BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,006	T→G	6.5%	V40G (GTT→GGT)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,006	0	T	G	6.5%	16109.4 / 207.5	6723	V40G (GTT→GGT)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (2965/3142); new base G (544/38); total (3529/3194)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6764 total aligned reads displayed.

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

aTTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGt                                                                                                                                             <  7:85486/139‑1 (MQ=255)

    cacacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                         >  5:50077/1‑139 (MQ=255)

     acacaGGAAACAGCTATGACCATGATTACGGCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACTACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                         <  3:270578/138‑1 (MQ=255)

         aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCACCGGGTACCGAGCTCGAATACACTGGCCGTCGTTTTACAACGTCCTGACTGTGTAAAACCACGCGATACCCaaat                                                                                                                                    >  5:86308/1‑137 (MQ=255)

           gAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTTGCGTTACCCAACtt                                                                                                                                   >  1:156217/1‑138 (MQ=255)

           gAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACtt                                                                                                                                   >  6:270887/1‑138 (MQ=255)

            aaaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                 <  8:1539/139‑1 (MQ=255)

             aaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                                <  4:170239/139‑1 (MQ=255)

                   tATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGGACCGAGCTCGAATTCACTGGCCGTCTTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGcc                                                                                                                            <  2:143072/137‑1 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCCACGTCGTGACTGGGGAAACCCGGGCGGTACCCCACTGAATCGCCGTgc                                                                                                                        >  1:85777/1‑139 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTCACCAACTTAATCGCCGTgc                                                                                                                        >  4:129948/1‑139 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                        >  1:172454/1‑139 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                 <  2:28576/128‑1 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATCCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTACCCCACTTAATCGCCTGgcag                                                                                                                      >  8:170925/1‑139 (MQ=255)

                          aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                         <  7:4733/117‑1 (MQ=255)

                            gaatACGCCAAGCTTGCATGCCTGCAGGTCGACTCAAGAGGATCCCCAGGAACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGAGAAAACCCAGCCGTTACCCAACATAATCGCCTTGCACcacaa                                                                                                                 >  4:145826/4‑138 (MQ=255)

                                 cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGGGCTGGGAAACCCCGTGCGTTACCCACCGTAAGCGCCTTGCAGCCCCTcccc                                                                                                             >  8:80208/1‑138 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                       >  8:44936/1‑139 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                       >  5:129351/1‑139 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                        >  2:42697/1‑122 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACGTAATAGCCGTGCAGCACATCCCCCATTCg                                                                                                       >  3:202938/1‑139 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATAGCCTTGCCGCACCTCCCCCGTTCg                                                                                                       >  6:231217/1‑139 (MQ=255)

                                       gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGCGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGACTGGGAAAACCCTGGCGGTACCCCACTTAATCGCCTTGCAGCACATCCCCCTTTCGc                                                                                                      >  4:193560/1‑139 (MQ=255)

                                           gCATGCCTGCAGGGCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCa                                                                                                    <  4:31289/137‑1 (MQ=255)

                                           agacagcTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  3:42568/133‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACATCGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  1:258872/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  5:173863/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  7:221891/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCCTCCCCACCCCCATTCGCCAg                                                                                                   >  2:108388/1‑137 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTAAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  1:100073/138‑1 (MQ=255)

                                                ccTGCAGGTCGACTCTAGAGGATCCCCGGGTTCCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCg                                                                                            <  7:242367/139‑1 (MQ=255)

                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTAAAACGTCCTGCCTGGGAAAACCCGGTCCTTACCCAACTTAATACCCTTTCCGCACAAACCCCGTTAGCCCGCA‑GGCgg                                                                                           >  6:10684/1‑137 (MQ=255)

                                                     aGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATa                                                                                       <  7:201157/139‑1 (MQ=255)

                                                     aGGTCGACTCTAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATa                                                                                       <  6:88349/139‑1 (MQ=255)

                                                       gTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATa                                                                                       <  6:157636/137‑1 (MQ=255)

                                                          gtctatAGAGGATCCCCGGGTACCTAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACGTAATCGCCTTGCAGCACATCCCCCTTGCGCCAGCT‑GGCGTAATAGAGaa                                                                                  <  2:187115/134‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCT‑GGCGGAATATCGa                                                                                   >  8:297949/1‑138 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGaa                                                                                  <  1:31160/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGaa                                                                                  <  3:151308/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGaa                                                                                  <  7:32520/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTAGAATTCACTGGCCGTCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGaa                                                                                  <  7:284277/139‑1 (MQ=255)

                                                          gACTCTAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGac                                                                                  <  1:123669/139‑2 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTTCCCCACATAATCGCCTTGCAGCACCTCCCCCATTCGCCAGCA‑GGCGTAATAGCGAag                                                                                 >  6:209933/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGCGCTCGAATTCACTGGCCGTCGGTTTTCAACGTCGTGACTGGGATAACCCTGGCGTTTCCCAACTTAAAAGCCGTGCCGCCCAACCCCCAATCGCCAGCG‑GGGGGAATTTCGGAGAGGcccccccc                                                                      >  3:222190/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCATGCCGCACAACCCCCATTCCACAGCA‑GGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  8:276382/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCGGGCGGTACCCAACGGAAACGCCGTGCCGCCCCACCCCCCTTAGCCAGCA‑GGCGGTGTAGCGCAGAGGCCCGcccc                                                                      >  6:254624/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCTTGCAGCACATCCCCCGTTATCCCGCT‑GGCGGAATAGCGAAGAGGcccccccc                                                                      >  2:259520/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCACCATAATCTCCTTGCAGCCAATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  8:252307/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                         <  3:90009/89‑1 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  8:60339/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGcccc                                                                      >  5:114424/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCGTTCGCCAGCG‑GGCGTAATTGCGAAGAGGCCCGcccc                                                                      >  1:23765/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGGAATAGGGAAGAGGcccccccc                                                                      >  1:280327/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAACCAACTTAATAGCCTTGCAGCACATACCCCTTTCGCCCGCT‑GGCGTTATAGCGAAGAGGCCCGCAcc                                                                      >  8:132332/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATAGCCGTGCAGCACATCCCCCATTCGCCCGCTGGGGGTAAT‑GCGAAGAGGCCCGCAcc                                                                      >  4:238054/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  7:263334/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGGGCTGGGAAAACCCTGGCGTGTCCCCACATAATCGCCTTGCAGCCCCGCCCCCTTTCGCCCGCG‑GGCGGTATAACGAAGCGGCCCGcccc                                                                      >  6:184147/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGTCTGGGCAAAACCAGGCGGTACCCAACTAAATAGCCGGTCAGCCCCACACCCATTAGCCCGCT‑GGCGGAATAACGAAGAGGcccccccc                                                                      >  4:56915/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGTATGGGAAAACCCTGGCGTTACCCCACTAAATCGCCATGCCGCACCTACCCCATTCGCCCGCT‑GGCGTAATAGGCAAGAGGCCCGcccc                                                                      >  4:261282/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCCTCCTTTTACAACGTCGTGACTGGGGCAACCCAGGCGTTACCCCACTTTAAAGCCTTGGAGCCCCTCCCCCATTCCCCAGCT‑TGCATCATAGCGAAGAAGCCCCCAcc                                                                      >  2:66295/1‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCg                                                                                            <  4:96707/116‑1 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTAGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCg                                                                     >  7:11123/1‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTAAAACGTCGTGACTTGGAAAACCCTGGCGGTTCCCAACATAATCGCCTTGCCGCACATCCCCCTTTCGCCAGCG‑GGCGTAAAAGCTCATAGGCCCCCAc                                                                       >  2:209167/1‑137 (MQ=255)

                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGAt                                                                   >  3:115929/1‑139 (MQ=255)

                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACGTAATCGCCTTGCAGCACATCCCCCATTCGCCAGCT‑GGCGGTATATCGAAGAGGCCCGCCCCCAt                                                                   >  2:23824/1‑139 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  1:215237/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  5:67857/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  8:101564/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  5:275524/139‑1 (MQ=255)

                                                                             ggaCCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  7:137082/137‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  2:170717/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  7:179530/139‑1 (MQ=255)

                                                                              tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGccc                                                              <  1:46943/139‑1 (MQ=255)

                                                                               aCCGAGCTCGAATTAACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTGACCCACCTTAATCGCCATGCAGCACATCCCCCTTTCGCCACCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGccc                                                              >  8:191078/1‑138 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTTACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                            <  5:229445/139‑1 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGGCTTACACAACTTAATCGCGTTGCAGCACATCACCCTTTCGCCAGCT‑GGCGTAATAGGGCAGAGGCCCCCCCCGCTCGcccag                                                            >  2:87919/1‑137 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                            <  3:66714/139‑1 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATACCCCTTTCCCCACCT‑GGCGAAATAGCCAAGAGGCCCCCCCCGATCGcccat                                                            >  7:159381/1‑137 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                <  5:295871/85‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTcc                                                          >  3:133943/1‑138 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTCGCCAGCT‑GGCGGAATAGCGAAGAGGCCCGCCCCGATCACCCTTccc                                                         >  5:149266/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCAACTTAAAAGCCTTGCAGCAAATCCCCCTTTCGCCAGCA‑GGCGGTATAGCGAACACGCCCCCCCCGAACCCCCGTcc                                                          >  8:210410/1‑138 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCCTGACTGGGCAAACCCTGGCGGTTCCCAACTTAATAGCCTGGCCGCCCCACCCCCATTCGCCCCCT‑GGCGTAATAGGGAAGAGGCCCGCCCCGCGCGcccctgcc                                                         >  4:283173/1‑134 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTAGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCGTGCAGCACCTCCCCCATTAGCCAGCA‑CGCCTAATAGCGAAGACGCCCGCCCCGATCGCCCTGccc                                                         >  6:67351/1‑139 (MQ=255)

                                                                                             cACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgc                                                <  1:65566/138‑1 (MQ=255)

                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  3:245077/139‑1 (MQ=255)

                                                                                                tGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGGAATAGCGAAGAGGCCCGCCCCCATCGCCCATCCCAACAGTTGCGCAg                                            >  5:254434/1‑139 (MQ=255)

                                                                                                tGGCCGTCGTTTTACAAAGTCGTGACTGGGAAAACCCTTGCGTTAAACAACATAATCGCCTTGCAGCAACTACCCCTTTAGACACCT‑GGCGTAATATCGACAAGGCCCGCACCGATAGCCCTTCCCAACAGATTCGCAg                                            >  8:23278/1‑139 (MQ=255)

                                                                                                 ggCCGTCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGACGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                           <  5:202029/139‑1 (MQ=255)

                                                                                                    cgtcgtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTg                                        >  3:179230/1‑139 (MQ=255)

                                                                                                     gtcgtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                       <  6:120997/139‑1 (MQ=255)

                                                                                                                   tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATa                                                                                       <  4:78906/77‑1 (MQ=255)

                                                                                                                      tGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGaa                                                                                  <  6:136824/79‑1 (MQ=255)

                                                                                                                           gggAAAACCCTGGCGTTACCCAACTTAATCGCCTCGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  1:193034/139‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  4:217431/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  3:288543/138‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCGGAATGGCGAATTGCGCCTGATGCgg                >  6:291106/1‑139 (MQ=255)

                                                                                                                                aaCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt            <  4:112795/139‑1 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  7:66642/139‑1 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGCGGCCCGCACCGATCGCCCTTCCCAACAGTTTCGCAGCCTGAAAGGCGCATGGCGCCTGATGCGGTATTTTGTcc      >  6:45182/1‑139 (MQ=255)

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT‑GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`