Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,616,570 C→A 15.9% Q102K (CAA→AAA)  ylyA → sporulation‑related RNA polymerase‑binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,616,5700CA15.9% 175.8 / 3.7 77Q102K (CAA→AAA) ylyAsporulation‑related RNA polymerase‑binding factor
Reads supporting (aligned to +/- strand):  ref base C (53/10);  new base A (2/12);  total (55/22)
Fisher's exact test for biased strand distribution p-value = 1.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTCCTTGCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCATA  >  BS168_reference_genome/1616439‑1616708
                                                                                                                                    |                                                                                                                                          
ttCTCCTTGCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAAttt                                                                                                                                       >  1:110952/1‑137 (MQ=255)
 tctcCTTGCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACCGCAAACGATTTTCTTTATTCTGTCCAAttt                                                                                                                                       >  4:144301/1‑136 (MQ=255)
 tctcCTTGCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTgag                                                                                                                                    >  7:365188/1‑139 (MQ=255)
 tctcCTTGCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCAAAtat                                                                                                                                       >  1:74266/1‑134 (MQ=255)
       tGCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTTTCCAATTTGAGaaaaa                                                                                                                               >  1:67542/1‑138 (MQ=255)
        gCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCCTT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGATCCGCAAACGATTTTCGTTATTCTGTGCAATTTGAGaaaaaaa                                                                                                                             >  2:147275/1‑139 (MQ=255)
          aTTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACg                                                                                                                           >  1:20383/1‑139 (MQ=255)
             gTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGCACAGCAAACGATTTTGTTTATTCTGTCCAATTTGAGAAAAAAACGCtt                                                                                                                        >  4:389621/1‑139 (MQ=255)
             gTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGAtt                                                                                                                        >  7:255541/1‑139 (MQ=255)
              tCCAAAATCGAAAACGGCACATTCGTATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGAAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTc                                                                                                                       >  1:26632/1‑139 (MQ=255)
              tCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATA‑CCTCTTGCAAAGCTGGCAGTGCTGCCGCCGGCCCGAACAGCAAACGATTTTCTTTATTCTTTACAATTTGAGAAAAAAACGCTTc                                                                                                                       >  8:269977/1‑139 (MQ=255)
                        aaaaCGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAGAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGa                                                                                                              >  7:130773/1‑138 (MQ=255)
                        aaaaCGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGCCGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAACCGCTTCCGATATGGa                                                                                                              >  8:47552/1‑138 (MQ=255)
                        aaaaCGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTATTTATTCTTTACAATTTGAGAAAAAAACGCTTACGATATGGa                                                                                                              >  5:280105/1‑138 (MQ=255)
                                       gATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGAGTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCg                                                                                               >  5:124714/1‑138 (MQ=255)
                                            tgtgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATg                                                                                         >  1:102513/1‑139 (MQ=255)
                                            tgtgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATg                                                                                         >  1:397038/1‑139 (MQ=255)
                                            tgtgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCGTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATg                                                                                         >  1:377621/1‑139 (MQ=255)
                                            tgtgAAGAAACTGGGGCCCCCATT‑CCTCATGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGAGATTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATg                                                                                         >  3:104605/1‑139 (MQ=255)
                                              tgAAGAAACTGGGGCCCCCATT‑CCTCTTTCAAAGCTGGCAGTGCTGCCGCCGGCCCGAACAGCAAACGATTTTCTTTATTATGTCAATTTTGAGAAAAAAACCCTTCCGATATGGAAATCAACGGATATCGAAAATgga                                                                                       >  5:6102/1‑138 (MQ=255)
                                              tgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGTCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATgg                                                                                        >  1:260047/1‑138 (MQ=255)
                                              tgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGt                                                                                       >  7:155804/1‑139 (MQ=255)
                                              tgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGt                                                                                       >  3:381726/1‑139 (MQ=255)
                                              tgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGt                                                                                       >  7:51802/1‑139 (MQ=255)
                                              tgAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGAATTTCTTTATTCTGTCCATTTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGt                                                                                       >  8:209060/1‑139 (MQ=255)
                                              tgAAGAAACTGGGGCCCCCAAT‑ACAATTGCAAAGAAGGCACTGCAGCCGACGGCCCACACAACAAACGATATTGTTTATTATTTGCAAAATTAGCAAAAAAAGCTAACCAGAAGGGAATAAAACGATATAGAATATgt                                                                                        >  2:217495/1‑137 (MQ=255)
                                              ggaaaaaACGGGGCCCCCAATA‑CATGTTCCAATGGTGGAATTGGTGCCGAGGGCCCTAACACAAAACTATTTTTTTTTTTCTGTCAATTTTTAAAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGt                                                                                       <  2:20383/138‑1 (MQ=255)
                                                aagaaACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGt                                                                                       >  3:270811/1‑137 (MQ=255)
                                                    atgtGGGCCCCACATA‑CCTCTTGCAAAGGTGGCATTGGGGCCGAGGGACCGAACAGAAAACGATTTTCTTTATTATTTCAAATTTGACAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCa                                                                                 <  3:210737/136‑1 (MQ=255)
                                                    accggggcCCCCCATT‑CCTCTTGCAACCCGGGCAGGGCTGCCGCCGGCCCGAACACCAAACTATTTTTTTTATTCTGACCAATTTCACAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCa                                                                                 <  7:368870/135‑1 (MQ=255)
                                                    aagggggcCCCCCTTT‑CCTATTGAAACGCGGGCTGGGCGGCCGCCGGCCCCACCACCAAACTATTTTCTTTATTCTGTCAAATTTTAAAAAAAACCGCTTCCGATATGGAAATCGACGGATATCGAATATGGTCAGGCa                                                                                 <  2:227570/135‑1 (MQ=255)
                                                     accggggGCCCCATTGCCTCTTCCACCGGGGGCAGGGCGGCCCACGGCCAGACCACCAACCCATTTGTTTTATTCTGTCAAATTTGAGAAAAACACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCa                                                                                 <  4:197767/136‑1 (MQ=255)
                                                      cTGGGCCCCCCATT‑CCTCTTGAAACGGGGGCCGTGCTGCCGACGGCCCGAACAGAAAACTATTTTCTTTATTCTGTCAAATTTGAAAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACt                                                                               <  7:333939/139‑1 (MQ=255)
                                                        ggggCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGt                                                                             >  5:332128/1‑139 (MQ=255)
                                                        ggggCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATATTCTTTATTCTGTCCAATTTGAGAAAAAAACCCTTCCGATATGGAAATCAACGGATATCGAATAAGGTCAGGCACTg                                                                              >  7:251621/1‑138 (MQ=255)
                                                        ggggCCCCCATT‑CCGCTTGCAAATCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGAATTTCTATATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGAAATAGAATATGGTCAGGCACTg                                                                              >  4:67013/1‑138 (MQ=255)
                                                        ggggCCCCCATT‑CCGCTTGAAATGGTGGCTGTGAGGCGTCCGCCCCCAACAACAAACGATTTTATTTATTGTGTCAAATTTGATAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGt                                                                             <  7:269977/139‑1 (MQ=255)
                                                          ggCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTAt                                                                           >  3:178348/1‑139 (MQ=255)
                                                             ccccATT‑ACTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTGCCGATATGGAAATCAACGTATATCGAAAATGGTCAGGCACGGTATGaa                                                                        >  8:86845/1‑139 (MQ=255)
                                                                        ctTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGtgtc                                                              >  6:331240/1‑139 (MQ=255)
                                                                         ttGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGtgtct                                                             >  3:207638/1‑139 (MQ=255)
                                                                          tGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGtgtctg                                                            >  1:269641/1‑139 (MQ=255)
                                                                                 cTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGt                                                      >  4:57208/1‑138 (MQ=255)
                                                                                   ggCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTaa                                                   >  3:233785/1‑139 (MQ=255)
                                                                                    gCAGGGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAat                                                  <  8:88262/139‑1 (MQ=255)
                                                                                          ctgcCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCt                                            >  2:63975/1‑139 (MQ=255)
                                                                                             cctagggCCCGAACATAAATGTATTTTATTTATTCTGCGCATTTTCAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGat                                         <  2:260047/134‑1 (MQ=255)
                                                                                               ttgggCCCGTACATCAAACTATTTAATTATTGTTTTCAAATTTCATAAAATACCGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGGCCGGTTAATATGCCTGatat                                       <  8:251621/136‑1 (MQ=255)
                                                                                               gagggCCCTAACAGCAAACGTTTTTATTTTTTGTGTCAAATTTTATAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGatat                                       <  2:67542/136‑1 (MQ=255)
                                                                                               gACGGCCCTAAACCCAAACTATTTTATTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGatat                                       <  4:207638/139‑1 (MQ=255)
                                                                                                 cGGCCCTAACAGAAAAATATTTTTTTTAATTTGACATTTTTCATAAAAACCCGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGatat                                       <  3:67013/137‑1 (MQ=255)
                                                                                                    ccccAACAGCAAAGTATTTTCTTTATTCTGACAATTTTCATAAAACAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCtt                                  <  7:209060/139‑1 (MQ=255)
                                                                                                    cccTAACAGCAAACGATTTTTTTTATTCTGTAAATTTTTATAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCtt                                  <  4:178348/139‑1 (MQ=255)
                                                                                                             cAAACGATTTTCTTTATTCTGTCCTATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTAGGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGt                         >  7:326852/1‑139 (MQ=255)
                                                                                                             cAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGt                         >  6:167748/1‑139 (MQ=255)
                                                                                                                            tttttGTCAAATTTCACAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATa           <  2:110952/134‑1 (MQ=255)
                                                                                                                              tCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATaaa         <  5:167748/138‑1 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGc                                                                                  >  7:67893/1‑61 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGc                                                                                  <  8:67893/61‑1 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACt                                                                               <  6:389875/64‑1 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACt                                                                               >  5:389875/1‑64 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGatat                                       <  1:265619/104‑1 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGatat                                       >  2:265619/1‑104 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATa           <  4:382633/132‑1 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATa           >  3:382633/1‑132 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAAttc     >  8:179076/1‑136 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCa    >  8:372351/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCa    >  7:346324/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCa    >  8:124168/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCa    >  5:275401/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCa    >  5:69031/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCa    >  7:323709/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCa    >  7:118853/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACACTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGc     >  2:48216/1‑138 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGAATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGc     >  5:349408/1‑138 (MQ=255)
                                                                                                                                  tCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATAGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATCCa    >  4:380200/1‑139 (MQ=255)
                                                                                                                                  tCCAATTTGACAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAAt       <  1:72632/136‑1 (MQ=255)
                                                                                                                                    cAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCATa  <  5:331240/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                          
TTCTCCTTGCATTGTCCAAAATCGAAAACGGCACATTCGGATACTGTGAAGAAACTGGGGCCCCCATT‑CCTCTTGCAAAGCTGGCAGTGCTGCCGACGGCCCGAACAGCAAACGATTTTCTTTATTCTGTCCAATTTGAGAAAAAAACGCTTCCGATATGGAAATCAACGGATATCGAATATGGTCAGGCACTGTATGAATAACAGTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCATA  >  BS168_reference_genome/1616439‑1616708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑