Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	BS168_reference_genome	2,344,013	Δ39 bp	coding (2570‑2608/2745 nt)	ponA →	peptidoglycan glycosyltransferase (penicillin‑binding proteins 1A and 1B)

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	BS168_reference_genome	2344013	2344051	39	50 [0]	[0] 50	ponA	peptidoglycan glycosyltransferase (penicillin‑binding proteins 1A and 1B)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	BS168_reference_genome	= 2344012	0 (0.000)	50 (0.680)	35/234	0.6	100%	coding (2569/2745 nt)	ponA	peptidoglycan glycosyltransferase (penicillin‑binding proteins 1A and 1B)
	?	BS168_reference_genome	2344052 =	0 (0.000)					coding (2609/2745 nt)	ponA	peptidoglycan glycosyltransferase (penicillin‑binding proteins 1A and 1B)

AGATGATGAGAAGACTCAGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  BS168_reference_genome/2343870‑2344012 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgacaacaGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCATCATCCATTGAAAAAA  >  BS168_reference_genome/2344052‑2344180                                                                                                                                                                                                                                                                                  AGATGATCAGAAGACGCAGGATGATACACAAACTGATGATTCTCAAAAAGAGGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGAC                                                                                                                                       <  4:134692/139‑1   ATGATGAGAAGACTCAGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAA                                                                                                                                     <  4:286151/139‑1       TGAGAAGACTCAGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGT                                                                                                                                 <  6:155936/139‑1          GAAGACTCAGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAA                                                                                                                               <  6:15522/138‑1             cACTCAGGATTATACAAAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAAC                                                                                                                           <  6:83044/138‑1             GACTCAGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAAC                                                                                                                           <  1:262493/139‑1                  AGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATTATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGA                                                                                                                                        <  1:298419/121‑1                  AGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGA                                                                                                                                        >  2:298419/1‑121                   GGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGA                                                                                                                      >  4:328410/1‑138                   GGATGACACAAAAACTGATGATCCCCAAAAAGAGGACCGTCACACGGATCAAGATCAGACAGATGATTCAACAAACGATCAACACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGAT                                                                                                                     <  1:115132/139‑1                      aGATACACAAACTGATCATTCTCAAAAAGTTGACGGGCAGCCGGATCAAGATCAGACAGAGGATTCAACAAACGAACAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACG                                                                                                                  <  3:260539/138‑1                              AAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGG                                                                                                          >  4:354595/1‑139                              AAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGG                                                                                                          >  2:31266/1‑139                              AAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGT                                                                                                                                 <  6:130533/116‑1                              AAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGT                                                                                                                                 >  5:130533/1‑116                                ACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGCTCAAGCCAAAAAACAAGACAATACGAACACCAATCCGTCCGCCAACAGTACCAACCAGGATACGTCGGATGGT                                                                                                         >  6:26091/1‑138                                ACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCACTCCGTCCGACAACAGTAACAACCCGGATCCGTCGGATGGT                                                                                                         >  6:20996/1‑138                                ACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGT                                                                                                         >  5:91748/1‑138                                       ATTCTCAAAAAGATGACGGTCAGACTGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAACCAAGACAATACGAACACCAATCCGTCCTACAACAGTAACAACCAGGATACGTCGGATGGTGATTC                                                                                                    >  7:125887/1‑136                                          CTCAAAAAGATGCCGGGCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGA                                                                                                  <  8:273054/135‑1                                          CTCAAAAAGATGACGGTCAGACTGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGA                                                                                                  <  7:124264/135‑1                                          CTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACT                                                                                               <  3:336197/138‑1                                            CAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTG                                                                                            >  5:356831/1‑139                                              AAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCT                                                                                             >  8:246402/1‑136                                              AAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCT                                                                                             <  7:246402/136‑1                                              AAAAGATGACGGGCAGACGGAGCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCT                                                                                             <  7:148761/136‑1                                                            gactgcATCAAGATCAGACTGATGATTCAACAAACGATCAATAAAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAAC                                                                            <  4:327709/133‑1                                                            AGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAAC                                                                            <  5:81961/139‑1                                                               CGGATCAAGAACAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGACGGATGGTGATTCGAACTCTGGAAACAATGATTCAA                                                                             >  8:127166/1‑135                                                                  ATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTC                                                                      >  3:210536/1‑139                                                                      AGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGA                                                                   >  4:363519/1‑138                                                                      AGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAA                                                                                        <  8:1943/117‑1                                                                      AGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAA                                                                                        >  7:1943/1‑117                                                                       GATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGTATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACA                                                                 <  3:354595/139‑1                                                                          CAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACG                                                               >  4:252926/1‑138                                                                            GACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCACTCCGTCCGCCAACAGTAACAACCAGGATACGTCGGATGGTGATTCGACCTCTGGTAAGAATGATTCAACAGGTTCTGACACGAA                                                             >  6:74906/1‑138                                                                            GACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACtgtc                                                            >  3:327709/1‑135                                                                            GACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAA                                                             >  1:89850/1‑138                                                                                 ATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATA                                                           >  7:105003/1‑135                                                                                    ATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAA                                                        >  7:31054/1‑135                                                                                          CAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGA                                              <  3:252926/139‑1                                                                                           AAACGATCAAGACAAAAAACAAGACAATACGACCACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGAC                                             <  3:292029/139‑1                                                                                                     GACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACGAGTATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTA                                      <  8:105003/136‑1                                                                                                     GACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAAT                                    >  4:373244/1‑138                                                                                                              CAAGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACAC                             <  8:31054/136‑1                                                                                                                AGACAATACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAAC                         >  1:120533/1‑138                                                                                                                      TACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCA                  >  4:322498/1‑139                                                                                                                      TACGAACACCAATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCA                  >  3:129065/1‑139                                                                                                                                AATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCATCATCCATT         >  2:29693/1‑138                                                                                                                                 ATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCATCATCCATTG        >  4:101948/1‑138                                                                                                                                 ATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCATCATCCATTG        >  3:80415/1‑138                                                                                                                                 ATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGT                                                                                          >  6:332723/1‑56                                                                                                                                 ATCCGTCCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGT                                                                                          <  5:332723/56‑1                                                                                                                                       CCGACAACAGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCATCATCCATTGAAAAAA  >  1:331216/1‑138                                                                                                                                                                                                                                                                                  AGATGATGAGAAGACTCAGGATGATACACAAACTGATGATTCTCAAAAAGATGACGGTCAGACGGATCAAGATCAGACAGATGATTCAACAAACGATCAAGACAAAAAACAAGACAATACGAACACCAATCCGTCCGACAACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  BS168_reference_genome/2343870‑2344012 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgacaacaGTAACAACCAGGATACGTCGGATGGTGATTCGAACTCTGGTAAGAATGATTCAACAGGTTCTGACACGAATAAAAATAAAACAGACACATCTAATAAAACACAAACAAACTCATCATCCATTGAAAAAA  >  BS168_reference_genome/2344052‑2344180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.