Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,349,495	G→T	A325A (GCC→GCA)	pitB ←	putative low‑affinity inorganic phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,349,495	0	G	T	100.0%	288.8 / NA	81	A325A (GCC→GCA)	pitB	putative low‑affinity inorganic phosphate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/48);  total (33/48)

CCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGT  >  BS168_reference_genome/1349368‑1349628                                                                                                                                |                                                                                                                                      ccTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCAcc                                                                                                                            <  1:21314/139‑1 (MQ=255)    cacaTCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAg                                                                                                                         >  1:258195/1‑139 (MQ=255)          agAAATAGGTTCTGCTTTTTGTATTCGATTGTTCCGGATGGTTTTCCTGTCCCATCAAAAAAACCGCTCCTTTGACAGAACCGGTGTGACATTAAAAAATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCg                                                                                                                    <  2:124225/137‑1 (MQ=255)           gAAAGAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt                                                                                                                  >  6:191594/1‑139 (MQ=255)              aTAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt                                                                                                                  >  8:44141/1‑136 (MQ=255)                 ggTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt                                                                                                                  >  1:197858/1‑133 (MQ=255)                 ggTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt                                                                                                                  <  2:197858/133‑1 (MQ=255)                 ggTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAAt                                                                                                             <  2:32369/138‑1 (MQ=255)                 ggTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATc                                                                                                            >  4:11873/1‑139 (MQ=255)                     cTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                        <  4:324492/139‑1 (MQ=255)                      tGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTg                                                                                                                 >  1:289726/1‑129 (MQ=255)                        cTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                        <  2:199159/136‑1 (MQ=255)                        cTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                        <  4:13988/136‑1 (MQ=255)                        cTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                        <  6:152866/136‑1 (MQ=255)                        cTTATTGTAATCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                        <  4:240771/136‑1 (MQ=255)                           aTTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCg                                                                                                                                    >  5:159868/1‑105 (MQ=255)                           aTTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCg                                                                                                                                    <  6:159868/105‑1 (MQ=255)                           aTTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGt                                                                                                    <  2:222286/137‑1 (MQ=255)                           aTTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGt                                                                                                    <  4:205857/137‑1 (MQ=255)                            ttGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                                 <  2:258195/139‑1 (MQ=255)                            ttGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGt                                                                                                    <  8:241200/136‑1 (MQ=255)                            ttGTATTCGAGAGATCCGGATGGTTTTCCTGTTACATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTTTCGCTGAAATCGGAAGCGt                                                                                                    <  8:223911/136‑1 (MQ=255)                             tGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTTACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                                 <  3:298166/138‑1 (MQ=255)                             tGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                                 <  3:11873/138‑1 (MQ=255)                             tGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                                 <  5:37517/138‑1 (MQ=255)                              gTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgatg                                                                                                <  1:269369/138‑1 (MQ=255)                               tATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgatga                                                                                               <  4:137292/138‑1 (MQ=255)                               tATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtga                                                                                                  <  7:44141/135‑1 (MQ=255)                                 ttCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAccc                                                                                            <  1:155894/139‑1 (MQ=255)                                 ttCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAcc                                                                                             >  6:266271/1‑138 (MQ=255)                                  tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCAc                                                                                                                             >  3:62676/1‑105 (MQ=255)                                  tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCAc                                                                                                                             <  4:62676/105‑1 (MQ=255)                                  tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                        >  6:193695/1‑126 (MQ=255)                                  tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                        <  5:193695/126‑1 (MQ=255)                                  tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAccc                                                                                            >  1:330770/1‑138 (MQ=255)                                  tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCa                                                                                           >  5:29494/1‑139 (MQ=255)                                    gagagaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGcccc                                                                                            >  8:139203/1‑136 (MQ=255)                                     agagaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCa                                                                                           >  7:170693/1‑136 (MQ=255)                                     agagaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCa                                                                                           >  8:70630/1‑136 (MQ=255)                                     agagaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCGTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCTCTGAAATCGTCAGCGTGAGGCCCCa                                                                                           >  8:290199/1‑136 (MQ=255)                                      gagaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGt                                                                                        >  1:252090/1‑138 (MQ=255)                                        gaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAAt                                                                                     <  3:194409/139‑1 (MQ=255)                                             ggATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCa                                                                                <  2:289726/139‑1 (MQ=255)                                                   ttttCCTGTTCCATCTAAAAAAGCGCTCCTTTGTAAGAAGCGGTGTGACATTAAAATATCATTTTTAAAATAAAGTATGCGATGGCACCTAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCTTTCGCt                                                                          <  2:297149/139‑1 (MQ=255)                                                   ttttCCTGTTCCATCAAAAAAACCGCTCCTTTCACAGAAGCGGTGTGACATTAAAATAACATATTTAAAATAAAGTATGAGATGGCACCAAGTGACGCTCAAATCGGAAGCGAGATGACCCATGTAATGAGCATTCGCt                                                                          >  2:276964/1‑139 (MQ=255)                                                      tCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTTTCGCTGAAATCTGAAGCGTGATTAACCATTTAATTAGCATTCGCt                                                                          >  8:190632/1‑136 (MQ=255)                                                      tCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgatga                                                                                               <  5:209907/115‑1 (MQ=255)                                                      tCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgatga                                                                                               >  6:209907/1‑115 (MQ=255)                                                           ttCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCg                                                                    <  3:115605/137‑1 (MQ=255)                                                              cATCAAAAACACGGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGt                                                                   <  7:359610/135‑1 (MQ=255)                                                              cATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAgtg                                                                                                                       >  7:259352/1‑83 (MQ=255)                                                              cATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAgtg                                                                                                                       <  8:259352/83‑1 (MQ=255)                                                              cATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAccc                                                               <  5:191594/139‑1 (MQ=255)                                                                tCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATATAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCAAGTAATGAGCATTCGCTTGGCCGTAccc                                                               >  4:126622/1‑137 (MQ=255)                                                                tCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAccc                                                               >  4:132296/1‑137 (MQ=255)                                                                     aaaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTa                                                        <  2:330770/139‑1 (MQ=255)                                                                                  tGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAAGCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcg                                             <  3:306637/137‑1 (MQ=255)                                                                                  tGACAGAAGCGGTGTGACATTAAAATATCATAATTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCtttt                                                <  8:300935/134‑1 (MQ=255)                                                                                   gACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCTGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAc                                              <  7:190632/135‑1 (MQ=255)                                                                                    aCAGAAGCTGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcg                                             <  8:170693/135‑1 (MQ=255)                                                                                    aCAGAAGCGGTGTGACATTAAACTATCATATTTAAAATAAAGTATGCGATGGCACCAAGGGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGt                                         <  6:15626/139‑1 (MQ=255)                                                                                    aCAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcg                                             <  7:308231/135‑1 (MQ=255)                                                                                         aGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTa                                                        >  2:350632/1‑119 (MQ=255)                                                                                         aGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTa                                                        <  1:350632/119‑1 (MQ=255)                                                                                            ggTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGAc                                    >  7:32596/1‑136 (MQ=255)                                                                                             gtgtGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCAGTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGctc                                >  6:127710/1‑137 (MQ=255)                                                                                                tgACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCAACGa                             >  2:214860/1‑139 (MQ=255)                                                                                                  aCATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                                 >  4:381446/1‑69 (MQ=255)                                                                                                  aCATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                                 <  3:381446/69‑1 (MQ=255)                                                                                                   cATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcgcg                                           >  3:251276/1‑122 (MQ=255)                                                                                                   cATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcgcg                                           <  4:251276/122‑1 (MQ=255)                                                                                                           tatCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAg                      >  8:194371/1‑135 (MQ=255)                                                                                                              cATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATtgat               >  6:153868/1‑139 (MQ=255)                                                                                                              cATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCACTGAAATCGGAAGAGTGATGACCCATGTAATGAGCATTCGCATGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGACAAGAATtgat               >  5:131612/1‑139 (MQ=255)                                                                                                                   ttAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGt                                                           >  8:79969/1‑90 (MQ=255)                                                                                                                   ttAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGt                                                           <  7:79969/90‑1 (MQ=255)                                                                                                                     aaaaTAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATgaa            <  8:32596/135‑1 (MQ=255)                                                                                                                         tAAAGTATGCGATGGCACCAAGTGTCGCTGTAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCAAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAAt       <  8:259424/136‑1 (MQ=255)                                                                                                                         tAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTATTGAGCTTTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGGGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAc    <  1:276964/139‑1 (MQ=255)                                                                                                                         tAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAc    <  2:252090/139‑1 (MQ=255)                                                                                                                           aaGTATGCGATGGCACCAAGTGTCGCTGAAATCGGCAGCGTGATGACCCATTTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGt  <  2:172658/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                      CCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGT  >  BS168_reference_genome/1349368‑1349628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑