGATTAACGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGACAGACCCGCTTTATGTGTTT > BS168_reference_genome/647889‑648149 | ggATTAACGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTg < 1:238318/139‑1 (MQ=255) gATTAACGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGt > 8:292969/1‑139 (MQ=255) gATTAACGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTATga > 7:53183/1‑138 (MQ=255) ttAACTAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGtag > 2:181380/1‑137 (MQ=255) ttAACGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACATGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCg > 8:303304/1‑139 (MQ=255) ttAACGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGtgtgggg > 8:103578/1‑136 (MQ=255) aaCGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGtt > 3:393271/1‑139 (MQ=255) aaCGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTGTGTCGtt > 4:20490/1‑139 (MQ=255) aaCGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTGTGGCGtg > 5:303745/1‑138 (MQ=255) aCGAGTGCTGAAGAGGAAACACAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGtt < 6:303745/138‑1 (MQ=255) aCGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGtt < 1:232517/138‑1 (MQ=255) ggAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGa < 8:413536/139‑1 (MQ=255) aaaaaaGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑ttt > 8:240953/1‑139 (MQ=255) aaaaaaGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTGTGTGGTTGTTTGTATTTGTGGC‑ttt > 1:135227/1‑139 (MQ=255) agaagcTCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCa < 4:398310/139‑1 (MQ=255) agaagaTCAGTAAGTTAGGATGACATGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCa < 7:303304/139‑1 (MQ=255) agaagaTCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCa < 7:204361/139‑1 (MQ=255) aagaTCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCaaa < 1:315816/139‑1 (MQ=255) agaTCAGTTAGTTAGGATGAGACGAATGTAAGTGTATCTAATGTCGGTGCTGGAGGATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTATCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCaaa < 3:398995/138‑1 (MQ=255) aGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGat < 4:36452/139‑1 (MQ=255) tAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTTTCGTGTTCTTTATTGCTGGA‑TTTTTTTTTGCAAAGCCGATTATtgtg > 1:38471/1‑139 (MQ=255) tAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATtgtg > 6:310689/1‑139 (MQ=255) ggATGACACGAATGACAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTgtgt < 2:328724/138‑1 (MQ=255) ggATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTgtgt < 8:169857/138‑1 (MQ=255) ggATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTGTGTCGTTTTGTTTTTTTGTGGT‑TTTTTTTTATGAAAAACCATTTTTgtgtt > 3:374984/1‑138 (MQ=255) gATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTAt < 3:20490/139‑1 (MQ=255) acacGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑‑TTTTTTTAGCAAAGCCGATTATTGTGTATCTGCa < 2:71714/139‑1 (MQ=255) gaatgaatGTGAATCAAATGTCGCTGATGGAGCATATTGCTGAGCTTCGAATACGGTTGCTGATTTTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGa < 8:194656/139‑1 (MQ=255) aGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTTCTGGT‑TTTTTTTTTGCAAAGCCGATTATTTTGTATCAGCAAGAAACAGAt > 8:2589/1‑139 (MQ=255) aaaTGTCGCTGCTGGAGCTTATTGCTGAGCTTCGAAAACGGTGGCTGAGTGTAGCGCTGGCGTTTGTCGTATTATTTATTGCTGGA‑TTTTTTTTAGCATAGCCGATTATTGTGTATCTGCAAGAAACAGATgaagcgaa < 8:235640/139‑1 (MQ=255) aaaTGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCGGATTATTGTGTATCTGCAAGAAACAGATgaagcgaa < 7:302266/139‑1 (MQ=255) aaaTGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACAGTTGCTGATTGTAGCGCTGGCGTGTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATgaagcgaa < 3:146676/139‑1 (MQ=255) aaTGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATgaagcgaag < 3:347901/139‑1 (MQ=255) aaTGTCGCGGCTGGAGCATATTGCTGAGCTTCGAAAACGGGTGCTGATTGTAGCGCTGGCGTTTGTCGGTTTCTTTATTTCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGTTgaagcgaag < 7:359350/139‑1 (MQ=255) aaTGCCGCTGCTGGAGCATAATGCTGAGCTTCGAAAACGGTTGCTGCATGTAGCGCCTGCGTTTGTCGTTTTCTTTATTGCTGGATTTTTTTTTAGCAAAGCCGATAATTGTGTATCTGCAAGAAACAGATgaagcgaa < 4:391808/139‑1 (MQ=255) cGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGTCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCt < 7:412713/139‑1 (MQ=255) cGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTGTGTCGGGTTGTTTTTTGGTGGA‑GTTTTTTTTGAAAAGCCGATTTTTTTGTTTATGGAAGAAACAGATGAAGCGAagcagcg > 8:173882/1‑138 (MQ=255) gctgctGGAGCATATTGCTGTGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGTAACAGATGAAGCGAAGCAGCt < 5:253920/138‑1 (MQ=255) ctgctgGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTa < 4:393271/139‑1 (MQ=255) ctgctgGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTGTGTCGTTTTGTTTTTTGGTGGT‑TTTTTTTTTGCAAATCCGATTATTTGTTTTAGGCAAGAAAAACATTAAGCCGAGCAGCtt > 4:297629/1‑138 (MQ=255) aGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTaa < 2:127892/139‑1 (MQ=255) aGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTaa < 7:240953/139‑1 (MQ=255) aGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑GTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTaa < 7:2589/139‑1 (MQ=255) tatTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTGGGTTTGTTTTTTTGTGGA‑TTTTTTTTTTCAAAGCCGATTTTTTTGTTTATGCAAGAAACAGATTAATCGAAGCCGCTTAAGCGTAAagcg > 6:368084/1‑139 (MQ=255) tatTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTGTTTTTTTGGGGT‑TTTTTTTTTGGAAAGCCGAATTTTGTGTATATGCAAGAAACAGATGAAGCGCATCAGCATACGCTTTAcgcg > 3:291707/1‑139 (MQ=255) atGCTGAGCTTCGAAAACGGGTGCTGACTGTAGAGCTGGCGTGTGTCGTTTTCTTAATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGtt < 4:194696/138‑1 (MQ=255) gCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGTCGTTTGTCGTTTTCTTTATTTCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGttt < 8:53183/138‑1 (MQ=255) cTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCGGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTaa < 2:135227/139‑1 (MQ=255) aGCTTCGAAAACGGTTGCTGATTGTGGCGCTGGCGTTTGTCGTTTTAATTATTTCAGGA‑TTTTTTTTTCCAAACCCCATTATTTTTTATAAGCAAAAAACAAATAAACCGAACCACCTTACCCTTAACCCGTTTAAca > 6:367671/1‑137 (MQ=255) cTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGa < 5:126487/139‑1 (MQ=255) cGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTGATTTTTTCTTGA‑TTTTTTTTAGCAAAGCCCATTTTTTTTTATGTGCAAGAAACAAATGAAGCGAAGCACCATACCCTTAAACCGTTTAAACtta > 6:126487/1‑134 (MQ=255) cGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGACa < 2:22603/138‑1 (MQ=255) cGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTATTTATTTCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGa > 2:13057/1‑136 (MQ=255) gAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGACAGa < 4:291707/139‑1 (MQ=255) aaaaCGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGGTGGA‑TTTTTTTTTGCAAACCCGATTATTGTGTTTCTTCAAGAAACAGATGTAGCCAAGCAGCTAACGCTTAACGCCGTTAACCTGACAGAc > 7:350754/1‑139 (MQ=255) aaaCGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGACAGAcc < 1:167473/139‑1 (MQ=255) ggTTGCTGATTGTAGCGCTGGCGTGTGTCGTTTTCTTTTTTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGACAGACCCGCt > 5:159352/1‑139 (MQ=255) tGTAGCGCTGGCGTTTGTCGTTTTGTTTATTTGTGGA‑TTTTTTTTAGCAAAGCCGATTATTTTGTATCTGCAAGAAACAGATTAAGCGAAGCCGCTTACCCTTAACGCGTTTAACCTGACAGCCCCGCGTTATGTGttt > 3:238875/1‑139 (MQ=255) tGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGACAGACCCGCTTTATGTGttt > 7:146484/1‑139 (MQ=255) | GGATTAACGAGTGATGAAGAGGAAAAAAAGAAAGAAGATCAGTAAGTTAGGATGACACGAATGAAAGTGAATCAAATGTCGCTGCTGGAGCATATTGCTGAGCTTCGAAAACGGTTGCTGATTGTAGCGCTGGCGTTTGTCGTTTTCTTTATTGCTGGA‑TTTTTTTTAGCAAAGCCGATTATTGTGTATCTGCAAGAAACAGATGAAGCGAAGCAGCTTACGCTTAACGCGTTTAACCTGACAGACCCGCTTTATGTGTTT > BS168_reference_genome/647889‑648149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |