Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	2,364,928	A→T	7.2%	V55V (GTT→GTA)	qcrA ←	menaquinol:cytochrome c oxidoreductase (iron‑sulfur subunit)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,364,928	0	A	T	7.2%	186.7 / 4.6	65	V55V (GTT→GTA)	qcrA	menaquinol:cytochrome c oxidoreductase (iron‑sulfur subunit)
Reads supporting (aligned to +/- strand):  ref base A (26/34);  new base T (2/3);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

TACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCATGAGCATACTAGCCGCCATAAAACCTCCTACGCCTGTGAGCGTATAATTCAAAAATTGACGT  >  BS168_reference_genome/2364792‑2365066                                                                                                                                         |                                                                                                                                           tACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGCCCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAAc                                                                                                                                          >  8:255708/1‑139 (MQ=255) tACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTTACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAAc                                                                                                                                          >  7:139065/1‑139 (MQ=255) tACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAAc                                                                                                                                          >  6:278050/1‑139 (MQ=255) tACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAAc                                                                                                                                          >  5:216059/1‑139 (MQ=255) tACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAAc                                                                                                                                          >  7:371463/1‑139 (MQ=255) tACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCGCTTTTGTCAACTCATCAAc                                                                                                                                          >  4:9758/1‑139 (MQ=255) tACAAATTGGCGAAAGTGCTACAATATCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATAAATATTAAAATCAAAGAGCTGAGGCTCTTTTGTCAACTCATCAAc                                                                                                                                          >  4:107244/1‑139 (MQ=255)  aCAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCaa                                                                                                                                           <  2:358824/137‑1 (MQ=255)     aaTTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTg                                                                                                                                      <  5:379840/139‑1 (MQ=255)      aTTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGa                                                                                                                                     <  3:270118/139‑1 (MQ=255)          gCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAAc                                                                                                                                 <  8:301245/139‑1 (MQ=255)            gAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCt                                                                                                                               <  5:5542/139‑1 (MQ=255)            gAAAGTGCTACAATTTCATCCCCGTTGTTGAAGACCCAGGCTGATCTTGCCTCATCTGACTCATACCAGGCATCGACATGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACACCCt                                                                                                                               <  4:37689/139‑1 (MQ=255)               aGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGaa                                                                                                                            <  8:380731/139‑1 (MQ=255)                                   gTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTc                                                                                                        <  4:104902/139‑1 (MQ=255)                                   gTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTc                                                                                                        <  6:216059/139‑1 (MQ=255)                                   gTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTc                                                                                                        >  4:213495/1‑139 (MQ=255)                                   gTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTc                                                                                                        <  2:178027/139‑1 (MQ=255)                                   gTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTc                                                                                                        <  3:310128/139‑1 (MQ=255)                                   gTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCAGAGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTc                                                                                                        <  7:186490/139‑1 (MQ=255)                                                   gCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGt                                                                                        <  8:301123/139‑1 (MQ=255)                                                    cTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTACCCTGTCGATTTTAATACCGGGTc                                                                                       <  3:9758/139‑1 (MQ=255)                                                       aTCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGACCCATCTCCTGCTTCCCTGTCGATTTTTATAACGGGTCGAg                                                                                    >  7:108957/1‑139 (MQ=255)                                                                 ttctGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           >  3:58425/1‑138 (MQ=255)                                                                   ctGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           <  7:83763/136‑1 (MQ=255)                                                                   ctGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           <  7:133909/136‑1 (MQ=255)                                                                   ctGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           <  7:243668/136‑1 (MQ=255)                                                                   ctGACTCATACCAGGCATCGACTTGATTAAATTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGGCGAGTgcgaagcg                                                                           <  5:310102/136‑1 (MQ=255)                                                                   ctGAATCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           <  4:10919/136‑1 (MQ=255)                                                                      aCTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGTGTCGAGTGCGAAGCGAACCa                                                                      <  5:265711/138‑1 (MQ=255)                                                                      aCTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCa                                                                      <  1:218638/138‑1 (MQ=255)                                                                          aTACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGc                                                                 <  5:278050/139‑1 (MQ=255)                                                                           tACCAGGCATCGCCTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGCCAACCTGAACCATATCCTGCTTCCCTGTCGAGTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCa                                                                >  6:410917/1‑139 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACTCTGACAACCTGAACCAGATCCTGCTTCCCTGTCGATTTTAAAAACGGGTCGGGGGCGAAGCGAACCaatggg                                                                 >  7:166794/1‑133 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGACCCATATCCTGCTTCCCTGTCGATTTTAAAACCGGGTCGAGTGCGAAGCGAACCATAGGCa                                                                >  4:394968/1‑139 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCata                                                                                                                       >  8:274152/1‑84 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCata                                                                                                                       <  7:274152/84‑1 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATAcc                                                                                            <  5:209816/111‑1 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATAcc                                                                                            >  6:209816/1‑111 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           <  6:209321/128‑1 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           >  6:198334/1‑128 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           >  5:209321/1‑128 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           <  5:198334/128‑1 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCa                                                                >  6:217050/1‑139 (MQ=255)                                                                           tACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATAACGGGTCGATTTCGCAGCGAACCCTAGGc                                                                 >  8:353366/1‑138 (MQ=255)                                                                             ccAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCAt                                                               <  7:255708/138‑1 (MQ=255)                                                                                ggCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           >  2:79008/1‑123 (MQ=255)                                                                                ggCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaagcg                                                                           <  1:79008/123‑1 (MQ=255)                                                                                ggCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTgcgaag                                                                             <  8:363383/121‑1 (MQ=255)                                                                                ggCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACAGGGTCGAGTgcgaag                                                                             >  7:363383/1‑121 (MQ=255)                                                                                  cATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCACCGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGAGTTTAAGACCGGGGCGAGTGGGAAGCGCACCATACGCATGAGCa                                                          >  4:362648/1‑138 (MQ=255)                                                                                  cATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCa                                                                      <  8:176764/126‑1 (MQ=255)                                                                                  cATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCa                                                                      >  7:176764/1‑126 (MQ=255)                                                                                     cGACTTGGTTAATTTTGAAATCAAAGCGCTGCGGCTCTTTTGTCAGCTCATCTACGCTGACAACCTGAACCATGTCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCAAAGCGAAcc                                                                       >  7:252226/1‑122 (MQ=255)                                                                                     cGACTTGGTTAATTTTGAAATCAAAGCGCTGCGGCTCTTTTGTCAGCTCATCTACGCTGACAACCTGAACCATGTCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTCCAAAGCGAAcc                                                                       <  8:252226/122‑1 (MQ=255)                                                                                        cTTGGTTAATTTTGAAATCAAAGCTCTGCTGCTCTTTTGTCAGCTCATCTACGATGACAACCTGAACCATGTCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTCCAAAGCGAACCATAGGCATGAGCATACTAgc                                                   <  8:323484/139‑1 (MQ=255)                                                                                        cTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCATGAGCATACTAgc                                                   <  1:399939/139‑1 (MQ=255)                                                                                                   ttAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCATGAGCATACTAGCCGCCATaaaa                                         <  4:41218/138‑1 (MQ=255)                                                                                                   ttAAAATCAAAGCGCGGCGGCTCTTTTGGCAACTCATCAACGCTGACAACCTGAACCATATCCTGCGTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCATGAGCATACTAGCCGCCATaaaa                                         <  6:147681/138‑1 (MQ=255)                                                                                                      aaaTCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGAttt                                                                                                   <  3:210031/77‑1 (MQ=255)                                                                                                      aaaTCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGAttt                                                                                                   >  4:210031/1‑77 (MQ=255)                                                                                                         tCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCATGAGCATACTAGCCGCCATAAAACCTCCTa                                  <  8:139065/139‑1 (MQ=255)                                                                                                           aaaGCGCTGCGGCTCTTTTGTCAGCTCATCTACGCTGACAACCTGAACCATGTCCTGCTTCCCTGTc                                                                                                        <  8:210565/67‑1 (MQ=255)                                                                                                           aaaGCGCTGCGGCTCTTTTGTCAGCTCATCTACGCTGACAACCTGAACCATGTCCTGCTTCCCTGTc                                                                                                        >  7:210565/1‑67 (MQ=255)                                                                                                                  tGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCATGAGCATACTAGCCGCCATAAAACCTCCTACGCCtgt                           >  7:226561/1‑137 (MQ=255)                                                                                                                                 aaCTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCAAGAGCATACTAGCCGCCATAAAACCTCCTACGCCTGTGAGCGTATAATTCaaa           >  8:181032/1‑138 (MQ=255)                                                                                                                                      atcaACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTATTACCGGGTCGAGTGCGAAGCGAACAATAGGCATGAGCATACTAGCCGCCATAAAACCTCCTACGCCTGTGAGCGTATAATTCAAAAAtt       <  3:107244/137‑1 (MQ=255)                                                                                                                                      atcaACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCa                                                                      <  3:342542/74‑1 (MQ=255)                                                                                                                                      atcaACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCa                                                                      >  4:342542/1‑74 (MQ=255)                                                                                                                                         aaCGCTGACAACCTTAACCATATCCTGCTTCCCTTTAGATTTTAATACCGGGTCGAGTGCGAAGCGAACAATAGGCATGAGCTTACTAGCCGCCATAAAACCTCATACGCCTGTGAGCGTATAATTCAAAAATTGACGt  <  8:166794/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                           TACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAATCAAAGCGCTGCGGCTCTTTTGTCAACTCATCAACGCTGACAACCTGAACCATATCCTGCTTCCCTGTCGATTTTAATACCGGGTCGAGTGCGAAGCGAACCATAGGCATGAGCATACTAGCCGCCATAAAACCTCCTACGCCTGTGAGCGTATAATTCAAAAATTGACGT  >  BS168_reference_genome/2364792‑2365066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑