| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 3,114,257 | A→G | 6.4% | L345L (TTG→CTG) | ytrF ← | metabolite permease involved in resistance to cell wall inhibitors |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 3,114,257 | 0 | A | G | 6.4% | 195.0 / 3.8 | 62 | L345L (TTG→CTG) | ytrF | metabolite permease involved in resistance to cell wall inhibitors |
| Reads supporting (aligned to +/- strand): ref base A (35/23); new base G (2/2); total (37/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
ATCGCCCGCATCGCCACCGCTTGTGGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGATAACTGCGATACATCCGA > BS168_reference_genome/3114126‑3114391 | aTCGCCCGCATCGCCACCGCTTGTGGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACAtt > 4:374850/1‑139 (MQ=255)aTCGCCCGCATCGCCACCGCTTGTGGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACAtt > 4:398446/1‑139 (MQ=255)aTCGCCCGCATCGCCACCGCTTGTGGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTGTCCATCAAGAACAtt > 2:72306/1‑139 (MQ=255) ccGCATCGCCACCGCTTGTGGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCg < 5:2883/139‑1 (MQ=255) aCCGCTGGTGGCCGCAAGAATCCTCGGAACGGCCAGGGTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGAc < 4:1666/139‑1 (MQ=255) cGCTTGTGCCCGCAAGATTCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACgg > 7:171987/1‑139 (MQ=255) tgtgGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTc > 4:375796/1‑139 (MQ=255) gtgGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCt < 3:392919/137‑1 (MQ=255) gtgGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCg > 2:177019/1‑139 (MQ=255) cAAGAATCATCGGCACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAAt < 5:57886/139‑1 (MQ=255) cAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAAt > 6:320658/1‑139 (MQ=255) gAATCATCGGTACGGCCATGTTGACTAGGTAGCTCTCGCCGTAAGAAATGATAATCCCAATCACACAGCCTAATTTTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGTCGGGCTCGCCCCAATCGc < 4:108445/139‑1 (MQ=255) aaCGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATg > 7:402774/1‑139 (MQ=255) aaCGGCCAGGATGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATg < 6:99327/139‑1 (MQ=255) ggCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCCCACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCg < 2:20460/139‑1 (MQ=255) ggCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCg < 4:55160/139‑1 (MQ=255) ccAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGAt < 2:258483/139‑1 (MQ=255) aCAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATACCGATATACTCGCTTTCCAGCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAAGCGCTTTCATAATCCCGATCTCTAGCg > 1:409791/1‑139 (MQ=255) cAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGt < 8:171987/139‑1 (MQ=255) aaGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGTTATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGtt < 8:227746/139‑1 (MQ=255) ggTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTtct > 7:32518/1‑139 (MQ=255) cTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCt > 3:279790/1‑95 (MQ=255) cTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCt < 4:279790/95‑1 (MQ=255) aCGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCa < 8:85594/139‑1 (MQ=255) aCGCCGTAAGAAAGGATAATCCCAATCACACAGCCAATACTTCCGATATACGCGCTTTACATCAAGAACATTCGGCGAATGATTGACTGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGt < 7:348232/137‑1 (MQ=255) gCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGcc > 1:333699/1‑114 (MQ=255) gCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGcc < 2:333699/114‑1 (MQ=255) gCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCAcc > 7:313106/1‑139 (MQ=255) tAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCca > 6:308524/1‑139 (MQ=255) aTGATAATCCCAATCCCACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACACTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCGCTCTGTCACCGCcatcgt > 1:348896/1‑137 (MQ=255) tGATAATCCCAATCACAGATCCTAAAATTCCGATATACGCGCTTTCCATAAAGTACATTGGGCTAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCAGATCTCTTGCGTTCTCTCTGTCACCGCcatcgtcat < 7:84984/139‑1 (MQ=255) gATAATCCCAATCACACAGCCTAAAATTCCGATATACGAGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCcatcgtcatc > 4:40585/1‑139 (MQ=255) tCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCACTCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCgtgt > 4:259282/1‑138 (MQ=255) cccAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGtt > 4:92564/1‑138 (MQ=255) aTCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTaaaaa > 6:231249/1‑139 (MQ=255) tCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGtt < 1:213695/104‑1 (MQ=255) tCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGtt > 2:213695/1‑104 (MQ=255) caGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGa > 8:246420/1‑139 (MQ=255) aGCCTAAAATTCCGATATACGCGCTTTCCATCAGGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGAt > 8:281869/1‑139 (MQ=255) aGCCTAAAATTCCGATATACGCGCTTTCCATCAGGAACATTCGGCGAATGATTGACGGGCTCGCCCCAAACGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCCTGTTAAAAATTACGAt > 8:275512/1‑139 (MQ=255) ccTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCg > 8:191173/1‑139 (MQ=255) ccTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCg > 1:5946/1‑139 (MQ=255) ccTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCg > 7:275266/1‑139 (MQ=255) aaaTTCCGATATACGCGCTTTCCATCAGGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCt < 7:275512/137‑1 (MQ=255) aaaTTCCGATATACGCGCTTTCCATCAGGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCt < 7:281869/137‑1 (MQ=255) aaaTTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCt < 8:26844/137‑1 (MQ=255) aaaCTCCGATCTACGCGCGTTCCATCAAGAACAATCGGCGAATGATTGACGGGCGCGCCCCACTCGCTATCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCTTCGTGTTAAAAATGCCGATCGCt < 6:95570/137‑1 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGccc > 4:348315/1‑54 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGccc < 3:348315/54‑1 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTtct > 7:158858/1‑88 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTtct < 8:158858/88‑1 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGa > 3:263791/1‑138 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGa > 1:240269/1‑138 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGa > 3:278132/1‑138 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGa > 6:262011/1‑138 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGa > 7:163692/1‑138 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGa > 1:98736/1‑138 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGAt < 1:177019/139‑1 (MQ=255) cGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGGGTTAAACATGCCGATCGCTGAAATGa > 3:339164/1‑138 (MQ=255) ttCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGATAACTGCGATACAt > 3:70089/1‑139 (MQ=255) ccATCAAGCAAAAGCGGCGAAGGATTGACGGGCTCGCCCCAATCGCATTCATCATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGGCATCGTGTTAAAAATGCCGATCGCTGAAATGATAACTGCGATACATc < 2:409791/138‑1 (MQ=255) aTCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGATAACTGCGATACATCCGa > 8:152998/1‑139 (MQ=255) | ATCGCCCGCATCGCCACCGCTTGTGGCCGCAAGAATCATCGGAACGGCCAGGTTGACAAGGTAGCTCACGCCGTAAGAAATGATAATCCCAATCACACAGCCTAAAATTCCGATATACGCGCTTTCCATCAAGAACATTCGGCGAATGATTGACGGGCTCGCCCCAATCGCTTTCATAATGCCGATCTCTTGCGTTCTCTCTGTCACCGCCATCGTCATCGTGTTAAAAATGCCGATCGCTGAAATGATAACTGCGATACATCCGA > BS168_reference_genome/3114126‑3114391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |