Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,826	+C	intergenic (+1/‑4)	trnI‑Asn → / → trnI‑Thr	tRNA‑Asn/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,825	1	.	C	97.7%	293.0 / ‑0.2	88	noncoding (72/72 nt)	trnI‑Asn	tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (47/39);  total (48/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑G‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCG  >  BS168_reference_genome/165690‑165954                                                                                                                                          |                                                                                                                                  gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑G‑ccatt                                                                                                                              <  4:330426/139‑5 (MQ=21)  tAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑G‑ccattg                                                                                                                             >  1:314732/1‑134 (MQ=21)     gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑G‑ccattg                                                                                                                             >  8:332871/1‑131 (MQ=21)        gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTATCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCa                                                                                                                        <  4:104916/139‑1 (MQ=21)          cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCATTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCa                                                                                                                        >  1:120254/1‑137 (MQ=21)          cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTCCCTGCGGA‑GCCATTGCTTCCATa                                                                                                                      <  2:138569/139‑1 (MQ=21)            ccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCAt                                                                                                                       <  7:209285/136‑1 (MQ=18)            ccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGc                                                                                                                    <  5:281825/139‑1 (MQ=21)            ccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGc                                                                                                                    <  6:354951/139‑1 (MQ=21)            ccAAGCAGATTTATTAATTCTACCATGTTTGCTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCGCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCAt                                                                                                                       <  7:21904/136‑1 (MQ=18)             cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCa                                                                                                                        >  8:365067/1‑134 (MQ=18)             cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCa                                                                                                                        >  8:234374/1‑134 (MQ=18)             cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGA‑GCCATTGCTTCCa                                                                                                                        >  7:251502/1‑134 (MQ=18)               aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCt                                                                                                                   >  6:116787/1‑137 (MQ=21)                 cAGTTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTcagc                                                                                                               <  4:323779/139‑1 (MQ=18)                    aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAgg                                                                                                            <  3:195521/139‑1 (MQ=21)                         ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       <  1:181152/139‑1 (MQ=21)                         ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       <  3:132751/139‑1 (MQ=21)                         ttaATTCTACCATGGTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       <  6:160938/139‑1 (MQ=18)                              tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                  >  6:287647/1‑139 (MQ=21)                                  ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                 <  2:106105/136‑1 (MQ=18)                                       tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa                                                                                          <  3:210722/138‑1 (MQ=21)                                            ttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                     <  5:287647/138‑1 (MQ=21)                                             tATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAgg                                                                                       >  8:297070/1‑135 (MQ=18)                                               tGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTc                                                                                                                  >  7:234513/1‑106 (MQ=14)                                               tGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTc                                                                                                                  <  8:234513/106‑1 (MQ=14)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       >  1:281352/1‑102 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       <  2:281352/102‑1 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                 <  2:336241/108‑1 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                 >  1:336241/1‑108 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                               >  5:247677/1‑110 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                               <  6:247677/110‑1 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  5:196347/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  4:281928/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  1:339358/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  6:270373/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  <  1:204724/139‑1 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  6:366750/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  6:68017/1‑139 (MQ=255)                                                                 tCCGGAGTAGCTCTGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  <  3:146634/138‑1 (MQ=255)                                                                   cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCa                                                                                                                        >  6:81650/1‑82 (MQ=9)                                                                   cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCa                                                                                                                        <  5:81650/82‑1 (MQ=9)                                                                   cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  7:131897/1‑136 (MQ=255)                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                     <  2:323072/115‑1 (MQ=255)                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                     >  1:323072/1‑115 (MQ=255)                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCtt                                                             >  4:331439/1‑139 (MQ=255)                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTAACTGCGGC‑GCCATTTATTCCATAGCTCTGCAGGTAGAGCACTTCCATGGTAAGTAAGAGGTCAGCGGTTCGAGCCCGCtg                                                             >  4:376375/1‑138 (MQ=21)                                                                       ggaGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGATCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           <  1:150685/137‑1 (MQ=255)                                                                        tAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCtt                                                             >  7:81002/1‑136 (MQ=255)                                                                        tAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           >  2:168039/1‑138 (MQ=255)                                                                          gcgcAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCTTGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAg                                                        <  3:63855/136‑1 (MQ=37)                                                                          gCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAg                                                        >  4:140445/1‑139 (MQ=255)                                                                             cAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAg                                                        <  7:234374/136‑1 (MQ=255)                                                                              aGTGGTAGAGCTAGCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGGTCGAGCCCGCTTGGAAg                                                        <  7:12381/135‑1 (MQ=255)                                                                               gTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCt                                                      <  8:206762/136‑1 (MQ=255)                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaa                                                   <  2:219562/138‑1 (MQ=255)                                                                                tGGTAGAGCGATCGGCTGTTAACCGATCGGGCGTAGGGTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                  <  2:240568/139‑1 (MQ=37)                                                                                cggTAGAGCTATCGGCTGTTAACCAATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                  <  5:22274/138‑1 (MQ=255)                                                                                  gTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAAt                                                 <  5:270373/138‑1 (MQ=255)                                                                                     gagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGta                                              <  6:240332/138‑1 (MQ=255)                                                                                     gagCTATCGGCTGTAAACCGATCGGTCGAAGCATCGAATCCTACCTGCGCACC‑CATAGCTTCCATACCTCAGCAGGTAGAGCACATCCATGGTAAGGAAGACGTCAGCGGTTCGAGCCCGCTTGGAACCTTAAATGtat                                             >  2:171263/1‑139 (MQ=255)                                                                                      agCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           >  1:184493/1‑124 (MQ=255)                                                                                      agCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           <  2:184493/124‑1 (MQ=255)                                                                                        cTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtattat                                          >  6:98374/1‑139 (MQ=255)                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAATAGGTCAGCGGTTCTCGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  4:81918/1‑139 (MQ=37)                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  6:258581/1‑139 (MQ=255)                                                                                           tCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtattat                                          >  7:43309/1‑136 (MQ=255)                                                                                           tCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTAc                                       >  4:91628/1‑139 (MQ=255)                                                                                             ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTattatt                                         >  8:180644/1‑135 (MQ=255)                                                                                             ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  7:176892/1‑136 (MQ=255)                                                                                             ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  8:268734/1‑136 (MQ=255)                                                                                             ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  7:30969/1‑136 (MQ=255)                                                                                             ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        <  7:297070/136‑1 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCaa                                    <  7:332871/135‑1 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGtt                                >  3:278785/1‑139 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGtt                                >  4:258338/1‑139 (MQ=255)                                                                                                     aCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGACGCTTAAATGTATTATTACCAAGGtt                                >  8:140125/1‑136 (MQ=255)                                                                                                     aCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCACGCTTGGAAGCTGAAATGAATTATTACCATGGtt                                >  7:370911/1‑136 (MQ=38)                                                                                                       cGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCa                           >  5:48265/1‑139 (MQ=255)                                                                                                         aTCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                          >  6:331488/1‑138 (MQ=255)                                                                                                         aTCGGTCGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                          >  1:206960/1‑138 (MQ=255)                                                                                                               cGTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGCGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAg                       >  8:183935/1‑135 (MQ=255)                                                                                                                gTAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAgg                      <  7:268734/135‑1 (MQ=255)                                                                                                                 tAGGTTCGAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                   >  2:215068/1‑137 (MQ=255)                                                                                                                        gAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttt          >  3:118353/1‑139 (MQ=255)                                                                                                                        gAATCCTACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttt          >  2:367891/1‑139 (MQ=255)                                                                                                                           tcgtACCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTGCTTGGTAAGGATGAGGGCAGCGGTGCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTa       <  2:314732/136‑1 (MQ=255)                                                                                                                               aCCTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGATAGC‑TTTTTTATTGc   >  3:336245/1‑138 (MQ=255)                                                                                                                                ccTGCGGA‑GCCATTGCTTCCATAGCTCAGCATGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg  <  3:81918/139‑1 (MQ=255)                                                                                                                                ccTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg  <  3:281928/139‑1 (MQ=255)                                                                                                                                ccTGCGGA‑GCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg  <  5:331488/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGA‑G‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCG  >  BS168_reference_genome/165690‑165954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑