Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	3,770,066	(A)8→9	intergenic (‑258/+38)	ywzF ← / ← csbD	conserved protein of unknown function/stress response protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,770,058	1	.	A	95.5%	223.8 / 2.1	67	intergenic (‑250/+46)	ywzF/csbD	conserved protein of unknown function/stress response protein
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base A (41/23);  total (43/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑AAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCC  >  BS168_reference_genome/3769926‑3770188                                                                                                                                        |                                                                                                                                   gTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aaaaa                                                                                                                               >  1:278020/1‑138 (MQ=255) gTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aaaaa                                                                                                                               >  1:330971/1‑138 (MQ=255)            ttACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑AAAAAAAAGACAAGaaa                                                                                                                   >  3:22155/1‑139 (MQ=255)              aCAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAg                                                                                                                      >  7:20212/1‑135 (MQ=255)                     aTGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGt                                                                                                           >  5:183298/1‑139 (MQ=255)                         catcatTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTc                                                                                                        <  3:19195/138‑1 (MQ=255)                          atcatTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCt                                                                                                      >  4:126591/1‑139 (MQ=255)                          atcatTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTC‑TTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTg                                                                                                     >  6:258628/1‑139 (MQ=255)                           tcatTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTg                                                                                                     <  3:96925/139‑1 (MQ=255)                             atTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGcc                                                                                                   >  2:83756/1‑139 (MQ=255)                               tatataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAACCATTTCGTTTCCTGCctc                                                                                                 >  2:38653/1‑139 (MQ=255)                               tatataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTcc                                                                                                       >  8:152345/1‑133 (MQ=255)                               tatataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTcc                                                                                                       <  7:152345/133‑1 (MQ=255)                                atataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGc                                                                                                    <  8:20212/135‑1 (MQ=255)                                     gaagaGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCg                                                                                            >  1:271242/1‑138 (MQ=255)                                     gaagaGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGt                                                                                           <  3:126591/139‑1 (MQ=255)                                      aagaGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTa                                                                                          >  1:217835/1‑139 (MQ=255)                                      aagaGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTa                                                                                          >  2:60548/1‑139 (MQ=255)                                      aagaGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATATCGTTTCCTGCCTCTTCCGTa                                                                                          >  2:167479/1‑139 (MQ=255)                                        gagGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTAc                                                                                         >  6:342569/1‑138 (MQ=255)                                         agGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTATTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGa                                                                                       >  5:277000/1‑139 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGATAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAt                                                                                      >  4:170175/1‑138 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGt                                                                                           >  1:92239/1‑133 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGt                                                                                           <  2:92239/133‑1 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAt                                                                                      >  6:250974/1‑138 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAt                                                                                      >  2:294301/1‑138 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAt                                                                                      >  5:211569/1‑138 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAt                                                                                      >  3:181368/1‑138 (MQ=255)                                           gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAt                                                                                      >  1:84266/1‑138 (MQ=255)                                            ccTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGa                                                                                       >  8:107828/1‑136 (MQ=255)                                            ccTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAtt                                                                                     >  1:104979/1‑138 (MQ=255)                                              tATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAt                                                                                      >  7:119752/1‑135 (MQ=255)                                                ttAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCt                                                                                >  1:262467/1‑139 (MQ=255)                                                   aTTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCg                                                                                            <  3:296466/124‑1 (MQ=255)                                                   aTTCAAAGAATATTTAAAAAATTTAGTTACCTTGACAAAATCAGATTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GCTA‑AAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCg                                                                                            >  4:296466/1‑124 (MQ=255)                                                     tCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATc                                                                           >  4:242585/1‑139 (MQ=255)                                                     tCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATc                                                                           >  1:172251/1‑139 (MQ=255)                                                      cAAAGAATATTTAAAAAATTTAGTTACCTTGACAAAATCAGCTTCTTTTTTTTTTATATTTATTCAAATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGATTCCTCCCACTTCCCTACGATTAATATTTATCt                                                                          >  2:190988/1‑139 (MQ=255)                                                        aaGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGa                                                                        <  2:217835/139‑1 (MQ=255)                                                             tatTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTATTCCGTACGATTAATCTTTATCTGAAAAtt                                                                   >  6:134084/1‑139 (MQ=255)                                                              atTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAAtt                                                                   >  3:138398/1‑138 (MQ=255)                                                               tttAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTa                                                                 >  1:44604/1‑139 (MQ=255)                                                                        tttAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCt                                                        <  3:35367/139‑1 (MQ=255)                                                                        tttAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCt                                                        <  3:242585/139‑1 (MQ=255)                                                                        attacttacCATGACCAAAACATCTTCCTTTTTTGTTATATTTATTCACATCAGAAAAACCC‑A‑AAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCt                                                        <  3:282268/138‑1 (MQ=255)                                                                                ccTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGat                                                <  5:342569/139‑1 (MQ=255)                                                                                 cTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCg                                                  <  7:38185/136‑1 (MQ=255)                                                                                  ttGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGatat                                              >  5:382684/1‑139 (MQ=255)                                                                                          aTCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTg                                       >  6:66790/1‑138 (MQ=255)                                                                                          aTCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTg                                       >  3:139855/1‑138 (MQ=255)                                                                                             aGCTTC‑TTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGAttt                                  <  5:258628/139‑1 (MQ=255)                                                                                              gCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGAttt                                  <  2:205002/139‑1 (MQ=255)                                                                                               cttcttTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTTCCTCTTCCGTACGATTAATCTTTATCt                                                                          <  3:79478/98‑1 (MQ=255)                                                                                               cttcttTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCt                                                                          >  4:79478/1‑98 (MQ=255)                                                                                                  cttTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTc                                 >  7:343434/1‑136 (MQ=255)                                                                                                     tttttGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATACTTGGCTTTCACGATATCCTTTTGGATTTCGcc                              >  8:187853/1‑136 (MQ=255)                                                                                                      ttttGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCttt                           <  6:183298/138‑1 (MQ=255)                                                                                                           ttATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCtt                     <  6:277000/139‑1 (MQ=255)                                                                                                             atatTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGATAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTGTTGCCTTAt                   >  5:381279/1‑139 (MQ=255)                                                                                                             atatTTATTCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCGTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTa                    <  1:190988/138‑1 (MQ=255)                                                                                                                   attCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTGATCttttt             >  4:284992/1‑139 (MQ=255)                                                                                                                    ttCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCttttt             <  2:104979/138‑1 (MQ=255)                                                                                                                    ttCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCttttt             <  2:262467/138‑1 (MQ=255)                                                                                                                     tCACATCAGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTg           >  3:89975/1‑139 (MQ=255)                                                                                                                            aGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGc          >  7:241015/1‑133 (MQ=255)                                                                                                                            aGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGc          <  8:241015/133‑1 (MQ=255)                                                                                                                            aGAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCATTTTGCCTTATCTTTTTTGCCtt       >  8:273373/1‑136 (MQ=255)                                                                                                                             gAAAAA‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGc   <  6:211569/139‑1 (MQ=255)                                                                                                                              aaaaa‑GC‑GAAAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGcc  <  2:330971/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑AAAAAAAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCC  >  BS168_reference_genome/3769926‑3770188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑