BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	137	A→C	6.0%	intergenic (–/‑2841)	– / → eryR	–//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	137	0	A	C	6.0%	17574.4 / 216.6	7019	intergenic (–/‑2841)	–/eryR	–//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (3336/3106); new base C (35/534); total (3378/3641)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7268 total aligned reads displayed.

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  Exported/1‑275

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGaaaaaatc                                                                                                                                           >  3:111586/1‑137 (MQ=255)

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGATCGGAAATGAAAGAA‑Gc                                                                                                                                          >  6:244603/1‑139 (MQ=255)

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑Gc                                                                                                                                          >  7:226247/1‑139 (MQ=255)

  catcatCCAAAGTTGGATAGTTTTTTTTATGTCGCATATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATATAAACATAGCGTTAATATTAGCAGTAACTTAATAGACCGCAATTGAAAAAA‑GCCa                                                                                                                                        <  3:302511/139‑1 (MQ=255)

  cATCCTCCAATGTTGGAGATTGTGTTTTATGTCGCAAATTTTAATGTTTCTGGTTAACCTTATCAAATTTTCGTTTATTTAATACAAACATCGCGTTAAAATTAGCAGTAACTTAATACAACGTAAATTAAAAAC‑GCCa                                                                                                                                        <  6:221679/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGTGTGTGATTTTTATTTCGAAAATATTATTGTTTCTGGTGAACCTTATAAAATTTTCTTTTATTTAATAGAAACATAGCGTTAAAATTAGCAGTAAATTAATAGAACGTAAATTAAAAAA‑GCCa                                                                                                                                        <  2:93736/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCa                                                                                                                                        <  3:284025/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCa                                                                                                                                        <  7:168417/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATACAAACATCGCGTTAAAATTAGCAGTAACTAAATACACCGGAAATTAAAAAA‑GCCa                                                                                                                                        <  4:158375/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGACGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGAGGTAAAATTAGCATTAAATTAATATACCTGAAATTAAAAAA‑GCCa                                                                                                                                        <  7:66012/139‑1 (MQ=255)

  cATCCGCCAAAGTTGTAGAGTGAGTTTTATGCCGCAAATATAAATGTTTCTGGTCAACCTTATCAAATTTTCTTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATATAACGCAAATGAAAAAC‑GCCa                                                                                                                                        <  4:228262/139‑1 (MQ=255)

  aagcctccAATGTTGTAGTGTTTTTTTTATGTCCCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTGTTTTATTTAATATAAAAATAGCGGTAAATTTAGCATTAAATTAATAGAACGCAATTTAAAAAA‑GCCa                                                                                                                                        <  4:207610/136‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCa                                                                                                                                        <  6:68336/137‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCAct                                                                                                                                      >  3:53594/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCAct                                                                                                                                      >  8:146413/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCAct                                                                                                                                      >  6:272603/1‑139 (MQ=255)

     cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCActc                                                                                                                                     >  5:174312/1‑139 (MQ=255)

     cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCActc                                                                                                                                     >  3:154679/1‑139 (MQ=255)

     cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCActc                                                                                                                                     >  8:102224/1‑139 (MQ=255)

        ccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCa                                                                                                                                  <  7:84649/139‑1 (MQ=255)

         cAATGTTGGAGAGTGTTTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATAGAAAAATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCat                                                                                                                                 <  4:186121/139‑1 (MQ=255)

         cAAAGTTGTAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCGGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATCGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAA‑GCCACTCTCat                                                                                                                                 <  8:54827/139‑1 (MQ=255)

          ataGTTGTAGAGTTTTTTTTTTGTCGAAAATATTAATTTTTCTGGTTAACATTATCAATTTTTCGTTTATTTATTAGAAAAATAGGGGTAATATTTGCTGTAATTTATTTGAACGGAATTTAAAAAA‑GCCACTCTCata                                                                                                                                <  2:216307/137‑1 (MQ=255)

          aaaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCata                                                                                                                                <  8:280552/139‑1 (MQ=255)

           aaGTTGGAGATTTAGTTTTATGTCGCAAATATTAATTTTTCTGGTGAACCTTATCAAATTTTGGTTTATTTAATAGAAACAAAGCGGTAAAATTAGCAGTAAATTAAAAGAACGGAAATTAAAAAACGCCACTCTCata                                                                                                                                <  2:25991/139‑1 (MQ=255)

            aGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATg                                                                                                                              <  3:13103/139‑1 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTAtt                                                                                                                         >  5:282106/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTAtt                                                                                                                         >  7:298803/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTAtt                                                                                                                         >  7:261277/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTAtt                                                                                                                         >  5:153952/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTAtt                                                                                                                         >  8:23531/1‑139 (MQ=255)

                   tattgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTAAATATAAACATAGCGGTAAAATTTGCTGTAACTTATAAGAACGTAAATTAAAAAA‑GCCACTCTCATATGCTATTg                                                                                                                        <  5:118552/135‑1 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTa                                                                                                                    >  7:20904/1‑139 (MQ=255)

                      tgaggTTTATGGCGCAAATATTAATGTTTCTTGTGAACCTTTTCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATGAGAAGTAACTTAAGAGAACGGAAATGAAAAAA‑GCCACTCTCATATGATATTGGCTa                                                                                                                    >  4:116640/1‑139 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  1:265772/1‑138 (MQ=255)

                        agtTTTATGACGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATACAAACATAGCGGTAAAATTAGCAGTAAATTAATAGACCGCAAATCAAAAAA‑GCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  4:137060/139‑1 (MQ=255)

                              aTGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATAAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACGAACCtt                                                                                                            >  1:86697/1‑139 (MQ=255)

                               tGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                                            >  4:298833/1‑138 (MQ=255)

                                gTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                                            >  5:241292/1‑137 (MQ=255)

                                 tCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAA‑GCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  6:121708/130‑1 (MQ=255)

                                  cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCTGTAAAATTAGCAGTAACTTAATAGAAAGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTatc                                                                                                        >  6:92127/1‑137 (MQ=255)

                                  cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAATTGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  6:102826/1‑139 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCat                                                                                                                                 >  4:75630/1‑112 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  6:146579/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  7:132671/1‑137 (MQ=255)

                                       taatTAATGTTTCTGGTTACCCTTATCAAATTTTCGTTGATTTAATTGAAACATAGCGGAAAATTTAGCATTAACTTAATACACCGGAAATCAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  6:292654/137‑1 (MQ=255)

                                       atatTAATGTTTCTGTTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGACCGTAAATTAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  2:234578/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTTAACATTTTAAATTTTTCGTTTATTTAATAGAAAAATAGCGTTAAAATTAGCATTAACTTAATATACCGCAAATTAAAAAC‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  2:108829/139‑1 (MQ=255)

                                            aaTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAAAAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  3:262840/139‑1 (MQ=255)

                                             aTGTTTATGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACTTAGCGGTAAAATTAGCAGTAACTTAATACAACGTAAATTAAAAAC‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  8:74663/138‑1 (MQ=255)

                                             aTGTTTATGGTGAACATTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGTAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  2:65639/138‑1 (MQ=255)

                                                tttCTGGTGAACCTTATCAAATTTTGGTGTATTTAAAACAAAAAAAGCGGAAAAATAAGCAGAAAATTAATAGAACGCAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  8:259444/138‑1 (MQ=255)

                                                tttCTGGTCAAACTTAAAATATTTTCTTTTATTAAATAGAAAAATAGCGGAAAAATAACCATAAAAATAATACAACGTAAATAAAAAAC‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  2:139511/138‑1 (MQ=255)

                                                tttATGGTGAAACTTATCAAATTTTAGTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAATTGAAAAAA‑GACACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  8:186338/138‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         <  1:16297/138‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAA‑GCCACTCTCATATGCTATTGTCTACCAACATTTAGCGAGAATGAATTAATc                                                                                         >  8:300145/1‑138 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  1:185818/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  3:92676/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  3:222332/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  3:243848/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACAAACCTTTAGAGAGAATGACTTAATcc                                                                                        >  1:142303/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         >  1:42507/1‑138 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGCAAATGAAAAAA‑GCCa                                                                                                                                        <  4:252028/91‑1 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  1:100127/1‑139 (MQ=255)

                                                     ggTTAAACTTATAAAATTTTGGTTTATTAAATAGAAACATCCGGGTAAAATTCGCATTAACTTAATTGACCGCAAATTAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  8:124107/138‑1 (MQ=255)

                                                      gcgAACCTTATAAATTTTTCGTTTATTAAAAACAAAAATAGCGGAAAAATTACCAGAAAATAAATAGAACGTAAATAAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  5:26358/137‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  7:239209/139‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  2:41616/139‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  5:299689/139‑1 (MQ=255)

                                                         aaCCTTATCAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAATACGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  5:219949/139‑1 (MQ=255)

                                                          aCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  3:74705/138‑1 (MQ=255)

                                                          aCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  3:198801/138‑1 (MQ=255)

                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  5:65158/129‑1 (MQ=255)

                                                                       ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  8:211027/138‑1 (MQ=255)

                                                                               tttAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTtat                                                            >  2:194507/1‑138 (MQ=255)

                                                                                        aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  5:136993/95‑1 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  2:285120/1‑138 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  5:201037/1‑138 (MQ=255)

                                                                                           ttAGAGGTAAAATTAGATTTTTCTTAATATAACTTAAATGAAATAA‑GCCACTCTCATATGCTATTGGCTAACTACCTTTAGCGTGAATGACTTAATCCTGTACAGCCATACATGACTTCGACTTATTAGAGGCGCGAAc                                               <  6:190466/138‑1 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  1:291961/1‑139 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  2:170189/1‑139 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  7:46488/1‑139 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  4:2759/1‑139 (MQ=255)

                                                                                                   aaaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                        <  5:82917/138‑1 (MQ=255)

                                                                                                   aaaaTTAGCAGTAACTTAAAAGAACGGAAATGAAAAAC‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                        <  5:49104/138‑1 (MQ=255)

                                                                                                    aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATaa                                      >  1:22596/1‑139 (MQ=255)

                                                                                                         aGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCACCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                                 >  1:116790/1‑139 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc                            <  3:178535/137‑1 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                          >  4:50352/1‑139 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                          >  6:167026/1‑139 (MQ=255)

                                                                                                                      aaaaaCGTAAACAAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc                    <  1:245127/136‑1 (MQ=255)

                                                                                                                          aCGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgcg                 >  7:150167/1‑138 (MQ=255)

                                                                                                                           cGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 >  7:110194/1‑73 (MQ=255)

                                                                                                                           cGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  8:164411/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  7:279056/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  1:64043/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  7:186670/1‑139 (MQ=255)

                                                                                                                              aaaTCAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACaa            <  1:204127/139‑1 (MQ=255)

                                                                                                                                    aaaaa‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  6:44641/139‑1 (MQ=255)

                                                                                                                                        a‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCttat     >  4:99040/1‑136 (MQ=255)

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAA‑GCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  Exported/1‑275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`