Predicted mutation
evidence seq id position mutation annotation gene description
RA Exported 2,186 (T)8→7 intergenic (–/‑792) – / → eryR –//vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,1790T.99.0% 24659.0 / 207.2 5922intergenic (–/‑799)–/eryR–//vntifkey=4
Reads supporting (aligned to +/- strand):  ref base T (23/34);  new base . (2530/3313);  total (2561/3361)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5981 total aligned reads displayed.

AAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAG  >  Exported/2042‑2313
                                                                                                                                         |                                                                                                                                      
               gttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGc                                                                                                                           <  7:215958/135‑1 (MQ=255)
               gttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGt                                                                                                                        <  1:291824/138‑1 (MQ=255)
               gttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCAGt                                                                                                                        >  1:47997/1‑138 (MQ=255)
                tttCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTc                                                                                                                       >  2:272960/1‑138 (MQ=255)
                   cTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTc                                                                                                                       >  8:214891/1‑135 (MQ=255)
                      tACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAAttt                                                                                                                 <  4:62169/138‑1 (MQ=255)
                      tACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAAttt                                                                                                                 <  2:156015/138‑1 (MQ=255)
                       aCGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTtct                                                                                                               >  4:164227/1‑139 (MQ=255)
                        cGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAAttt                                                                                                                 >  7:247199/1‑136 (MQ=255)
                         gTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTtc                                                                                                                <  8:74178/136‑1 (MQ=255)
                          tGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTccc                                                                                                            >  6:207347/1‑139 (MQ=255)
                            cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCt                                                                                                           >  6:49558/1‑138 (MQ=255)
                            cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCt                                                                                                           >  6:256622/1‑138 (MQ=255)
                                 taAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctc                                                                                                     <  5:178744/139‑1 (MQ=255)
                                   aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAATAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  2:96889/139‑1 (MQ=255)
                                   aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtct                                                                                                      <  7:273120/136‑1 (MQ=255)
                                   aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  3:79244/139‑1 (MQ=255)
                                   aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  1:100223/139‑1 (MQ=255)
                                   aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  1:211738/139‑1 (MQ=255)
                                      ccGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGtctc                                                                                                >  3:52880/1‑139 (MQ=255)
                                       cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTctct                                                                                               >  5:248491/1‑139 (MQ=255)
                                         ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtct                                                                                                      <  8:326796/130‑1 (MQ=255)
                                         ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  4:383252/133‑1 (MQ=255)
                                         ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                              >  6:282415/1‑138 (MQ=255)
                                         ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                              >  5:121173/1‑138 (MQ=255)
                                         ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                              >  4:217928/1‑138 (MQ=255)
                                          gAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTctct                                                                                               >  7:136209/1‑136 (MQ=255)
                                          gAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTctct                                                                                               >  8:364996/1‑136 (MQ=255)
                                            aaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                              >  7:76761/1‑135 (MQ=255)
                                                  tttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctc                                                                                                     <  7:191742/122‑1 (MQ=255)
                                                         cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTcc                                                                                                             >  2:137926/1‑107 (MQ=255)
                                                           ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   >  2:349639/1‑115 (MQ=255)
                                                            tAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattttat                                                                              >  8:191603/1‑135 (MQ=255)
                                                             aGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATa                                                                          <  5:150011/138‑1 (MQ=255)
                                                             aGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATa                                                                          <  5:47855/138‑1 (MQ=255)
                                                                aGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATaa                                                                         <  7:166750/136‑1 (MQ=255)
                                                                aGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  4:94324/139‑1 (MQ=255)
                                                                    caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCttt                                                                                             >  5:336902/1‑112 (MQ=255)
                                                                    caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAATGGAGTa                                                                  >  1:340635/1‑139 (MQ=255)
                                                                            gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATaa                                                                         >  1:240779/1‑124 (MQ=255)
                                                                            gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAcc                                                          >  3:249367/1‑139 (MQ=255)
                                                                                 atGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   >  4:109771/1‑93 (MQ=255)
                                                                                  tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                    <  3:182700/139‑1 (MQ=255)
                                                                                  tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                    <  3:373564/139‑1 (MQ=255)
                                                                                     aTCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATAATAAAGGAGTATTTGAACCGTcgcg                                                    >  7:299278/1‑136 (MQ=255)
                                                                                      tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATGTCGTCGCGTTTTATGTTTTTTTTTTCATTAATGGGTTTTTTAAACGTGGGGCggg                                                >  2:190002/1‑139 (MQ=255)
                                                                                      tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   >  3:297462/1‑88 (MQ=255)
                                                                                      tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                 >  2:216998/1‑138 (MQ=255)
                                                                                      tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAACGTCGCGCgg                                                 >  5:36864/1‑138 (MQ=255)
                                                                                       agataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  1:172069/137‑1 (MQ=255)
                                                                                         atcaTGATTTGTTGAAGTTTTGCCACAACAGTTGGGTTATTTACAATC‑TTTTTTTATTAGCAGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  1:150613/135‑1 (MQ=255)
                                                                                         ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCggg                                                >  7:50053/1‑136 (MQ=255)
                                                                                         ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                 >  7:360047/1‑135 (MQ=255)
                                                                                         ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                 >  8:100879/1‑135 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   >  4:270921/1‑84 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcgcg                                                  <  8:162630/133‑1 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  2:322213/136‑1 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  5:305201/137‑1 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAg                                            >  1:365581/1‑139 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAg                                            >  6:368374/1‑139 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTAATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  5:228319/136‑1 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCATTATTTTTTAATAAATTAGTATTTTAACAGTCGAGCGGGaa                                              >  6:231660/1‑136 (MQ=255)
                                                                                          taATGATTTGTGGAAGTTTTGCCACAACAGTTGGGTTATTTACAATC‑TTTTTTTATTAGCCGTCTAATTTCTCCCTCATCTCGTCTCTTAATATCTTTTTTGTATAATAAATGAGTATTTGAACCGTCGCGCGGGACAg                                            >  1:75824/1‑139 (MQ=255)
                                                                                          taATGATGTGTGGAAGTTTTGCCACAACAGGTGGGTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTTTATCTTTATTTTATGATAAAGGTGTATTTGAACCGTCGCGCGGGa                                               <  6:12819/136‑1 (MQ=255)
                                                                                            aTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGt                                          <  4:357207/139‑1 (MQ=255)
                                                                                            aTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGt                                          <  2:293344/139‑1 (MQ=255)
                                                                                               aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt                                        <  2:110259/138‑1 (MQ=255)
                                                                                               aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTGTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTa                                       >  6:102736/1‑139 (MQ=255)
                                                                                                tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTATTTTTATCTTTTTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGgtttt                                      >  1:128889/1‑137 (MQ=255)
                                                                                                  tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                    >  3:350323/1‑139 (MQ=255)
                                                                                                  tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTATTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                    >  3:322633/1‑139 (MQ=255)
                                                                                                   gtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTa                                       <  8:132489/135‑1 (MQ=255)
                                                                                                    tgGAAGTTTTGCCACAACAGTTTGTTTATTTACAATC‑TTTTTTTAATAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                   <  3:1508/138‑1 (MQ=255)
                                                                                                    tgGAAGTTTTGCCACAACAGTTGTTTTATTTACAATC‑TTTTTTTATTAGCCGGCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTAATTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                   <  5:77174/138‑1 (MQ=255)
                                                                                                    tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttt                                                                                >  6:303773/1‑93 (MQ=255)
                                                                                                    tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                    >  6:338250/1‑137 (MQ=255)
                                                                                                    tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                    >  6:154938/1‑137 (MQ=255)
                                                                                                    tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATATTTATTTTATAATAAATTAGTATTTGAACCGTCGCTCGGGACAGGTTTATGATa                                  >  2:379485/1‑139 (MQ=255)
                                                                                                      gAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                 <  1:27008/138‑1 (MQ=255)
                                                                                                       aaGTTTTGCCACCACAGTTGGTTTCTTTACACTC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCCTCTCGTCTCTATATATCTTTATTATATCATAAAGGACTATTTGACCCGGCGCGCGGGACAGGTTTATGATAggg                               <  2:64569/139‑1 (MQ=255)
                                                                                                       aaGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                 >  8:270507/1‑121 (MQ=255)
                                                                                                          ttttGCCACATGAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAGCGTCGCGCGGGACAGGTTTATGATAGGGata                            <  3:217255/139‑1 (MQ=255)
                                                                                                          ttttGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGata                            <  3:128986/139‑1 (MQ=255)
                                                                                                              gCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATa                                                                          >  4:18857/1‑89 (MQ=255)
                                                                                                               ccACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                       <  6:7908/139‑1 (MQ=255)
                                                                                                                         tGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTTTTTTTTCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGGTAGGGATATTTTATTGATTAttt             >  3:271352/1‑139 (MQ=255)
                                                                                                                           gTTTATTTACTATC‑TTTTTTTATTAGCCGTAAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataat              <  8:478/136‑1 (MQ=255)
                                                                                                                           gTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataat              >  8:24777/1‑136 (MQ=255)
                                                                                                                           gTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  4:299212/139‑1 (MQ=255)
                                                                                                                           gTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  6:183113/139‑1 (MQ=255)
                                                                                                                           gTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  4:138919/139‑1 (MQ=255)
                                                                                                                           gTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  5:282415/139‑1 (MQ=255)
                                                                                                                           gTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCGCGTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataat              <  8:298602/136‑1 (MQ=255)
                                                                                                                              tatttaCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATg         <  1:314137/138‑1 (MQ=255)
                                                                                                                                tttaCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGta      <  2:243417/139‑1 (MQ=255)
                                                                                                                                tataCAATC‑TTTTTTTATTAGCCGACAAATTTCTCCCTCATCTAGTCTCTTTATATCTTTATATTAACAAATAGGACTAATTCAACAGTCGCGCGGGACAGCTATATGAAAGGTATATTTTATTGTATAATTGAtagaa      >  5:205744/3‑135 (MQ=255)
                                                                                                                                  taCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  4:327441/96‑1 (MQ=255)
                                                                                                                                  taCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           >  4:248180/1‑132 (MQ=255)
                                                                                                                                  taCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGt       >  7:109287/1‑136 (MQ=255)
                                                                                                                                   aCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGt       <  7:327008/135‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAG  >  Exported/2042‑2313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑