| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 900,276 | C→T | 7.3% | A66V (GCG→GTG) | yfiG → | putative sugar/proton transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 900,276 | 0 | C | T | 7.3% | 169.2 / 3.4 | 55 | A66V (GCG→GTG) | yfiG | putative sugar/proton transporter |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base T (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
TTTACGAACGATTACATTGGTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTGGCTGTACCTTTTCAC > BS168_reference_genome/900139‑900410 | tttACGAACGATTACATTGGTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGc > 5:28379/1‑138 (MQ=255)tttACGAACGATTACATTGGTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCg > 8:168268/1‑139 (MQ=255) tACGAACGATTACATTGGTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGa < 3:270666/138‑1 (MQ=255) acgaacgaTTACATTGGTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAg < 1:216625/138‑1 (MQ=255) cATTGGTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTtct < 2:282341/139‑1 (MQ=255) gTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCgggg > 7:128357/1‑139 (MQ=255) tCTACATTCGGAGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTTAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCa < 7:51624/138‑1 (MQ=255) aCATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCt > 3:62010/1‑139 (MQ=255) tCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCgg > 7:57089/1‑139 (MQ=255) gcggcCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGc < 6:131872/139‑1 (MQ=255) cggcCTGGAGTTCGCGAATGCTACGCGTGTCCTAAATGGGGCACTTCCGTTTCTGGCACCTGCAGGTCAGCTGAATCTTACACCTGTTACAGAACGTCTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCg < 6:42507/139‑1 (MQ=255) ttgggTTATGATACGGTTGTCATTAATGGGGCACTTGCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTggag < 4:62010/136‑1 (MQ=255) cGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCg < 4:14030/138‑1 (MQ=255) cGGGTGTCATTAAGGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCg < 6:107289/138‑1 (MQ=255) ggTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATc > 3:119951/1‑139 (MQ=255) ggTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATc > 6:221294/1‑139 (MQ=255) aTTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCg < 3:215714/108‑1 (MQ=255) aTTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACg > 8:13274/1‑139 (MQ=255) aTTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACg > 2:147181/1‑139 (MQ=255) aTTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCGTCGGGGCg > 4:215714/1‑108 (MQ=255) tGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGgccgc > 7:252866/1‑139 (MQ=255) tGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTATCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGgccgc > 6:66303/1‑139 (MQ=255) ggCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCtt > 5:122036/1‑93 (MQ=255) ggCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCtt < 6:122036/93‑1 (MQ=255) cACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGTCACGGCCGCCGaa > 8:94278/1‑138 (MQ=255) cACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGaa > 8:118434/1‑138 (MQ=255) cACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGaa > 5:139872/1‑138 (MQ=255) ttATGGCACCTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGGCTGTCCGATCGGCACGGCCGCCGAAAAACGATc < 3:189428/137‑1 (MQ=255) ttATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATc < 6:28379/137‑1 (MQ=255) ttATGGCAACTGCAGGTCAGCTGAATCTTACACCTGGTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATc < 5:221294/137‑1 (MQ=255) tATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTGCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCt < 5:242549/138‑1 (MQ=255) gCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCtttatt < 4:119951/139‑1 (MQ=255) gCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCtttatt < 1:147181/139‑1 (MQ=255) gCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCtttatt < 4:219244/139‑1 (MQ=255) cTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCGTTATTTAGCCTTACTCTTc > 5:4916/1‑138 (MQ=255) aaTCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCAt < 7:168268/137‑1 (MQ=255) acacCTGTTACAGAAGGACTGGTTGCGAGGTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGcc < 8:128357/138‑1 (MQ=255) acacCTGATACAGAAGGACTGGTTGTGAGTTGCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGc < 8:190992/137‑1 (MQ=255) acCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCcaca < 7:13274/139‑1 (MQ=255) cTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGtt < 6:155737/61‑1 (MQ=255) cTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGtt > 5:155737/1‑61 (MQ=255) gTTACAGAAGGACTGGTTGTGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGc < 8:75872/131‑1 (MQ=255) gTTACAGAAGGACTGGTTGTGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGc > 7:75872/1‑131 (MQ=255) gTTACAGAAGGACTGGTTGTGAGTTCCCTGCTTCTCGGGGAAGCCTTCGGGGCGAAGTTTCGAGGACGTCTGTCCGCTCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCATACTCTTCATCGCAGCCACACTTg > 7:190992/1‑139 (MQ=255) gTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACTGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTg > 5:147902/1‑139 (MQ=255) gTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACg < 5:6939/99‑1 (MQ=255) gTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACg > 6:6939/1‑99 (MQ=255) gTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTg > 6:82853/1‑139 (MQ=255) gTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTg > 3:72533/1‑139 (MQ=255) gTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTg > 8:204792/1‑139 (MQ=255) gACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTGGCTGTACCtt < 3:19520/139‑1 (MQ=255) gACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTGGCTGTACCtt < 8:57089/139‑1 (MQ=255) gACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTGGCTGTACCtt < 6:139872/139‑1 (MQ=255) gTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACg < 1:242424/84‑1 (MQ=255) gTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACg > 2:242424/1‑84 (MQ=255) gTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTGGCTGTACCTTTTCAc > 2:171874/1‑139 (MQ=255) | TTTACGAACGATTACATTGGTATCTACATTCGGCGGCCTGTTGTTCGGTTATGATACGGGTGTCATTAATGGGGCACTTCCTTTTATGGCAACTGCAGGTCAGCTGAATCTTACACCTGTTACAGAAGGACTGGTTGCGAGTTCCCTGCTTCTCGGGGCAGCCTTCGGGGCGATGTTTGGAGGACGTCTGTCCGATCGGCACGGCCGCCGAAAAACGATCCTTTATTTAGCCTTACTCTTCATCGCAGCCACACTTGGCTGTACCTTTTCAC > BS168_reference_genome/900139‑900410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |