Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	2,732,512	T→C	7.3%	pseudogene (36/159 nt)	yrdDc ←	hypothetical protein; C‑terminal part of YrdD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,732,512	0	T	C	7.3%	169.3 / 4.2	55	pseudogene (36/159 nt)	yrdDc	hypothetical protein; C‑terminal part of YrdD
Reads supporting (aligned to +/- strand):  ref base T (22/29);  new base C (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

TCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGATCAAGTTTAATGTCGGGTTTATCAC  >  BS168_reference_genome/2732374‑2732650                                                                                                                                           |                                                                                                                                           tCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTACACTCttt                                                                                                                                            >  2:195759/1‑139 (MQ=255) tCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCttt                                                                                                                                            >  5:198438/1‑139 (MQ=255) tCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCttt                                                                                                                                            >  6:219625/1‑139 (MQ=255) tCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCttt                                                                                                                                            >  3:1785/1‑139 (MQ=255) tCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCttt                                                                                                                                            >  1:83981/1‑139 (MQ=255) tCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGATGCCTACACTCttt                                                                                                                                            >  6:28420/1‑139 (MQ=255)     tAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCt                                                                                                                                        >  2:253956/1‑139 (MQ=255)             aaaTCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATaaaa                                                                                                                                 <  6:387927/138‑1 (MQ=255)                  aGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCg                                                                                                                            <  7:232844/138‑1 (MQ=255)                  aGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGa                                                                                                                           <  3:234025/139‑1 (MQ=255)                   ggACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGaa                                                                                                                          <  5:280962/139‑1 (MQ=255)                   ggACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGaa                                                                                                                          <  5:112631/139‑1 (MQ=255)                    gACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGaaa                                                                                                                         <  4:248599/139‑1 (MQ=255)                    gACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGaaa                                                                                                                         >  6:31959/1‑139 (MQ=255)                    gACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGaaa                                                                                                                         <  8:329561/139‑1 (MQ=255)                    gACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGaaa                                                                                                                         >  5:213844/1‑139 (MQ=255)                         tttGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTcc                                                                                                                     >  8:333069/1‑138 (MQ=255)                            gTAAAATGATCCATTGAGATCAGTATTCATGATCAGCGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTaa                                                                                                                  <  6:237027/138‑1 (MQ=255)                            gTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTaa                                                                                                                  <  4:125488/138‑1 (MQ=255)                            gTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTaa                                                                                                                  <  6:198438/138‑1 (MQ=255)                                         ttGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCgg                                                                                                    <  2:83981/139‑1 (MQ=255)                                         ttGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCgg                                                                                                    <  7:376022/139‑1 (MQ=255)                                              aTCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCtt                                                                                               >  3:211642/1‑139 (MQ=255)                                              aTCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCtt                                                                                               >  1:167365/1‑139 (MQ=255)                                                   tagtCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGACACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAACTGGCGAAACTCCTAATACTTCTATGACGGCTCTTTTAAg                                                                                          >  8:64714/4‑139 (MQ=255)                                                   tATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAg                                                                                          >  7:273303/1‑139 (MQ=255)                                                        aTGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGAttt                                                                                     >  6:336986/1‑139 (MQ=255)                                                        aTGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGAGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGAttt                                                                                     >  6:140792/1‑139 (MQ=255)                                                             aaGGGCCAAAATGCCGTCCCCGCATTTTTTAATTAAAGATATGCGACAGTCATTAATTATTTTGCTGCCTAAAATCTTTGCCTTATAAAATGGCGAAACTCCTAATACTTCAATGCCCGCTCTTTTATGGATTTTGaa                                                                                 >  7:156982/1‑138 (MQ=255)                                                             aaGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGaa                                                                                 >  1:192998/1‑138 (MQ=255)                                                             aaGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGaa                                                                                 >  5:360728/1‑138 (MQ=255)                                                             aaGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGaa                                                                                 >  4:189231/1‑138 (MQ=255)                                                             aaGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTCCCCTGATAAAATGGCGaaa                                                                                                                         >  8:102271/1‑98 (MQ=255)                                                             aaGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTCCCCTGATAAAATGGCGaaa                                                                                                                         <  7:102271/98‑1 (MQ=255)                                                             aaGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGACATTAATCATTTTGCTGCCTAAACTCTTCCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGaa                                                                                 >  2:413816/1‑138 (MQ=255)                                                                          ccGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGccc                                                                    >  8:172786/1‑138 (MQ=255)                                                                            gTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCAt                                                                 >  8:181829/1‑139 (MQ=255)                                                                             tCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCagt                                                                >  1:406984/1‑137 (MQ=255)                                                                             tCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCAtt                                                                <  5:219625/139‑1 (MQ=255)                                                                             tCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCAtt                                                                <  3:189231/139‑1 (MQ=255)                                                                                    aTTTTGAAATAAAATATATGAGACAGTCATTAATCATTTTTCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTa                                                         <  5:188227/139‑1 (MQ=255)                                                                                    aTTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTa                                                         <  1:253956/139‑1 (MQ=255)                                                                                    aTTTTGAAATAAAAGATATGAGACAGACATTAATCATTTTGCTGCCTAAACTCTTCCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTa                                                         <  1:413816/139‑1 (MQ=255)                                                                                          aaaTAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAACTGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTa                                                   <  8:284939/139‑1 (MQ=255)                                                                                           aaTAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTAt                                                  <  6:254468/139‑1 (MQ=255)                                                                                           aaTAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTAt                                                  <  5:336986/139‑1 (MQ=255)                                                                                              aaaaGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATccc                                               <  4:1785/139‑1 (MQ=255)                                                                                                aaGATATGAGTCAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATccccc                                             <  8:156982/139‑1 (MQ=255)                                                                                                aaGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTACTACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTCTccccc                                             >  6:26439/1‑139 (MQ=255)                                                                                                aaGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATccccc                                             >  6:20667/1‑139 (MQ=255)                                                                                                 aGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATcccccc                                            <  6:213844/139‑1 (MQ=255)                                                                                                 aGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATcccccc                                            <  7:172786/139‑1 (MQ=255)                                                                                                    tatGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCctgc                                         <  5:31959/139‑1 (MQ=255)                                                                                                                   aaTCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTAt                                                  >  1:195301/1‑115 (MQ=255)                                                                                                                   aaTCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTAt                                                  <  2:195301/115‑1 (MQ=255)                                                                                                                    aTCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGAt                          <  2:192998/138‑1 (MQ=255)                                                                                                                    aTCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGAt                          <  1:195759/138‑1 (MQ=255)                                                                                                                    aTCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGAt                          <  5:119538/138‑1 (MQ=255)                                                                                                                    aTCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGATc                         <  8:273303/139‑1 (MQ=255)                                                                                                                          ttGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGACGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGATCAAGtt                    >  1:62530/1‑138 (MQ=255)                                                                                                                               gcCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGATCAAGTTTAATGt              >  8:22242/1‑139 (MQ=255)                                                                                                                                          ttCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCctgctg                                       >  3:110458/1‑103 (MQ=255)                                                                                                                                          ttCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCctgctg                                       <  4:110458/103‑1 (MQ=255)                                                                                                                                          ttCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGATCAAGTTTAATGTCGGGTTTATCa   >  2:138728/1‑139 (MQ=255)                                                                                                                                          ttCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATGATTGTTATCCCCCCTGCTGCTATATAATCGATCAAGTTTATTGTCGGGTTTTTc    >  4:104951/1‑138 (MQ=255)                                                                                                                                           tCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGATCAAGTTTAATGTCGGGTTTATCAc  >  3:298481/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                           TCTTTAAATATAAAATCAGGACGATTTGTAAAATGATCCATTGAGATCAGTATTCATGATAAGGGCCAAAATGCCGTCCCCGCATTTTGAAATAAAAGATATGAGACAGTCATTAATCATTTTGCTGCCTAAACTCTTTCCCTGATAAAATGGCGAAACTCCTAATACTTCTATGCCGGCTCTTTTAAGGATTTGGAAAATTTTTTAGCCCCATTTTGATTAATGTTATCCCCCCTGCTGCTATATAATCGATCAAGTTTAATGTCGGGTTTATCAC  >  BS168_reference_genome/2732374‑2732650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑