BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,729	T→G	6.2%	intergenic (+14/‑142)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,729	0	T	G	6.2%	14582.6 / 184.2	4866	intergenic (+14/‑142)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (386/4067); new base G (392/9); total (780/4086)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5156 total aligned reads displayed.

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3591‑3872

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑t‑tt                                                                                                                                               >  3:44739/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑t‑tt                                                                                                                                               >  4:276549/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑t‑tt                                                                                                                                               >  5:136123/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑g‑tt                                                                                                                                               >  5:238484/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑g‑tt                                                                                                                                               >  5:171100/1‑139 (MQ=255)

     aCTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑g‑ttttt                                                                                                                                            >  5:277035/1‑139 (MQ=255)

       tAAAAATCAGTTTCATCAAGCAATGAAACAAGACAAAGTAAACAATTTAAGTAACATTACTTATGAGCAAGTATTGTATATTTTTAATAGTTATATATTAATTAACGGGAGGAAATAATTCTATGAGTAGC‑A‑TATTTat                                                                                                                                          >  8:202396/1‑138 (MQ=255)

             tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTgg                                                                                                                                    <  8:24273/139‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGa                                                                                                                                   <  6:131184/138‑1 (MQ=255)

                     atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTa                                                                                                                             <  4:18194/138‑1 (MQ=255)

                     atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTa                                                                                                                             <  4:47163/138‑1 (MQ=255)

                      tcaAGCAATTAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGG‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  2:271415/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  3:178525/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  4:160711/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  5:218376/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  1:253331/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  1:234282/139‑1 (MQ=255)

                      tcaACCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  6:242591/139‑1 (MQ=255)

                      taaaccAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  1:142424/134‑1 (MQ=255)

                      taaaGCAATGAAACACGCCAAAGTAAAAAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTaca                                                                                                                           <  6:290295/137‑1 (MQ=255)

                        aggCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGAAAAGTTACACg                                                                                                                         <  1:213/137‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTTATAGTTATCTTTTATTTATCGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  3:203738/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  3:279098/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  3:22362/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  1:119565/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                    <  7:251330/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                    <  7:170334/139‑1 (MQ=255)

                               gAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTAATTTGGTAATTTAAAAGtttaaa                                                                                                                   >  8:152620/1‑134 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                 <  6:271371/139‑1 (MQ=255)

                                 aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                 <  8:262067/138‑1 (MQ=255)

                                    acGCAAACGTAAAAAATTTAAGTACCATTACTTATTAGAAAGTATTGTCTATTTTTAATAGTTATCTATTATTTACCGGGAGTAAATAATTCTATTAGCCGC‑‑‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  1:94960/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  5:83551/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  4:135140/139‑1 (MQ=255)

                                      gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTTATTTTGTAATTTATACGTTTATAAATTGAAt                                                                                                           >  4:231874/1‑139 (MQ=255)

                                       ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                          <  4:256197/139‑1 (MQ=255)

                                          aaGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTAAATTTGTAAA‑TTAAACCGTAATAAAAGGAAttgtg                                                                                                       >  2:219873/1‑135 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  1:277808/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  6:92914/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACAATTACTTATGAGCAAGTCTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  4:69832/139‑1 (MQ=255)

                                              aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  2:245490/138‑1 (MQ=255)

                                                aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAAtttt                                                                                                                                     >  2:199066/1‑102 (MQ=255)

                                                   atatAAGTACCATTACTTATGAGCAAGTAATGTCTATTTTTAATAGTTATCTATTAATTAACGGGACGAAATAATTCTATGAGGCGC‑T‑TTTTTAAATTTGGAAACTTACACGTTACTAAAGGGAATGGAGATAAattat                                                                                              <  6:145846/136‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  6:41084/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  1:3211/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:72479/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:289549/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  5:29692/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:268140/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  1:165669/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  8:73078/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTAATTTTTTAATTTAAACGTTTCTAAATGGTATGGTGAATAattatt                                                                                             >  8:132862/1‑139 (MQ=255)

                                                    tatAAGTACCATTACTTATGAGCAAGGATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAACTAATTCTATGAGTCGC‑T‑TTTTTAACTTTGGAAAGTTAACCGTTACTAAAGGGACTGGAGATAAattatt                                                                                             <  2:120449/137‑1 (MQ=255)

                                                       aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  8:222196/138‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  7:191027/139‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  4:204108/139‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  8:177890/139‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  7:88276/139‑1 (MQ=255)

                                                         gTACAATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  7:210083/139‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  7:232700/138‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTTATTTTTTAATTTAAAAGTTTATAAATTGAATTGAGATAAATTATtatatataa                                                                                     >  1:55480/1‑138 (MQ=255)

                                                              aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGT‑TTTTTAATTTTTTAATTTTAAAC‑ATACGAAAAGGAATTGGTATAAATTATtatatataaa                                                                                    >  1:187352/1‑136 (MQ=255)

                                                                  cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                               <  2:180026/139‑1 (MQ=255)

                                                                    tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTAATTTTTAAAATTAAAAGTTTATAAAAGGAATGGAGATAAATTATTATATATAATAATGACa                                                                             >  4:302516/1‑139 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTTATTTTTATATTTTCACCATAAAAAATGGAAATGTGATAAATTATTTTATATTATACAGACAGCt                                                                          >  3:102540/1‑139 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTATATTTTGTTAGTTAAACGTTAAGAAACGGAATTGTGAATAATTATTATATATAATAATGAAAGcg                                                                          >  6:217052/1‑138 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTTATTTTGTTAATTATACCTTTATAAAAGGAATGGAGATTAAATATTTTATATAAAAAAGACAGCt                                                                          >  1:75600/1‑139 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTAATTTTGAAATTTAAAAGTTTATAAATTGAATTGATATTAAATTTTATATATAATAATTACAGCt                                                                          >  8:56384/1‑139 (MQ=255)

                                                                          cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTTATTTTTTTATTTTCAAGTTAATTAAAGTAATGGAGTTAAATAATTATATATAATAATGAAAGCtta                                                                        >  6:70830/1‑137 (MQ=255)

                                                                          cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTTAATTGGAAAGTTAAAAGGTACGTAAGGGAAAGGAGATAAAATATTAATAATACTAAAGACAGCtta                                                                        >  8:30807/1‑137 (MQ=255)

                                                                           aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAATTAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  2:54062/139‑1 (MQ=255)

                                                                           aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  3:261725/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  5:67427/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  6:190807/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  5:190151/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGCAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  3:119100/139‑1 (MQ=255)

                                                                              tcttGTCTATTTTTAATAGTTCTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  2:30200/137‑1 (MQ=255)

                                                                               aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  5:47317/138‑1 (MQ=255)

                                                                                ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                 <  7:41104/139‑1 (MQ=255)

                                                                                       tttttAATATTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  5:151652/137‑1 (MQ=255)

                                                                                       tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  7:56384/137‑1 (MQ=255)

                                                                                       tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                           <  3:78884/138‑1 (MQ=255)

                                                                                       tttttAATAGTTATCTATTA‑TTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  3:62530/139‑1 (MQ=255)

                                                                                        ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTAAATTTTTAAAGTTACACGTTTCAATAGGGTATGGTGATAAATTATTAGATATACTACTGAAAGCTTCAAAGAAGCTAAagag                                                         >  7:149474/1‑139 (MQ=255)

                                                                                              atgTTATCTATTATTAAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTAACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTGCCAAGAAGCTAAAGAGGTCCCt                                                   <  1:100148/137‑1 (MQ=255)

                                                                                                gTTATCTATTATTTAACGGGATTAAATAATTCTATGAGTAGC‑T‑TTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTa                                                  <  4:94806/138‑1 (MQ=255)

                                                                                                         tattTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTAATTTTGTAATTTTAACGTTACTAAATGGAATGGAGATAAATTATTTTATTTAATAATTACACCTTACAAAAATCTAAATAGGTCCCTAGCGCCTACgg                                        >  8:279428/1‑139 (MQ=255)

                                                                                                                   gaggaAATAATTCTATGAGTCGC‑G‑TTTTTTAATTTTGTAAGTTTAACAGTTCAAAAAGGAATGGAGATAAAATATTATATATAATAAATAAAAGATACAACAAGAGAAATAGGTCCCTATCGGCAACGGGGAAttttgg                              >  6:110425/1‑136 (MQ=255)

                                                                                                                     ggaAATAATTCTATGAGTCGC‑G‑TTTTTTAATTTTGTATGTTTCCCGGTGCTAAAGGGAATTGGGATTAATTTTTTTTTATTCGAATGACAGCGTGCAAGAAGCGAAATAGGTGGCTAGCGCCGACGGGGGTTTTggggg                            >  2:15414/1‑135 (MQ=255)

                                                                                                                          taatTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                       <  4:106945‑M1/139‑10 (MQ=255)

                                                                                                                               cTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                   <  8:108652‑M1/138‑14 (MQ=255)

                                                                                                                               cTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                   <  2:7975‑M1/138‑14 (MQ=255)

                                                                                                                               cTATGAGTCGC‑T‑TTTTTAAATTAGGAAAGTTACACGTTACTAAAGGGAATCGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                   <  3:301677‑M1/138‑14 (MQ=255)

                                                                                                                                  tGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTATAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCATCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  3:240797‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  6:166147‑M1/139‑18 (MQ=255)

                                                                                                                                   gAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACTCTTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:115850‑M1/138‑18 (MQ=255)

                                                                                                                                   gAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:136826‑M1/138‑18 (MQ=255)

                                                                                                                                    aGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  3:139928‑M1/139‑20 (MQ=255)

                                                                                                                                    aGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga              <  5:117593‑M1/138‑19 (MQ=255)

                                                                                                                                     gTCGT‑T‑TTTTTAAATTTGTAATGTTAGACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  3:156815‑M1/138‑20 (MQ=255)

                                                                                                                                     gTCGA‑T‑TTTTTAAATTTGGAAATTTACACGTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  5:260125‑M1/138‑20 (MQ=255)

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3591‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`