BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,741	G→T	9.0%	intergenic (+26/‑130)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,741	0	G	T	9.0%	15153.2 / inf	5080	intergenic (+26/‑130)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (157/4332); new base T (493/68); total (672/4408)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5226 total aligned reads displayed.

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3603‑3877

aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTg                                                                                                                                          >  3:226510/1‑139 (MQ=255)

 tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTgg                                                                                                                                         <  1:62814/139‑1 (MQ=255)

  cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCAATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                        <  2:185416/139‑1 (MQ=255)

  cAGTTTCATCAAGCAATGAAACACGCCAAAGAAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                        <  6:62935/139‑1 (MQ=255)

   aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                        <  3:33129/138‑1 (MQ=255)

   aGTTTCATAAAGCAATTAAACACTCAAAATTAAACAATTTAAGTACCATTAGTTATGAGAAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGTAAATAATTCTATGAGTCGCTTTTTTAATTTTggt                                                                                                                                        <  6:154122/138‑2 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTa                                                                                                                                  <  7:148775/138‑1 (MQ=255)

          tgaatcAATGAAACTCGCCAAAGTAAAGAATTTAAGTACCATTTGTTATGACGAAGTATTGTCTATCTTTTATAGTTATCTATTAATTCTCGGGAGTTAATTATTCTATGAGTCGATTTTTTAAATTTGGAAAGTTact                                                                                                                                <  4:109762/134‑2 (MQ=255)

          tcaatcaaTTAAACACGCCAAAGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTTTCTATTTTTAATAGTTATCTATTTTTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  3:79265/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  6:138061/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  2:227139/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  3:182620/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  5:249742/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  3:159758/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  8:222824/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCAATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  3:247656/139‑1 (MQ=255)

          tcaAGCAATGAAACAAGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  2:161208/139‑1 (MQ=255)

            aacccaTGAAACACGGCAAACTAAACAAATTAACTAACAATAAATATTTCGAAATATTGTATATTTTTTATTCTTAAGTATTATTTAAAGGGAAGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                              <  7:35072/134‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                              <  5:74922/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                              <  3:281199/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                              <  7:248807/139‑1 (MQ=255)

            aaGCAATGAAACAAGCCAAAGTAAACTATTTAAGTACCATTACTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                              <  6:229420/139‑1 (MQ=255)

             aGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTTTTTTGTGAAGTTAAACgg                                                                                                                             >  6:182564/1‑138 (MQ=255)

                    aaaCACGACAAAGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                      <  4:39321/139‑1 (MQ=255)

                        acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTTTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                  <  8:262191/139‑1 (MQ=255)

                        acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                  <  5:129549/139‑1 (MQ=255)

                           ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                               <  5:160329/139‑1 (MQ=255)

                                gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                          <  2:38335/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  4:190636/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  4:165898/139‑1 (MQ=255)

                                  taaCATTTTTAGTATAATTTCTTATGATGAAGTATTTTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTATATGAGTCGGTTTTTTAAATTTGGAAATTTAGACGTTACTAAAGGGAATGGAGATa                                                                                                         <  7:167256/137‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  5:56956/138‑1 (MQ=255)

                                   aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  3:106281/139‑1 (MQ=255)

                                      aaTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatta                                                                                                    <  7:113055/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  1:42253/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:203846/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:51715/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:145651/139‑1 (MQ=255)

                                        attAACTACCATTACTTATGAGGAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  1:198103/138‑1 (MQ=255)

                                           aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  4:84337/138‑1 (MQ=255)

                                           aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  7:67133/138‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  7:224997/139‑1 (MQ=255)

                                             cagccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  1:120172/136‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAATTTTCACGGTAGTATAGGGAATTGTGATTAATTTTTTGATAtac                                                                                          >  8:12675/1‑139 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                           <  1:263720/138‑1 (MQ=255)

                                                  aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTTTTAGTTAAAAGTTACATAAAGGAATGGAGTTAAATTATTTtatataat                                                                                         >  6:206631/1‑136 (MQ=255)

                                                       ttATGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAATTGTATGAGTCGCTTTTTTAAATTTGGAAAGATACCCGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  2:283054/139‑1 (MQ=255)

                                                         aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  3:45716/137‑1 (MQ=255)

                                                         aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  2:100000/137‑1 (MQ=255)

                                                         aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACa                                                                                  <  5:288869/138‑1 (MQ=255)

                                                           gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGTAAGTTTCAAGTTAATAAAAGGAATGGAGATAAATTATTATATATAATAAAGACAGca                                                                               >  8:173463/1‑138 (MQ=255)

                                                            aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGATATTTTCACGGTTATAAAAGGTATGTATGTAAATTATTATATTTAATAATGACAGCtt                                                                              >  1:242389/1‑139 (MQ=255)

                                                               atgTATTGGCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  7:8492/137‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGTGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTCAACGTAATCGAGATAAATTATTAGATATACTACTTACAGCTTCCa                                                                           <  8:281757/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:106592/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  8:3394/139‑1 (MQ=255)

                                                                aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGATAATTTCACGTTTCTAAATGGTATTGTGATTAATTTTTATATATAAAAAAGACAGCttgcc                                                                           >  7:133911/1‑135 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  8:200107/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  1:178342/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  3:123139/139‑1 (MQ=255)

                                                                    ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                      <  6:122241/139‑1 (MQ=255)

                                                                    ttGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                      <  6:3729/139‑1 (MQ=255)

                                                                       tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                   <  4:140294/139‑1 (MQ=255)

                                                                         tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                 <  1:221625/139‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGTAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGGTACTAAAGGGAATGGAGATAAATTATTAGTTATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  2:20509/138‑1 (MQ=255)

                                                                                 aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTAATATTGAATATTTAACGGTACTAAAAGGAATTTAGATATAATATTTTATATAATAATGACACCGTCACAGAAACTAAAGAGGtac                                                          >  4:240303/1‑136 (MQ=255)

                                                                                  tAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                        <  3:264331/139‑1 (MQ=255)

                                                                                  atgTTATCTATTATTAAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTAACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTGCCAAGAAGCTAAAGAGGTCCCt                                                        <  1:100148/137‑1 (MQ=255)

                                                                                                   aCGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                        <  6:87073/138‑1 (MQ=255)

                                                                                                       gaggaAATAATTCTATGTGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                   <  5:37913‑M1/139‑3 (MQ=255)

                                                                                                         ggaAAAAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggt                                 <  2:64450‑M1/139‑5 (MQ=255)

                                                                                                            aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  8:104157‑M1/139‑8 (MQ=255)

                                                                                                            aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  4:265530‑M1/139‑8 (MQ=255)

                                                                                                                atTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                          <  8:57447‑M1/139‑12 (MQ=255)

                                                                                                                   cTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                        <  6:284510‑M1/138‑14 (MQ=255)

                                                                                                                   cTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                        <  8:24316‑M1/138‑14 (MQ=255)

                                                                                                                   cTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                        <  1:1385‑M1/138‑14 (MQ=255)

                                                                                                                      tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:267031‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTAC‑GGGAATTTGgggtacattgaaaaagg                    <  3:63536‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:206095‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  8:149474‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTAGCTTTTTTAAATTTTGAAATTTACAAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:265713‑M1/138‑18 (MQ=255)

                                                                                                                        aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGCCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:285966‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:187678‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGCTTTTTTAAATTTGGAAAGTTACACGGTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTCGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  3:142429‑M1/139‑20 (MQ=255)

                                                                                                                         gTCGCTTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGGAATTGAGATAAATTATTAGATATACTAATGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  5:93846‑M1/138‑20 (MQ=255)

                                                                                                                         gTCGATTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  1:80682‑M1/138‑20 (MQ=255)

                                                                                                                               tttttAAATTTGGAAAGTTACACGTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  2:128860‑M1/138‑26 (MQ=255)

                                                                                                                               tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  4:287409‑M1/138‑26 (MQ=255)

                                                                                                                                ttttAAATTTGGAAAGTTACACGTTACTAACGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  8:73604‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:182669‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  2:87146‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  1:288375‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAATTTGGAAAGTTAAACGTTACTAAAGGTAATTGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  4:212784‑M1/137‑26 (MQ=255)

                                                                                                                                    aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  5:228438‑M1/138‑31 (MQ=255)

                                                                                                                                     tatTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCTAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  2:251711‑M1/138‑33 (MQ=255)

                                                                                                                                     attttggAAAGTTACAAGTTAATAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAACAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  1:158996‑M1/137‑33 (MQ=255)

                                                                                                                                     aaTTTGGAAAGTTACACGTTACTAAAGGGATTTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  3:206628‑M1/139‑33 (MQ=255)

                                                                                                                                     aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  4:58638‑M1/139‑33 (MQ=255)

                                                                                                                                     aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  6:262864‑M1/139‑33 (MQ=255)

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3603‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`