Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,581,732	(T)6→7	intergenic (‑115/+39)	pstS ← / ← pbpA	phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,581,726	1	.	T	100.0%	198.3 / NA	54	intergenic (‑109/+45)	pstS/pbpA	phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (47/7);  total (47/7)

TTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCG‑TTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCT  >  BS168_reference_genome/2581610‑2581850                                                                                                                      |                                                                                                                              tttttCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGc                                                                                                          >  5:53303/1‑139 (MQ=255)                            tataATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑tttttttt                                                                                 >  7:274741/1‑136 (MQ=255)                                 tgtgTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTCAACCGAATGTAAAGGTTTTGTAAATCAGTGCGTTTTTTTGTAAAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATc                                                                         <  2:284998/139‑1 (MQ=255)                                    gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                       >  5:33943/1‑138 (MQ=255)                                    gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                       >  2:184826/1‑138 (MQ=255)                                    gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                       >  6:44345/1‑138 (MQ=255)                                    gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                       >  6:186947/1‑138 (MQ=255)                                     tAATTTGTCCTACCCGTATAGAGAAAAGGTTCTATTTAAAACGGGTTTGAACCGAATGTAACGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCaga                                                                      <  3:133629/138‑1 (MQ=255)                                       aTTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                       >  8:291171/1‑135 (MQ=255)                                        tttGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                       >  7:193181/1‑134 (MQ=255)                                           gTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTTGTTTTGAGAAGCCGtt                                                               >  5:86251/1‑139 (MQ=255)                                                      ataGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTg                                                     >  2:95769/1‑138 (MQ=255)                                                         gagTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑‑TTTTTTTTATTTTTAACAATACGTTGTTTTTTTAGCAttt                                                >  2:197371/1‑139 (MQ=255)                                                            tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTATTTTTCAGAAGCAGTTGTTGTTTCTGCAGTttc                                              >  5:254252/1‑139 (MQ=255)                                                            tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCAtttta                                              >  6:316873/1‑138 (MQ=255)                                                            tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc                                              >  6:291530/1‑139 (MQ=255)                                                            tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc                                              >  2:91410/1‑139 (MQ=255)                                                            tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc                                              >  2:230588/1‑139 (MQ=255)                                                            tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCAACATCATGTGAGCG‑TTTTTTTTTAGTTATAAGAAGACGTTGTGTTTTCTGCAgttta                                              >  2:101169/1‑138 (MQ=255)                                                            tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCAAAATCATTTGCGCT‑TTTTTTTTTAGTTATCATAATCCGTTTTGTTTTGTGATtttta                                              >  4:79787/1‑138 (MQ=255)                                                              aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGTTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                             >  6:83361/1‑138 (MQ=255)                                                              aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                             >  6:1892/1‑139 (MQ=255)                                                              aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                            >  1:85605/1‑139 (MQ=255)                                                               aGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGAAGTTGTGTTTTCTGCATTttctt                                            >  5:242376/1‑138 (MQ=255)                                                                 gTTCTATGTAAAAAGTGTTAGAACCGAAAGTTAAGGAATTCTAAATCAGTGCGTTTTTTTGAATAAAAAAAACGCCCACAAAATGTGAGAG‑TATATTTTTAGTTATCACAAGACGTTCAGTTTTCTGCATTttatt                                            >  8:24686/1‑136 (MQ=255)                                                                 gTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                             >  8:266279/1‑135 (MQ=255)                                                                    cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTATTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                      >  5:223641/1‑139 (MQ=255)                                                                    cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                      >  5:181741/1‑139 (MQ=255)                                                                      aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                    >  1:360183/1‑139 (MQ=255)                                                                      aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                    >  6:176373/1‑139 (MQ=255)                                                                      aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                    >  4:272549/1‑139 (MQ=255)                                                                       tGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                      >  8:131391/1‑136 (MQ=255)                                                                       tGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                      >  7:181457/1‑136 (MQ=255)                                                                       tGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAt                                        <  8:274741/134‑1 (MQ=255)                                                                           aaaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCATTTTTGTCtt                                >  3:79042/1‑138 (MQ=255)                                                                            aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCagaag                                                                    >  4:305094/1‑101 (MQ=255)                                                                            aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCagaag                                                                    <  3:305094/101‑1 (MQ=255)                                                                            aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTa                              <  5:176373/139‑1 (MQ=255)                                                                            aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGATTTCTGCATTTTCTTCATTTTTGTCTTTa                              >  2:347875/1‑139 (MQ=255)                                                                                 gTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCtt                         >  6:96480/1‑139 (MQ=255)                                                                                 gTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCtt                         >  2:132029/1‑139 (MQ=255)                                                                                 gTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCtt                         >  5:121410/1‑139 (MQ=255)                                                                                 gTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGGTGTGTTGTCTGCATGTTCTTCATTTTTGGCTTTATTCtt                         >  2:77946/1‑139 (MQ=255)                                                                                 gTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGAAGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCtt                         >  3:368230/1‑139 (MQ=255)                                                                                      aaCCGAATGTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCtttt                       >  7:129530/1‑136 (MQ=255)                                                                                              gTTAAGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTa               >  7:239176/1‑136 (MQ=255)                                                                                                  aGGTTTTGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACct            <  8:129530/135‑1 (MQ=255)                                                                                                       ttGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTGTTTTGCTGAGTACCTGCTTTGTcc   >  3:296632/1‑139 (MQ=255)                                                                                                       ttGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCttt       <  8:181457/135‑1 (MQ=255)                                                                                                       ttGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTcc   >  6:257302/1‑139 (MQ=255)                                                                                                        tGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGGTGAGTACGTTCTTTGTCCt  >  5:306570/1‑139 (MQ=255)                                                                                                        tGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCt  >  2:85476/1‑139 (MQ=255)                                                                                                        tGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTATGCATTTTCTTCAATTTTGTCTTTATTATTTTGCTGAGTACCTTCTTTgctgt  >  3:32406/1‑135 (MQ=255)                                                                                                        tGTAAATCAGTGCGTTTTTTTGTATAAAAAAAACGCCCACATCATGGGAGCG‑TTTTTTTTTTGTTATCAGAAGACGTTGTGTTTTATGCATTTTCTTCAATTGTGTGGTTATTCTTTTGGTGGGTACCTTCtgtgggct  >  5:237914/1‑135 (MQ=255)                                                                                                                      |                                                                                                                              TTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGCG‑TTTTTTGTATAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCT  >  BS168_reference_genome/2581610‑2581850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑