| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 238,265 | G→T | 21.1% | pseudogene (184/285 nt) | ybfEc ← | conserved protein of unknown function (C‑terminal end) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 238,265 | 0 | G | T | 21.1% | 65.6 / 3.1 | 35 | pseudogene (184/285 nt) | ybfEc | conserved protein of unknown function (C‑terminal end) |
| Reads supporting (aligned to +/- strand): ref base G (6/21); new base T (6/2); total (12/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTCACAG > BS168_reference_genome/238129‑238402 | atgaGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGTTCATACCGACTATGCGGGCGTGTTCGGATTTTTGTTTGAAGCGGTCAGAAATTTGa > 7:9401/1‑138 (MQ=255)atgaGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTAGGTTTTTTTTTTTAAACGTTGAGAAATTTTaa > 7:124191/1‑139 (MQ=255)atgaGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGAGCGCGTGTGGAGGATTTTGTTTTTAGAGTTTAGAAATTTg > 7:124187/1‑137 (MQ=255) aGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTGGGTTTTTTGTTTTAAGCGGTCAGGAAATTTAACTTttgttg > 1:56480/1‑138 (MQ=255) aaGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTGGGGGTTTTTTTTGTAGCGTTGTGGAATTTTAAGTTTTGGTGGAAATg > 3:192765/1‑139 (MQ=255) aTCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGa < 5:97357/139‑1 (MQ=255) tGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGaa < 5:275351/135‑1 (MQ=255) tCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGGTGGGGTTTTTGTTTGTAGCGGTGAGAAATTTGAACTTTTGTGGTAAATGGGGGTACAATaaa > 8:155347/1‑138 (MQ=255) cTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTGGGTTTTTTGTTTTAATCGTTCAGAAATTTGAACTTTTATTTTAAAAGGGGAAAAAATAAAAACCCAAAAAATGCCGAta > 7:140067/1‑137 (MQ=255) tttGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTGGGAGTTTTGTTTGAAGCGTTCAGAAATTTTAACTTTTCTTGAAAATGGGGGAACAATAAAAACCCAAAAAAGGCCGATCAAGGc > 7:154421/1‑138 (MQ=255) tATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCaaaa < 1:144671/138‑1 (MQ=255) tATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCaaaa < 3:120487/138‑1 (MQ=255) tCTAATACGAATTCGATCATACCTACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTTAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGt < 1:136725/138‑1 (MQ=255) aTACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAAt < 4:28793/138‑1 (MQ=255) aCTATGCGGGCGTGTTCGGATTTTTTTTTGAAGCGTTGAGAAATTTTAACTTTTGTTGTAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACAATTGTACAGCTAATTATattaattaatta > 7:128211/1‑138 (MQ=255) cGGGCGTGTTGGGGGTTTTTTTTGAAGCGGTCAGAAAATTGAACTTTTCTTGAAAATTGGGAAACAAAAAAAACCCAAAAAATGCCGCTCAAGGCAAAAAACAATTGTTCAGCGAATTATATTTATTAATTAAAACGAg > 8:207918/1‑139 (MQ=255) ggCGTTTTGGGATTTTTGTTTGAAGCGTTAAGAAATTTTAAATTATATTGAAAAAGGGGAAACAAAAAAAACCCAAAAAAGGCCGCACAAGGCAAAAAACAATTTTACATCTAATTCTATTAATTCATTAAAAAGAATg > 8:239488/1‑139 (MQ=255) tttttGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAg < 5:29446/139‑1 (MQ=255) ttttGTTTGAAGCGTTCATAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAg < 1:127422/138‑1 (MQ=255) ttgtttgAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGaa < 2:170260/138‑1 (MQ=255) tttgAAGCGTTCAGAAATTTGAACTTTTCATGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGta < 4:24455/138‑1 (MQ=255) tttgAAGCCTTCAGAAATTTGAAATTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGtat < 5:197687/139‑1 (MQ=255) gAAGCGTTCATAAATTTGAACTTTTCTTGAAATTGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATaa < 7:129083/138‑1 (MQ=255) aaGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACg < 7:239488/139‑1 (MQ=255) gTTCAGAAATTTTAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGttat < 7:6882/139‑1 (MQ=255) ttCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAgt < 8:278380/55‑2 (MQ=255) ttCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAgg > 7:278380/1‑55 (MQ=255) cAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGttatta < 4:275454/138‑1 (MQ=255) aGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTaa < 4:251349/138‑1 (MQ=255) aTTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTca < 3:137316/139‑1 (MQ=255) aTTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTca < 8:124191/139‑1 (MQ=255) aTTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTca < 8:128211/139‑1 (MQ=255) aTTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTca < 3:53415/139‑1 (MQ=255) aTTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTca < 8:9401/139‑1 (MQ=255) aTTTGAACTTTTCATGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTca < 8:124187/139‑1 (MQ=255) tGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTCACAg > 1:248594/1‑139 (MQ=255) | ATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAGAAGTATAACGTTATTAATGTCACAG > BS168_reference_genome/238129‑238402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |