Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	2,015,087	C→T	6.5%	S103S (AGC→AGT)	gltC →	transcriptional regulator (LysR family) (GltC‑glutamate)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,015,087	0	C	T	6.5%	200.2 / 2.1	61	S103S (AGC→AGT)	gltC	transcriptional regulator (LysR family) (GltC‑glutamate)
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base T (2/2);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCCAATCGGAAAAGAATTTTTAATTCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGCCTTATTAGGGC  >  BS168_reference_genome/2014949‑2015220                                                                                                                                           |                                                                                                                                      cACGCCAATCGGAAAAGAATTTTTAATTCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCgccagc                                                                                                                                       <  3:304925/139‑1 (MQ=255) cACGCCAATCGGAAAAGAATTTTTAATTCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCgccagc                                                                                                                                       <  5:29272/139‑1 (MQ=255)    gCCAATCGGAAAAGAATTTTTAATTCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGccagccag                                                                                                                                    >  2:415283/1‑139 (MQ=255)         tCGGAAAAGAATTTTTAATTCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCtttt                                                                                                                               >  1:301521/1‑139 (MQ=255)               aaGAATTTTTACTTCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGAc                                                                                                                         <  4:88074/139‑1 (MQ=255)                 gAATTTTTAATTCATGTGAACACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTg                                                                                                                       <  2:370213/139‑1 (MQ=255)                          aTTCATGTTAACTCGGCTTTGTAAGCGATTGATTATGCGAAAGAGCATATAGTGGAGTATGTTGACCCGCTTCGCGGAACGGTAAAGATCGGCTATCCTACAAGCCTCGCCAGCCTGCTTTTGCCGTCTGTGATTTCa                                                                                                               <  8:185950/138‑1 (MQ=255)                            tCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCg                                                                                                            >  1:288706/1‑139 (MQ=255)                              aTGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGtt                                                                                                          >  5:22854/1‑139 (MQ=255)                                                   ccATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGt                                                                                     <  7:237850/139‑1 (MQ=255)                                                       tGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGaa                                                                                 >  7:243597/1‑139 (MQ=255)                                                             tGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGTCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTtg                                                                           >  7:25086/1‑139 (MQ=255)                                                             tGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGTCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTtg                                                                           >  1:27523/1‑139 (MQ=255)                                                              gCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGCCTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTtg                                                                           >  1:238079/1‑138 (MQ=255)                                                               cGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTtgct                                                                         >  4:326272/1‑139 (MQ=255)                                                                     agCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCa                                                                   <  3:278284/139‑1 (MQ=255)                                                                       cAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAg                                                                 <  7:22045/139‑1 (MQ=255)                                                                       cAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAg                                                                 <  1:415283/139‑1 (MQ=255)                                                                       cAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAg                                                                 >  1:213505/1‑139 (MQ=255)                                                                       cAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAg                                                                 >  4:385852/1‑139 (MQ=255)                                                                       cAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTAAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGACaat                                                                 >  4:298164/1‑138 (MQ=255)                                                                        aaaTTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAgg                                                                <  7:59036/139‑1 (MQ=255)                                                                        aaaTTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAgg                                                                <  4:422120/139‑1 (MQ=255)                                                                        aaaTTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAgg                                                                <  1:205183/139‑1 (MQ=255)                                                                               tgaGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCta                                                          >  4:406011/1‑138 (MQ=255)                                                                                   tATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGt                                                     >  1:80325/1‑139 (MQ=255)                                                                                          aCCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAaag                                                                                                     <  7:303936/84‑1 (MQ=255)                                                                                          aCCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAaag                                                                                                     >  8:303936/1‑84 (MQ=255)                                                                                             cGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTg                                            >  6:156040/1‑138 (MQ=255)                                                                                             cGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGa                                           <  5:156040/139‑1 (MQ=255)                                                                                               cATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAg                                         <  3:326272/139‑1 (MQ=255)                                                                                               cATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAg                                         >  3:21786/1‑139 (MQ=255)                                                                                               cATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAg                                         <  2:301521/139‑1 (MQ=255)                                                                                               cATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAAGTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGAtggcag                                         >  4:366799/1‑134 (MQ=255)                                                                                               cATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCGCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAg                                         <  6:80532/139‑1 (MQ=255)                                                                                                            tAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACcg                            <  8:128898/139‑1 (MQ=255)                                                                                                            tAAAGATCGGCTCTCCTACAAGCCTCGACAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACcg                            <  6:170313/139‑1 (MQ=255)                                                                                                              aaGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACcgc                           >  7:379283/1‑138 (MQ=255)                                                                                                                 aTCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACcg                            <  2:156994/134‑1 (MQ=255)                                                                                                                 aTCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACcg                            >  1:156994/1‑134 (MQ=255)                                                                                                                 aTCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGata                       <  8:243597/139‑1 (MQ=255)                                                                                                                 aTCGGCTTACCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAACTTTCTGATTGAAGCTGTCAGAAACCGCtata                       >  8:313203/1‑139 (MQ=255)                                                                                                                   cGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATAtt                     >  4:132024/1‑139 (MQ=255)                                                                                                                     gCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGa                   >  6:416062/1‑139 (MQ=255)                                                                                                                      ctctCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGAt                  <  3:6512/136‑1 (MQ=255)                                                                                                                      cTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAg                                                                 <  2:305573/92‑1 (MQ=255)                                                                                                                      cTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAg                                                                 >  1:305573/1‑92 (MQ=255)                                                                                                                      cTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGata                       >  3:110679/1‑134 (MQ=255)                                                                                                                      cTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGata                       <  4:110679/134‑1 (MQ=255)                                                                                                                      cTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGAt                  <  6:216637/139‑1 (MQ=255)                                                                                                                      cTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGAt                  <  6:420561/139‑1 (MQ=255)                                                                                                                      cTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGAt                  <  2:80325/139‑1 (MQ=255)                                                                                                                         tCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATc                 <  3:252005/137‑1 (MQ=255)                                                                                                                           cTACAAGCCTCGCCAGTCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGc             <  2:27523/139‑1 (MQ=255)                                                                                                                           cTACAAGCCTCGCCAGTCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGc             <  8:25086/139‑1 (MQ=255)                                                                                                                           cTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCATAAACCGCGATATTGATCTGGc             <  4:21786/139‑1 (MQ=255)                                                                                                                           cTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGc             <  2:213505/139‑1 (MQ=255)                                                                                                                           cTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGc             <  3:385852/139‑1 (MQ=255)                                                                                                                                aGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACAGCGATATTGATCTGGCCttat        >  1:298685/1‑139 (MQ=255)                                                                                                                                 gCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGCCttatt       <  6:22854/139‑1 (MQ=255)                                                                                                                                 gCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGCCttatt       <  3:298164/139‑1 (MQ=255)                                                                                                                                 gCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGCCttatt       <  5:416062/139‑1 (MQ=255)                                                                                                                                    tCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGCCTTATTAgt    >  8:387266/1‑138 (MQ=255)                                                                                                                                     cGCCAGCCAGCTTTTGCAGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAATTTTCTGATAGAAGCTGTCAGAAACCGCGATATTGATCTGGCCTTATTAggg   >  8:116752/1‑139 (MQ=255)                                                                                                                                      gccagccaGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGCCTTATTAGGGc  >  8:379427/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                      CACGCCAATCGGAAAAGAATTTTTAATTCATGTGAAAACGGCGATGAAAGCCATTGATTATGCGAAAGAGCAAATTGATGAGTATCTTGACCCGCATCGCGGAACGGTAAAGATCGGCTTTCCTACAAGCCTCGCCAGCCAGCTTTTGCCGACTGTCATTTCAGCGTTTAAAGAAGAATATCCGCACGTCGAATTTTTGCTGCGCCAAGGCTCCTATAAGTTTCTGATTGAAGCTGTCAGAAACCGCGATATTGATCTGGCCTTATTAGGGC  >  BS168_reference_genome/2014949‑2015220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑