| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | BS168_reference_genome | 892,022 | A→T | N196I (AAT→ATT) | malR → | transcriptional activator of the Mal operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 892,022 | 0 | A | T | 96.8% | 212.7 / ‑3.8 | 63 | N196I (AAT→ATT) | malR | transcriptional activator of the Mal operon |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (31/30); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCGCAT > BS168_reference_genome/891891‑892159 | taatttGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTaaa < 5:205867/138‑1 (MQ=255) tGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑Ca < 3:350299/138‑1 (MQ=255) tGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTCTCCGGAGAAATACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑Ca < 5:288625/138‑1 (MQ=255) tGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑Caa < 2:328450/136‑1 (MQ=255) tGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑Caa > 1:328450/1‑136 (MQ=255) cgcgATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATg < 8:341947/136‑1 (MQ=255) aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGAc < 2:105988/139‑1 (MQ=255) aGTGAACGATCTGTTTATCATCTTTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTg < 8:46811/136‑1 (MQ=255) gAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGCCTCCg > 3:260639/1‑139 (MQ=255) aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCg > 2:133015/1‑138 (MQ=255) aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTcc < 7:20430/136‑1 (MQ=255) aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCa < 4:29347/139‑1 (MQ=255) atcatcATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATg > 6:208672/1‑139 (MQ=255) atcatTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCg < 5:282581/138‑1 (MQ=255) tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg > 4:63363/1‑139 (MQ=255) tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg > 4:363032/1‑139 (MQ=255) ctctTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACaa < 8:196381/135‑1 (MQ=255) ctctTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg > 8:59154/1‑136 (MQ=255) tttCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCaaa < 5:47502/137‑1 (MQ=255) tCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACt > 1:12073/1‑108 (MQ=255) tCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACt < 2:12073/108‑1 (MQ=255) tCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg > 1:310853/1‑139 (MQ=255) tCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTACCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCt < 6:56745/138‑1 (MQ=255) ccgcctaaaCACCGCTGCTGATTCCTCAGGCAATAATCCTTTATGAAAAGGGAATTCCCTTAATTTCCATAACCATTTTAAAAAA‑CAATG‑CCCCCCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAAcc < 7:369368/129‑1 (MQ=255) cacCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAg > 1:278059/1‑139 (MQ=255) cGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa < 2:211338/139‑1 (MQ=255) ggAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGAt < 3:181604/139‑1 (MQ=255) gAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa > 5:307553/1‑137 (MQ=255) gCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGa < 6:307553/139‑1 (MQ=255) tCAGGCAAGAATGCTTTCTGCAAAGGGTATTCACTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCAGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAAtt > 4:200320/1‑139 (MQ=255) cAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTg > 3:364818/1‑139 (MQ=255) aGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt < 6:103284/139‑1 (MQ=255) aaTGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCtttt > 6:251062/1‑139 (MQ=255) cTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCa < 6:44934/138‑1 (MQ=255) tttCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGAc < 2:300238/67‑1 (MQ=255) tttCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGAc > 1:300238/1‑67 (MQ=255) ggTATTCCCTTTATTTCCATTACGAATTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCGTTTTCAACGTTTTTTCTCg > 2:143815/1‑139 (MQ=255) gTATTCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTtc > 8:146145/1‑135 (MQ=255) tCCCTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGGACCGTTTTTTCTCGTTGGc > 3:203785/1‑139 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 6:166335/138‑1 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCATTATGCGACAAGCAAACCTGTGACATAAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 4:79747/138‑1 (MQ=255) ttATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGATCGGAAATTGTCGCTTTTGCAACGTTTTTTATAGTTTGCGAAg > 6:201465/1‑138 (MQ=255) ttATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCa > 6:162551/1‑116 (MQ=255) ttATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCa < 5:162551/116‑1 (MQ=255) ttATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 2:141721/1‑138 (MQ=255) ttATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 1:222132/1‑138 (MQ=255) ttATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 4:310865/1‑138 (MQ=255) ttATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 5:92611/1‑138 (MQ=255) ctATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAg < 6:295734/87‑1 (MQ=255) ctATTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAg > 5:295734/2‑88 (MQ=255) aTTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCa < 3:63363/138‑1 (MQ=255) aTTTCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCAt > 6:194523/1‑139 (MQ=255) tttCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 7:83696/1‑135 (MQ=255) tttCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 8:67893/1‑135 (MQ=255) tttCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 7:253476/1‑135 (MQ=255) tttCCATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTAGTTGGCGAAg > 7:33834/1‑135 (MQ=255) cATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTTTGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTc < 2:278059/139‑1 (MQ=255) cATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGtt < 4:364818/138‑1 (MQ=255) cATTACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTc < 2:222132/139‑1 (MQ=255) ttACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg > 6:4267/1‑139 (MQ=255) ttACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg < 5:201465/139‑1 (MQ=255) ttACGAATTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg < 1:143815/139‑1 (MQ=255) tACGATTTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTc > 7:369199/1‑136 (MQ=255) ctaTTTAAAAAACCAATGGCCGGGCCCCGCTGCCTCCCCAAAAGC‑TTATGCGACAAGCAAACCTGTGACATTAAGCGAT‑GAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCGCat < 6:25909/137‑1 (MQ=255) | TAATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAA‑CAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCGCAT > BS168_reference_genome/891891‑892159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |