BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,735	A→T	9.0%	intergenic (+20/‑136)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,735	0	A	T	9.0%	14896.8 / inf	5111	intergenic (+20/‑136)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (271/4324); new base T (497/18); total (769/4342)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5214 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3597‑3877

   aaaaTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTT‑AAt                                                                                                                                               >  1:202691/1‑139 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTT‑AAtttt                                                                                                                                            >  3:69542/1‑138 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTg                                                                                                                                            >  8:177785/1‑139 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTg                                                                                                                                            >  6:271154/1‑139 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGa                                                                                                                                          <  4:41667/138‑1 (MQ=255)

           tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTT‑AATTTTGAAAg                                                                                                                                       >  6:70167/1‑139 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTCTGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTa                                                                                                                                   <  5:230297/138‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAAATTAAGTACCATTTCTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTa                                                                                                                                   <  4:147209/138‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCTAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  4:194646/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  3:53180/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  7:86763/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  3:146599/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  7:229887/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  7:115085/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  4:269387/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCAATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  5:41803/139‑1 (MQ=255)

                tcaAGCAATCAAACACGCCAAAGTAAACAATATAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  3:11001/139‑1 (MQ=255)

                cacaGCAATGAAACACGCCAAAGTAATCAATTTAAGTACCATTACTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                 <  3:164666/136‑1 (MQ=255)

                  aataaaTGAACCACGCAAAAGTAAACAATTTAAGTACAATTACTTATTAGTTAGTATTGTCTATTTTTAATTGTTATCTATTATTTAACGGGAGGAAATATTTCTATGATTCGCTTTTTTA‑AATTTTGAAAG‑TTacaag                                                                                                                               <  6:213593/135‑3 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTTTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACg                                                                                                                               <  2:4468/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACg                                                                                                                               <  8:243471/139‑1 (MQ=255)

                     cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGGTTTT‑TATTTTGAAAT‑TTacaacct                                                                                                                             >  5:99270/1‑134 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACt                                                                                                                          <  4:217773/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACt                                                                                                                          <  7:170334/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAAATTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATTAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACt                                                                                                                          <  1:120211/139‑1 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTaaa                                                                                                                       <  1:141030/139‑1 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTaaa                                                                                                                       <  7:39670/139‑1 (MQ=255)

                           aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTaaa                                                                                                                       <  1:227262/138‑1 (MQ=255)

                           aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTaaa                                                                                                                       <  4:24272/138‑1 (MQ=255)

                              acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGa                                                                                                                   <  6:2368/139‑1 (MQ=255)

                                  cAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGaaaat                                                                                                                                      >  6:289781/1‑115 (MQ=255)

                                     aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGgaga                                                                                                            <  1:47043/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATa                                                                                                          <  3:29046/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATa                                                                                                          <  2:168910/139‑1 (MQ=255)

                                        aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATa                                                                                                          <  5:164198/138‑1 (MQ=255)

                                            aaTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAa‑tta                                                                                                    <  2:138952/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAa‑ttatt                                                                                                  <  7:268140/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAa‑ttatt                                                                                                  <  4:87632/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTATTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAa‑ttatt                                                                                                  <  5:62364/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTGTTTTT‑AATTTTTAATGTTTAAACTTTA‑TTAAGGGAATTTGGTTAAa‑ttatt                                                                                                  >  3:268234/1‑139 (MQ=255)

                                                tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑ttatta                                                                                                 <  7:208424/138‑1 (MQ=255)

                                                tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTGTTTTATAATTTTG‑AAG‑TTAAAAATTACAAAAAGGTATGGAGATAAA‑ttattat                                                                                                >  8:33401/1‑138 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAg                                                                                                <  2:289648/138‑1 (MQ=255)

                                                   gTACCCATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGata                                                                                             <  2:9301/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGata                                                                                             <  3:229857/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTAAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTT‑TATTTTTTAAT‑TTAAAAGGTTATAAAAAGAAATGATATAAA‑TTATtatata                                                                                             >  8:107393/1‑139 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTT‑AATTTTGTAAT‑TTAAAAGTTACTAAAATGTATGGTGATAAA‑ATATtatatata                                                                                           >  8:152458/1‑138 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGAtata                                                                                           <  8:202337/138‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATAtac                                                                                          <  7:13548/139‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATAtac                                                                                          <  1:161251/139‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGa                                                                                    <  7:70691/139‑1 (MQ=255)

                                                                    cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTT‑AATTTTGTAAT‑TTTAACGTTAATAAATGGAATTGATATTAA‑TTTTTTTATATAATAATGACaaatta                                                                             >  2:70702/1‑133 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGTAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCa                                                                           <  2:194045/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTAGTGACAGCTTCCa                                                                           <  6:141916/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCa                                                                           <  6:92737/139‑1 (MQ=255)

                                                                     aaGTATTGTCTAATTTTAATAGTTTTCTATTTATTAACGGGTGGTAATTATTCTATGATTCGGTTTTTTA‑AATTTGGTAAT‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:184818/139‑1 (MQ=255)

                                                                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTT‑TATTTTGAAAGATTAACCTTTAAT‑AATGGAATTGAGATAAATTTATTA‑AAATAATAATGAAAGCCTCCa                                                                           >  6:125802/1‑138 (MQ=255)

                                                                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  1:182652/139‑1 (MQ=255)

                                                                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  1:269281/138‑1 (MQ=255)

                                                                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                 <  3:38470/139‑1 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAaga                                                               <  3:211447/139‑1 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAaga                                                               <  1:70321/139‑1 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                <  4:292282/138‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTT‑AATTTTGTAAT‑TTAAACGTTTATAAATGGAATGGAGATAAA‑TTATTTGATATAATAATGACAGCTTCAAATAAGCTAAatag                                                              >  3:251748/1‑139 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGTAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTTGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTAACAGCTTCCAAGAAGCTAAagag                                                              <  6:188114/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACAAGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  7:190779/138‑1 (MQ=255)

                                                                                       aTAGTTATATATTATTTAACGGGAGGAAATAATTCTATTAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGTGAATGTAGATAAA‑TTATTAGATATACTACTGACAGATTCCAAGAAGCTAAAGAGGTccc                                                         <  7:250235/139‑1 (MQ=255)

                                                                                        atgTTATCTATTATTAAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TAACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTGCCAAGAAGCTAAAGAGGTCCCt                                                        <  1:100148/137‑1 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTT‑AATTTGGTAAT‑TTACACGGTAATAAAGGGAATGGGGATAAA‑ATATTAGATATACTACTGACAGCTTCCATAAAGCTAAAGAGGTCCCTAgc                                                     >  3:84816/1‑139 (MQ=255)

                                                                                            tATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTT‑AATTTTGGAAG‑TTTCACGTTACTTAATGGAATGGAGATTAA‑TTTTTTTATATACTAATGAAAGCTTGCAAGCAGCTAAAGAGGTCACTAgcg                                                    >  5:111097/1‑139 (MQ=255)

                                                                                                    attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                             <  4:62074/138‑1 (MQ=255)

                                                                                                     tttAACGGGAGGAAATTATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                            <  7:150297/138‑1 (MQ=255)

                                                                                                             gatgaattAATTCTATGAGTCGCTTTTTTA‑AATTTGTAAAG‑TTACACGTTAATAAAGGGAATGGAGATAAA‑TTATTAGATATTCTACTGACAGATTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                   <  2:211700‑M1/136‑3 (MQ=255)

                                                                                                              aggaAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                  <  5:147981‑M1/139‑4 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  1:2470‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGCTTTTTTA‑AATTTCGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  2:259227‑M1/139‑8 (MQ=255)

                                                                                                                      atTCTATGAGTCGCTTTTTTA‑AATTTGGTAAG‑TTACACGTTACTAAAGGGAATCGAGATAAA‑TTATTAGATATACAACTGACAGCATCTAAGACGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                          <  6:160559‑M1/139‑12 (MQ=255)

                                                                                                                      atTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                          <  8:57447‑M1/139‑12 (MQ=255)

                                                                                                                            ttagtCGATTTTTTA‑AATTTTTAAAG‑TTACACGTTACAAAAGGGAATGGAGATAAA‑TTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:268688‑M1/137‑18 (MQ=255)

                                                                                                                            tGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGTAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:128403‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGTTATACTACTGACAGCTTACAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:289568‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTAATAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:251511‑M1/139‑18 (MQ=255)

                                                                                                                             ttgtagcttttTTA‑ACTTTGGAAAG‑TTACACGTTAATAAAGGGAATGGAGATAAA‑TTATTAGATATAATACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  4:170816‑M1/133‑18 (MQ=255)

                                                                                                                             gAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTATAGGGAATGGAGATAAA‑TTATTAGATATACTACTCACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:40963‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGTAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  1:292907‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGTAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  8:263838‑M1/138‑18 (MQ=255)

                                                                                                                              aGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGTAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  3:191403‑M1/139‑20 (MQ=255)

                                                                                                                              aGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  8:223026‑M1/139‑20 (MQ=255)

                                                                                                                               gTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                 <  4:115736‑M1/139‑21 (MQ=255)

                                                                                                                                 cGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag               <  8:282364‑M1/139‑23 (MQ=255)

                                                                                                                                    tatttta‑aATTTGGAAAC‑TTACACGTTACTAAAGGGAATGGAAATAAA‑TTATTATATATACTACTGACAGCTTCCAATAAGGTAAAGAGGTCCCTAGCTCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  2:109384‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAG‑TTACACGTTACTAACGGGAATGTAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  8:73604‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  7:55731‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:77948‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:209322‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TCATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:29124‑M1/137‑26 (MQ=255)

                                                                                                                                       tttA‑AATTTTGAAAG‑TTACACGTTACTAAAGGTAATGTAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGGCCCTATCGCCTACGGGGAATTTGgggtacattgaataaggaagagtatga          <  8:129867‑M1/138‑28 (MQ=255)

                                                                                                                                       tttA‑AATTTGGAAAG‑ATACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga          <  2:232585‑M1/138‑28 (MQ=255)

                                                                                                                                          a‑aaTTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  5:132227‑M1/138‑31 (MQ=255)

                                                                                                                                          a‑aaTTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  1:85408‑M1/138‑31 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAA‑TTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3597‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`