Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 1,224,521 A→G E356G (GAA→GGA)  oppD → oligopeptide ABC transporter (ATP‑binding protein)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,224,5210AG98.0% 178.2 / NA 51E356G (GAA→GGA) oppDoligopeptide ABC transporter (ATP‑binding protein)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (25/25);  minor base T (0/1);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTATCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGAAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGAAACATTAGGGCTGGT  >  BS168_reference_genome/1224389‑1224654
                                                                                                                                    |                                                                                                                                     
aGGTATCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAgaggag                                                                                                                                     <  8:161107/135‑1 (MQ=255)
aGGTATCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtg                                                                                                                                  >  1:267643/1‑138 (MQ=255)
    aTCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatg                                                                                                                              >  6:42580/1‑138 (MQ=255)
     tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatgaa                                                                                                                            >  3:331166/1‑139 (MQ=255)
     tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatgaa                                                                                                                            >  2:329605/1‑139 (MQ=255)
     tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatgaa                                                                                                                            >  2:145153/1‑139 (MQ=255)
     tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCGGAAGCGGTAAAAGCTAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGCTAGGAGTtgaaggaa                                                                                                                            >  5:296438/1‑139 (MQ=255)
        gATACTCTTTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatgaa                                                                                                                            >  8:256513/1‑136 (MQ=255)
         aTACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTgaatgaat                                                                                                                           >  7:271592/1‑136 (MQ=255)
         aTACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTgaatgaat                                                                                                                           >  7:154849/1‑136 (MQ=255)
             tCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTg                                                                                                                     >  4:168011/1‑138 (MQ=255)
               aTTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGttgaatgaattgaatgaa                                                                                                                   >  4:327606/1‑138 (MQ=255)
                         aTCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGa                                                                                                                    >  2:89465/1‑127 (MQ=255)
                         aTCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGa                                                                                                                    <  1:89465/127‑1 (MQ=255)
                             tGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAAt                                                                                                     >  6:137518/1‑138 (MQ=255)
                               gctgctTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCaa                                                                                                  <  5:137518/139‑1 (MQ=255)
                               gctgctTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCaa                                                                                                  <  4:43749/139‑1 (MQ=255)
                               gctgctTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCaa                                                                                                  <  4:334617/139‑1 (MQ=255)
                                ctgctTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAAt                                                                                                     >  7:32247/1‑135 (MQ=255)
                                ctgctTCATCCTGACGCGCCAAAGGTAGAGCCGCCTAAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAAt                                                                                                     >  7:369383/1‑135 (MQ=255)
                                      cATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACAtt                                                                                             <  2:127496/137‑1 (MQ=255)
                                                           gagCCGCCGGAACCGGGAAAAGCGCAAAAGCCTAAACTGGCAAACACGTTTGCACACCCGGTCTTAGTGAGAGGAGTTGAATGCATTGACTGAAAAACTATTAGCAATCAAACATTGAAAACAGCCCTTTGTCACGcc                                                                       >  4:19472/1‑138 (MQ=255)
                                                                 ccTGTAGCGGTACAAGCGAAAATGAGGACACTTGCCAACACGTGTGAAAACCATGTCTTATTGAGATTCGGGTCATGAATTGACTTCAAAAATATTCGCAATCACCCATTTAAACAAGAACTTTGTCACGCCGAGGGGa                                                                <  2:365091/139‑1 (MQ=255)
                                                                 ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTaaaa                                                                                        <  2:3236/115‑1 (MQ=255)
                                                                 ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTaaaa                                                                                        >  1:3236/1‑115 (MQ=255)
                                                                 ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAgggg                                                                 >  5:93417/1‑138 (MQ=255)
                                                                 ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAgggg                                                                 >  3:197194/1‑138 (MQ=255)
                                                                 ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                <  3:327606/139‑1 (MQ=255)
                                                                 ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                <  5:42580/139‑1 (MQ=255)
                                                                 ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                >  4:84784/1‑139 (MQ=255)
                                                                    gAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAgggg                                                                 >  7:293009/1‑135 (MQ=255)
                                                                       tcGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATTAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACg                                                             <  8:98783/135‑1 (MQ=255)
                                                                       gCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGtt                                                          <  1:145153/139‑1 (MQ=255)
                                                                       gCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGtt                                                          <  1:329605/139‑1 (MQ=255)
                                                                           tAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGtt                                                          >  4:304106/1‑135 (MQ=255)
                                                                           tAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGtt                                                          <  3:304106/135‑1 (MQ=255)
                                                                           tAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAgg                                                      >  2:9241/1‑139 (MQ=255)
                                                                                         cGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAgatgat                                           <  8:369383/136‑1 (MQ=255)
                                                                                               cTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCAttt                                  <  4:197194/139‑1 (MQ=255)
                                                                                                  gaaaaCACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCAttt                                  <  8:271592/134‑1 (MQ=255)
                                                                                                  gCAACCACGTTTGAAAACCCTGTCTTACTGAGAGGAGTTCAATGAATTGACTGAAAAACTAATAGAAATCAAACATTTAAAACAGCACTTTGACACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGat                               <  4:372379/139‑1 (MQ=255)
                                                                                                  gCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTTAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCAttt                                  <  8:154849/136‑1 (MQ=255)
                                                                                                  gCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCAttt                                  <  7:233024/136‑1 (MQ=255)
                                                                                                  gCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCAttt                                  <  8:60990/136‑1 (MQ=255)
                                                                                                  gCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGat                               <  2:5853/139‑1 (MQ=255)
                                                                                                  gCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGa                                >  1:222071/1‑138 (MQ=255)
                                                                                                  gCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGa                                >  5:380133/1‑138 (MQ=255)
                                                                                                  gCAAACACGGTTGAAAAACCTGTCTTATTGAGACGCGTAGAATGAATTGACTCAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGACACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGat                               <  2:183671/139‑1 (MQ=255)
                                                                                                                aaaaCCTGTCTTAGTGTGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCTTTTGATATCTATAAAGGTGa                 <  3:84784/139‑1 (MQ=255)
                                                                                                                aaaaCCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAATAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAgg                    <  7:256513/136‑1 (MQ=255)
                                                                                                                aaaaCCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGa                 <  3:168011/139‑1 (MQ=255)
                                                                                                                  aaCCTGTCTTAGTGATAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGaaa               <  6:380133/139‑1 (MQ=255)
                                                                                                                                agaggAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGAAACATTAGGGCtggt  <  6:93417/138‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AGGTATCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGAAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGAAACATTAGGGCTGGT  >  BS168_reference_genome/1224389‑1224654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑