| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 166,344 | Δ1 bp | 100% | intergenic (+16/‑156) | trnI‑Ala → / → rrnH‑16S | tRNA‑Ala/ribosomal RNA‑16S |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 166,344 | 0 | G | . | 100.0% | 78.7 / NA | 44 | intergenic (+16/‑156) | trnI‑Ala/rrnH‑16S | tRNA‑Ala/ribosomal RNA‑16S |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (25/19); total (25/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAA > BS168_reference_genome/166210‑166466 | tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 1:106055/1‑135 (MQ=17)tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 5:82397/1‑135 (MQ=17)tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 7:196337/1‑135 (MQ=17)tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 7:52393/1‑135 (MQ=17)tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat < 7:7378/139‑5 (MQ=17)tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 8:7378/1‑135 (MQ=17)tGGGGTCGCAGGTTCGAATCCTGTCGTCCCGACCATTTTTTATGGGGACTTAGGTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTAAGCCGATCGATCCCGCTAGGCTGCACCAAAAATTTTTAAAAAAgatat > 6:228537/1‑135 (MQ=11) tCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCCCGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACt > 2:244919/1‑139 (MQ=12) cGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGaagaag < 6:82397/139‑1 (MQ=17) cGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGaagaag < 4:301844/139‑1 (MQ=17) gAATCCTGTCTTCCCGCCCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGt > 7:290428/1‑139 (MQ=12) aaTCCTGTCTTCCCGACCATTTTTTATGTGGCATTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg < 6:165162/139‑1 (MQ=255) ccTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCGCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACa < 2:125702/139‑1 (MQ=16) gTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTg > 1:30627/1‑139 (MQ=21) cccGACCATTTTTTATGGGGCCTTAGCTCATCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta < 8:23945/136‑1 (MQ=255) cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGCTCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATAc > 3:32897/1‑139 (MQ=255) cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGATGAAGTGACATTGTATAc > 2:254647/1‑139 (MQ=255) cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta < 4:216753/136‑1 (MQ=21) ccGACCATTTTTTATGGGGCATTAGCTAAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACAAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATAc > 7:199110/1‑138 (MQ=11) ttttttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTATAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAg > 6:44155/1‑139 (MQ=2) ttttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGt < 8:196337/138‑1 (MQ=11) gCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaa > 1:212459/1‑139 (MQ=11) gCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaa > 2:56097/1‑139 (MQ=11) ggAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaacaa > 7:277485/1‑138 (MQ=11) gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta > 3:11265/1‑97 (MQ=9) gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta < 4:11265/97‑1 (MQ=9) gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAt > 4:116803/1‑139 (MQ=11) gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAt > 8:275806/1‑139 (MQ=11) cgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATc > 1:132044/1‑139 (MQ=11) gcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCt > 6:124786/1‑139 (MQ=11) cTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaa < 1:164555/139‑1 (MQ=11) tGCACGCAGTAGGTCAGCGGTTCGATCCCTCTAGGCTACACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaaaa < 5:109928/138‑1 (MQ=11) tGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaaaa < 2:106055/138‑1 (MQ=11) tGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAAc > 5:293654/1‑139 (MQ=11) cGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTaaa < 2:218685/139‑1 (MQ=11) aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc > 6:46679/1‑137 (MQ=11) aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc > 2:285395/1‑137 (MQ=11) aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGAAATAGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTATGAAAACTAAAc > 7:46182/1‑137 (MQ=11) ggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACa < 2:132044/139‑1 (MQ=11) gTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACACCGCGGACAAACAAAATGATCTTTGAAAACTAACCAAGACa > 6:202004/1‑138 (MQ=11) aGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGCCATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAAc > 3:122994/1‑139 (MQ=2) aGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAAc > 3:94608/1‑139 (MQ=11) ggTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGTAAACTAAACAAGACAAAACGTa < 6:239336/139‑1 (MQ=2) ggTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTa < 5:44155/139‑1 (MQ=11) cGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAtt < 6:266973/139‑1 (MQ=11) cGCTAGGCTACACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAtt < 7:165452/139‑1 (MQ=2) cTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAtt > 7:236414/1‑137 (MQ=11) caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa > 7:156507/1‑138 (MQ=11) accaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaa > 4:86053/1‑138 (MQ=11) caAAAGTTTTTTAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaaaa < 3:46545/139‑1 (MQ=2) caAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaaaa < 8:277485/139‑1 (MQ=11) | TGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAA > BS168_reference_genome/166210‑166466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |