BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,625	A→C	6.9%	K252T (AAG→ACG)	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,625	0	A	C	6.9%	11859.9 / 148.8	4465	K252T (AAG→ACG)	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2497/1532); new base C (41/369); total (2546/1919)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4533 total aligned reads displayed.

AGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  Exported/4488‑4763

 gACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  6:109180/138‑1 (MQ=255)

 gACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  7:185051/138‑1 (MQ=255)

 gACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCGGGCTGGTTTATTGCTGATAATTCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  4:58161/138‑1 (MQ=255)

  aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  1:297713/1‑139 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  1:148023/1‑139 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  1:47992/1‑139 (MQ=255)

   atggattgaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCAGGCTCGCTGGTTTATTGCTGATAAATCTGGAGCAGTTGAGCTTGGGTCTCGCGGTATCATTGCAGCACTGGTGCCACATGGAAAGcc                                                                                                                                         >  8:129073/2‑138 (MQ=255)

     ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  4:175172/1‑139 (MQ=255)

     ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  1:186804/1‑139 (MQ=255)

      gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  3:104504/138‑1 (MQ=255)

      gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  5:144670/138‑1 (MQ=255)

        tggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCg                                                                                                                                   <  4:118263/139‑1 (MQ=255)

          gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTa                                                                                                                                 <  8:270087/139‑1 (MQ=255)

           aGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAt                                                                                                                                >  1:86527/1‑139 (MQ=255)

           aGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAt                                                                                                                                <  3:237949/139‑1 (MQ=255)

           aGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAt                                                                                                                                <  5:106689/139‑1 (MQ=255)

           aGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATAGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAt                                                                                                                                >  6:126278/1‑139 (MQ=255)

             gCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCAGGCTGGTTTAATGCTGAAAAATCTGGAGCCGGTTAGCTTGGTTCTCGCGGTATCATTGCAGCAATGGGCCCAGATGGAAAGCCCTCCCGTATCg                                                                                                                              >  1:208334/1‑139 (MQ=255)

             gCGGATAAAGTTGCAGGACCACTTCTGCGCGCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              <  1:126524/139‑1 (MQ=255)

              cGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  7:31181/130‑1 (MQ=255)

              cGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTAGCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              >  6:29589/1‑138 (MQ=255)

               gtatatAGTTGCATGAGGACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTTTTGCTTATATATCTGGAGCCGGTGAGGGTGGGTCTCGGGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            <  3:201362/137‑1 (MQ=255)

               ggATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            <  2:58185/139‑1 (MQ=255)

               ggATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            <  7:22148/139‑1 (MQ=255)

                gATAAAGTTGTAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  5:268995/1‑139 (MQ=255)

                gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           >  5:124730/1‑123 (MQ=255)

                gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  1:67412/1‑139 (MQ=255)

                gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  7:260118/1‑139 (MQ=255)

                gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  4:247265/1‑139 (MQ=255)

                  tAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGGTGGGTTGTTTATTGCTTATAAAGCGGGAGCCGGTGTGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCTGTTGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         <  2:204308/139‑1 (MQ=255)

                  tAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         <  7:106688/139‑1 (MQ=255)

                  tAAAGTGGCAGGACCACTGCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAAAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         <  2:18645/139‑1 (MQ=255)

                  tAAAGGTGCAGGACCCCTTCTGCGCTCGGCCCTTCCGGCTGGGTGGGTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         <  5:80124/139‑1 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGTCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCg                                                                                                                                   <  5:54360/121‑1 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCTCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGGTATCTacac                                                                                                                 >  8:147320/1‑139 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  2:33497/1‑139 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  3:148301/1‑139 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  1:166632/1‑139 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  3:254143/1‑139 (MQ=255)

                                  cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                           >  2:10947/1‑137 (MQ=255)

                                   ttCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAg                                                                                                        >  2:226533/1‑139 (MQ=255)

                                        cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  5:31731/1‑139 (MQ=255)

                                        cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  7:283001/1‑139 (MQ=255)

                                        cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCGCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  7:97142/1‑139 (MQ=255)

                                          cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  2:280041/1‑99 (MQ=255)

                                          cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  8:78804/102‑1 (MQ=255)

                                          cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCa                                                                                                 >  8:291699/1‑139 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTACGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACt                                                                                              >  4:197398/1‑139 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  5:251881/94‑1 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            >  3:57307/1‑109 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  8:243932/1‑138 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  7:148142/1‑138 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  4:222782/1‑138 (MQ=255)

                                                  ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                         >  5:163723/1‑139 (MQ=255)

                                                     gggctgggtGTTTTATTGCTTATAAATCTGGAGCGGGTGAGCGTGGGTCTCGCGGTATAATTGCAGCACTGGGGCCTTATGGTAAGCCCGCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  4:143078/138‑1 (MQ=255)

                                                     cggcggGCTGTTTTATTGATGATAAATGTGGCGCGGGTGAGGGGGGGTCTCGCGTTATCATTGCAGCTCTGGGGCCAGTTGGTAAGCCCGCCCGTATCGTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  7:239324/139‑1 (MQ=255)

                                                     cGGCTGGCTGGTTTATTGGTTATAAAGCTGGCGCCGGTGCGCGGGGGGGTCGGGGTATCTTTGCAGCCCTGGGGCCAGATGGTAAGCCCGCCCGTATCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  3:132062/139‑1 (MQ=255)

                                                      ggcgggcgGGTTTCTTGCTAATTAATCGGGCCCCGGGGCGCGTGGTTCGCGGGGTTTCATTGCCCCCCTGGGCCCTGATGTAAAGCCCTCCCGTAGCGTTGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGaa                                                                                     <  2:8570/139‑1 (MQ=255)

                                                         tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           >  2:163791/1‑82 (MQ=255)

                                                            ctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              >  4:267697/1‑92 (MQ=255)

                                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  3:180623/1‑137 (MQ=255)

                                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  3:293626/1‑137 (MQ=255)

                                                                             aaTCTGGCGCCGGGGCGGGGGGGTCTCGTGGAATCATGGCCCCCCTGGGCCCAGTTGGTAACCCCTCCCGTAGCGTAGTTAGCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                              <  5:183647/139‑1 (MQ=255)

                                                                                  ggAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCTACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTg                                                         >  6:149719/1‑139 (MQ=255)

                                                                                     gcgggTGTGCGTGGGGCTCGCGTTAACATTGCAGCAGTGGGGCCAGTTGGTACGCCCTCCCGTATCTTTTTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCt                                                      <  1:235422/136‑1 (MQ=255)

                                                                                      ccGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  5:204089/1‑58 (MQ=255)

                                                                                       cggggcgCGGGGGGCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCTTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  1:2548/133‑1 (MQ=255)

                                                                                       cGGTGAGCGTGGGCCGCGCGGTATCATTGCCCCACGGGGGCCAGATGGTACGCCCTCCCGTATCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  8:202948/139‑1 (MQ=255)

                                                                                        ggTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAc                                                   >  5:286364/1‑139 (MQ=255)

                                                                                         gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  7:76636/1‑139 (MQ=255)

                                                                                         gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  7:47112/1‑139 (MQ=255)

                                                                                         gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  1:280299/1‑139 (MQ=255)

                                                                                                        gcgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATAGCTGAGCTAGGTGCCTCACTGATTAAGCATTGGt                                    >  4:30758/1‑138 (MQ=255)

                                                                                                             aTCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                              >  6:274803/1‑139 (MQ=255)

                                                                                                                  tGCAGCACTGGGGCCAGATGGTTAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                         >  3:276849/1‑139 (MQ=255)

                                                                                                                  tGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATAGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                         >  6:175845/1‑139 (MQ=255)

                                                                                                                   gcagcaCTGGGGCCTGATGGTAAGCCCTCCCTTATGTTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                        <  3:37467/139‑1 (MQ=255)

                                                                                                                    cagcaCTGGGGCCAGATGGTAAGCCCTCCCTTTTCGTATTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                        <  6:253033/138‑1 (MQ=255)

                                                                                                                      gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  6:73880/1‑139 (MQ=255)

                                                                                                                        cgtGGGGCCTGAGGGCAAGCCCTCCCGATTTGTATTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   <  6:230527/137‑1 (MQ=255)

                                                                                                                        cggggggCCTGATGGTACCCCCTCCGGTAGCTTAGTTAGCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   <  4:288935/136‑1 (MQ=255)

                                                                                                                         cTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   >  8:185938/1‑138 (MQ=255)

                                                                                                                          tGGGCCCAGATTGTACCCCCTCCCGTATCTTATTAATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  2:256002/139‑1 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTCCACGTCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGTTGCCTCACTGATTAAGCATTGGAAACTGTCAGACCAAGTTTACt               >  5:232058/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  7:6391/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  3:97923/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  3:76717/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  7:128284/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  2:133617/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAAGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  8:42219/1‑139 (MQ=255)

                                                                                                                               ccAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  7:202821/99‑1 (MQ=255)

                                                                                                                               ccAGATGGTAAGCCCTCCCGTAGCTTAGTTAGCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCat            <  8:168690/139‑1 (MQ=255)

                                                                                                                               ccAGAGGGTACGCCCTCCCGTTTCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCat            <  6:51812/139‑1 (MQ=255)

                                                                                                                                 agtgggTAAGCCCTCCGTTTTCGTTGTTTTCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat          <  8:22303/135‑1 (MQ=255)

                                                                                                                                 aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat          >  4:83325/1‑139 (MQ=255)

                                                                                                                                   aTGTTAAGCCCTCCCGTATCGTAGTTATCTACAAGACTGGGAGTCAGGCAAATATGGATGACCGAAATAGACAGATCGATGAGATAGGTGCATAACTGATTAAGCATTGTTACCTTTCAGAGCAAGTATACTCatata         >  6:202286/1‑138 (MQ=255)

                                                                                                                                   aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         >  3:218563/1‑138 (MQ=255)

                                                                                                                                       tAAGCCCTCCCGTATCGTAGTTATCTACACGCCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    >  7:16821/1‑139 (MQ=255)

                                                                                                                                       tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  8:55376/57‑1 (MQ=255)

                                                                                                                                        aaGCCCTCCCGTATCGTAGATATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGCCAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    >  1:1000/1‑138 (MQ=255)

AGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  Exported/4488‑4763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`