BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,031	A→C	6.7%	A48A (GCA→GCC)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,031	0	A	C	6.7%	19171.2 / 220.2	7201	A48A (GCA→GCC)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2881/3691); new base C (595/13); total (3491/3710)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 8111 total aligned reads displayed.

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169

 aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGcac                                                                                                                                            >  3:209804/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGCGTTACCCAACATAATCGCC‑GTGCAGCCCAt                                                                                                                                          >  2:181354/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGTAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCCGCACAt                                                                                                                                          >  1:64634/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACCCGGGCGTTACCCCACGTAATCGCCGTTGC‑GCACAt                                                                                                                                          >  2:38199/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACAt                                                                                                                                          >  4:264492/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACAt                                                                                                                                          >  5:299299/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCCATGCGGTGCCCAACGTAATAGCC‑TTGCAGCACAt                                                                                                                                          >  2:231772/1‑139 (MQ=255)

        cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTAGTGACGGGGAAAACCCAGGCGTTTCCCAACTGAATAGCC‑TTGCAGCACATccccc                                                                                                                                     >  3:87624/1‑139 (MQ=255)

        cGCCAAGCTTGCATACCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGTAAACCCTGGCGTTACCCAACTTAATCGCC‑GTTCAGCCCCTccccc                                                                                                                                     >  1:44749/1‑139 (MQ=255)

             atctagCATGCCTGCAGCTCGACTCTATAGGATCCCCTGAAACCGATCACGAATTCACTGGCCGTCGATTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAACCAACTTAATCGCC‑TTGCAGCCCATCCCACTTTCg                                                                                                                                >  8:244506/6‑139 (MQ=255)

             aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCAATGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCCTTACCCAACATAATACCC‑TTGCAGCCCATCCCCCATTCg                                                                                                                                >  7:201839/1‑139 (MQ=255)

              gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTTATCGCC‑TTGCAGCCCCTCCCCCGTTCGc                                                                                                                               >  3:252680/1‑139 (MQ=255)

              gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGc                                                                                                                               >  5:103084/1‑139 (MQ=255)

                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                           <  7:221891/138‑1 (MQ=255)

                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTAAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                           <  1:100073/138‑1 (MQ=255)

                         tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGTCTGGGCAAACCCAGGCGTTTCCCAACGTATTGGCC‑TTGCAGCACCTCCCCCATTAGCCAGCGGGCGt                                                                                                                     >  7:265447/1‑138 (MQ=255)

                         tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATc                                                                                                                                         >  5:257279/1‑118 (MQ=255)

                         tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGGTACCCAACATAATCGCC‑TTGCCGCACATCCCCCTTTCGCCAGCAGGCGt                                                                                                                     >  7:33435/1‑138 (MQ=255)

                         tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACCTCGTGACTGGGTAAACCCTGGCGTTACCCCACATAAACGCC‑ATGCAGCACATCCCCCATTACCCCGCAGGCGt                                                                                                                     >  6:259453/1‑138 (MQ=255)

                           cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACGGGGAAAACCCTGGCGTTACCCAACTTAATCCCC‑ATGCACCAAAGGCCCCTTTCGCCAGCAGGccgaa                                                                                                                   >  8:36137/1‑134 (MQ=255)

                            aGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATa                                                                                                                 <  1:252298/139‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                            <  4:218710/139‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTAACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                            <  6:159128/139‑1 (MQ=255)

                                 gACTCTAGAGGAGCCCCGGGGACCGCGCTCGAATTCACTGGCCGGCGTATTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTACTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGGAATAGCGaa                                                                                                            <  5:68328/139‑1 (MQ=255)

                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCg                                                                                                              >  3:295108/1‑136 (MQ=255)

                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCATGCGTGTCCCAACTTAATAGCC‑TTGCATCCCCTCCCCCATTAGCCACCTGGGGTTATAggc                                                                                                              >  5:184444/1‑134 (MQ=255)

                                         agGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑g                                                                                                   <  3:43150/139‑1 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTTGGTAAAACCTGGCGGTAACCCACATAAAAGCC‑GTTCAGCACCAACCCCATTAGCCCCCGGGCCTAATAGCGAAGAGGcccccccc                                                                                                >  4:173944/1‑134 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCAcc                                                                                               >  7:49284/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTAAACCCTGGCGTTACCCAACTTAATCGCC‑ATGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCAcc                                                                                               >  6:75784/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGc                                                                                                                               <  3:128442/108‑1 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                           <  6:107222/112‑1 (MQ=255)

                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGCAAACCCTGGCGTTACCCAACTTAAACGCC‑TTGCAGCCCATCCCCCGTTCGCCCGCTGGCGTAATAGCGAAGAGGCCC‑GCCCCg                                                                                              >  4:58156/1‑139 (MQ=255)

                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGGAATAGCGAAGAGGCCC‑GCACCg                                                                                              >  8:53985/1‑139 (MQ=255)

                                                ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTAAAAAGTCCTGACAGGGAAAAACCATGCGTTACCCAACATTATACCC‑TTTCACCACAAACCCCGATCGCCATCTGGCGGAATATCCAAGATGCCC‑CCAccca                                                                                             >  4:24228/1‑136 (MQ=255)

                                                  gggTACCGAGCTCGACTTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCg                                                                                          <  4:109601/139‑1 (MQ=255)

                                                  gggTACCGAGCTCGAATTCACTGGGCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAAATTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCg                                                                                          <  5:281964/139‑1 (MQ=255)

                                                  gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCg                                                                                          <  1:276522/139‑1 (MQ=255)

                                                  gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCg                                                                                          <  4:285403/139‑1 (MQ=255)

                                                    gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGcc                                                                                        <  1:120443/139‑1 (MQ=255)

                                                    gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGACTGCGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCCGGCGTAATAGCCAAGAGGCCC‑GCACCGATCGcc                                                                                        <  8:265037/139‑1 (MQ=255)

                                                       ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGGAAACCCGGGCGGTACCCCACGTTATAGCC‑GTGGAGCCCCACCCCCGTTCGCCCGCAGGGGGAATAGCGCAGCGGCCCGGCACCGAGCGcccg                                                                                      >  2:60208/1‑138 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAACACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCCCCTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCCCCGATCGCCCTTccc                                                                                  >  8:156845/1‑139 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTccc                                                                                  <  3:66571/139‑1 (MQ=255)

                                                              cGAATTCACTGGCCGTCGTTTTACAACGTCGTTACTGGGAAAACCCTCGCGTTAACCAACTTAAAAGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGAACGCCCATCccacca                                                                              >  7:243518/1‑139 (MQ=255)

                                                                 aTTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTTCCCAACGTAATCGCC‑GTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCC‑GCCCCGCTCGCCCTTCCCCACCGtt                                                                           >  1:166029/1‑139 (MQ=255)

                                                                 aTTCACTGGCCGTCGTTTTACAACGTAGATACGGGGAAAACCCTGGCGTTTCCCAACTTAAAACCC‑GTGGAGCACCTCCCCCTTTCCCAACCAGGCCTAATTGCGACAAGGCAC‑CCCCCGCTCGCCCATCCCCAccctt                                                                           >  6:138419/1‑135 (MQ=255)

                                                                    cACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTgc                                                                         <  4:148665/138‑1 (MQ=255)

                                                                     aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  7:163350/139‑1 (MQ=255)

                                                                     aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  7:88641/139‑1 (MQ=255)

                                                                         gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATCGCC‑TTGCAGCCCATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCGTCCCCCCAGTTGCGCAGcc                                                                   >  7:70139/1‑139 (MQ=255)

                                                                         gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGcc                                                                   >  1:29814/1‑139 (MQ=255)

                                                                            gtcgtTTTACAACGTCGGGCCTGGGAAAACCCAGGCGTTACCCAACTTAATCGCC‑TTCCAGCACATCCCCCTTTCGCCAGCTGGCGTAAAAGCGAAGAGGCCC‑GCACCTATCGCCCTTCCCCACAGTTGCGCAGCCTga                                                                <  6:298607/139‑1 (MQ=255)

                                                                            ggcgtTTTACACCGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                                                <  2:135139/137‑1 (MQ=255)

                                                                               gtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGGAATAGCGAAGAGGCCC‑GCCCCGATCGCCCATCCCCACAGTTGCGCAGCCTgaatg                                                             >  1:20971/1‑139 (MQ=255)

                                                                               gtTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTAACCAACGTAATAGCC‑GTGCAGCCCCTCCCCCATTCGCCAGCAGGCGTAATAGCGCAGACGCCC‑CCACCCCTCCCCCCTCCCCACAGTTGGGCACCCGgaatg                                                             >  2:280016/1‑139 (MQ=255)

                                                                                     cAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                                                       <  1:14665/139‑1 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                  >  5:52540/1‑139 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAAACGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTTGCGTAAAAGCGAATAGGCCC‑GCACCGATCGCCCATCCCCACCGTTGAGCAGCCTGAATGGCGAATTgcgc                                                  >  8:133951/1‑139 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCGGGCGGTTCCCAACTTTATCGCG‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGCAGCGGCCC‑GCACCGATCGCCCTGCCCAACAGTTGCGCGGCCTGAAAGGCGAATGgcgc                                                  >  2:161886/1‑139 (MQ=255)

                                                                                             tGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCt                                                >  8:1056/1‑138 (MQ=255)

                                                                                                   ggAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCTGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACa                                                                              <  6:21522/102‑1 (MQ=255)

                                                                                                   ggAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                           <  4:131332/137‑1 (MQ=255)

                                                                                                   ggAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                          <  3:275892/138‑1 (MQ=255)

                                                                                                    gAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  3:144237/108‑1 (MQ=255)

                                                                                                     aaaaCCCTGGCGTTACCCCACTTAATAGCC‑TTGCAGCCCAAACCCCTTTCGCCCGCGGGCGGTATAGCGAAGAGGCCC‑GCCCCGCTCGCCCGTCCCCACACTTGCGCCGCCGGAATTGCGGATGGCGCCGGGTGCGGTa                                       >  4:32311/1‑139 (MQ=255)

                                                                                                         cccTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                   <  3:190173/139‑1 (MQ=255)

                                                                                                           cTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                 <  4:197835/139‑1 (MQ=255)

                                                                                                           cTGGAGTTACCCAACGTACTCGCC‑TTGCAGCACATCCACCTTTCGCCAGCTGGCTTAATAGCGAAGATGCCC‑GCACCGATCGCCCTTCCAAACAGTTGCGCAGCCTGAAGGGCGAATGGCGCCTGATGCGGGATTTtct                                 <  1:229061/139‑1 (MQ=255)

                                                                                                              gCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCt                              >  5:85696/1‑139 (MQ=255)

                                                                                                              gCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCt                              >  5:208422/1‑139 (MQ=255)

                                                                                                                gTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                            <  5:137570/139‑1 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTTAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          >  6:189138/1‑138 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          <  2:255964/138‑1 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          >  4:81774/1‑138 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGAGCAGCCTGAATGGCGAATTGCGCCTGATGCGGTATTTTCTCCTTACg                          >  5:11716/1‑138 (MQ=255)

                                                                                                                    cccAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                 >  7:182985/1‑130 (MQ=255)

                                                                                                                        aCTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtg                    <  5:233454/139‑1 (MQ=255)

                                                                                                                         cTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCaa                                                                                >  2:83532/1‑78 (MQ=255)

                                                                                                                         cTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGc                         <  7:285358/133‑1 (MQ=255)

                                                                                                                         cTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGc                         >  8:285358/1‑133 (MQ=255)

                                                                                                                         cTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCCTCtgt                   >  2:204381/1‑139 (MQ=255)

                                                                                                                         cTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                   >  6:23637/1‑139 (MQ=255)

                                                                                                                            caTCGCC‑TTGCAGCACATCCCCCTTTCGCCCGCGGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  7:120164/136‑1 (MQ=255)

                                                                                                                            aaTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  6:55672/137‑1 (MQ=255)

                                                                                                                            aaTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  6:281818/137‑1 (MQ=255)

                                                                                                                            aaTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  6:121758/137‑1 (MQ=255)

                                                                                                                            aaTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  1:184731/137‑1 (MQ=255)

                                                                                                                             aTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                <  3:228179/138‑1 (MQ=255)

                                                                                                                             aTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                <  8:176948/138‑1 (MQ=255)

                                                                                                                             aTCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGGTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                <  7:14204/138‑1 (MQ=255)

                                                                                                                              tCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCgg               >  3:165363/1‑138 (MQ=255)

                                                                                                                                gCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTa             <  8:198532/138‑1 (MQ=255)

                                                                                                                                 cc‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGATGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAtt           >  1:857/1‑139 (MQ=255)

                                                                                                                                       cagccCATCCCCCTTTCGCCAGCTGGCGTAATAGCCACGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGACTGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcaca      <  3:108193/139‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  6:96215/138‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGACGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  5:158534/138‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  8:221500/138‑1 (MQ=255)

                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  8:7643/138‑1 (MQ=255)

                                                                                                                                        agcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCacac     <  2:104024/139‑1 (MQ=255)

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC‑TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCC‑GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`