| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 966,494 | G→C | 26.1% | A59G (GCG→GGG) | ygaO ← | putative integral inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 966,494 | 0 | G | C | 26.1% | 40.3 / 2.7 | 29 | A59G (GCG→GGG) | ygaO | putative integral inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (9/12); new base C (4/3); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATATATGACGCGCGACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCAACAAACAGAATAAAGGGAGTGAGGTTTAAATCAAATACCATCCCGCCGCTCTTTGTGCCGAAAAAAGATGCTATGCCTTCTGAGGCTTCAAAGTATGAAATCAGCGCCCAGCATGCAAGGCCA > BS168_reference_genome/966366‑966628 | cGATATATGACGCGCGACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCTGGGCGGCTGTTTTTAGG‑TTAGGTGAAAAAAACCGCCGGcggcgc > 1:67664/1‑139 (MQ=255) ttaCGCGCGACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGATTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCaacaaac < 7:24062/137‑1 (MQ=255) tgccgcgcgACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCaacaaac < 6:285233/136‑1 (MQ=255) tGACGCGCGACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCaacaaac < 5:107417/139‑1 (MQ=255) gACGCGCTACATGCTTTTGCCTTCATCATTTGCTCTCTTTCATCCTGATCTTCAAAATCATAAGGCAGAAGTAGCTGCTTTCTGGAAGGGCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCaacaaac < 3:183937/138‑1 (MQ=255) gACGCGCGACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTCTG‑TAAGTAAAAATAAACCGCAGACGCCGCaacaaac < 2:5618/138‑1 (MQ=255) cgcgcgACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTCTG‑TTAGTACAAATAAACCCCAGACGCCGCaacaaacat > 3:132358/1‑137 (MQ=255) cgcgcgACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTATG‑GTAGTTAAAATAAAACGCACACGCCCCcacaaacca > 4:103417/1‑136 (MQ=255) cgcgcgACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTAGG‑TTAATACAAATAAAACCCACACGCCGCcaccaaaac > 4:15290/1‑135 (MQ=255) cgcgACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTGTT‑TTATTACAAAATAAACGCAAAAGCCCCAACAAACaaaa > 7:111547/1‑138 (MQ=255) gcgACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCGTTTTTTCTG‑TATGTACAAATAAACCGCAGCCGCCGCCACAAACCGAAt > 5:300821/1‑138 (MQ=255) gcgACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGAAGAAGTAGCTGCTTTCTGGCAGGGCGGCGTTTTTTATG‑TAAGTACAAATAAACCACAGACGCCGCCACAAAAAAAAt > 8:24062/1‑138 (MQ=255) tttGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCTTCAGGCAGAAGTAGCTGCTTTCTGGCTGGGCGGCTTTTTTTCTG‑TAAGTACAAATAAACCCCCGACGCCGCAACAAACAGAATAAAGGGAGTg < 3:15290/138‑1 (MQ=255) ggTCATCTTCTGCTCTCTTTAATCCTGCTCTTCAAACTCATCAGGCCGACGTAGCTGCTTTGTGGCAGGGCGGCTTTTTTTCTG‑AAAGTACAATTAAACCGCAGACGCCGCAACAAACAGAATAAAGGGAGTGAGGttt < 8:77928/138‑1 (MQ=255) cgacatcatCTGCTCTCTTTCATCCTGATCTTAAAACTCATCTGGAACATGTCGCGGCTTTATGGCCGGGCGGTTTTTTTTCTT‑TAATAACAAATAAACCCCAGACGCCGCAACAAACAGAATAAAGGGAGTGAGGttt < 2:271666/136‑1 (MQ=255) atcatcTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCGTTTTTTATG‑TAATTACAAAAAAACCCCAGACCCCCCACCAAACACAAAAAAAGGAGTGAGGtgttaa > 1:55577/1‑133 (MQ=255) cTGATCTATTTCATCAGGCGCTACAAACTCATCTGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTCTG‑TAAGTAAAAATAAACCGCCGACGCCGCAACAAACAGAATAAAGGGAGTGAGGTTTAAATCaaa < 8:111547/138‑1 (MQ=255) ctctctTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCGTTTTTTGTGTTATGTA‑AAATAAACCGCCGACGGCGCCACAAACAGAATAAAGGGTGTGGGGTTTAATTAAAATAc > 2:102240/1‑138 (MQ=255) tatttCATCGTGCTCTTCAAACTCATCAGGCACAAGTAGCGGCTTTCTGGCTGGGCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCACACGCCGCAACAAACAGAATAAAGGGAGTGAGGTTTAAATCAAATACCa < 5:155630/135‑1 (MQ=255) ccatccATCCTCCTCTACAACCACATCCGCCAGAAGTCGCTGCTTCGGGGCTGGGCGGCTTTTTTTCTG‑TATGACAAAATAAACCGCCCACGCCGCAACAAACAGATTAAAGGGAGGGAGGTTAAAATCAAATACCa < 2:67664/132‑1 (MQ=255) tCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTGTTTTTGTG‑TAATTACAAAAAAACCCCAGGCCCCCCAACAAACAGAATAAAAGGAGTGAGGTTTAAATAAAATACCAACCcgccgc > 4:209276/1‑139 (MQ=255) tCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCGTTTTTTGTG‑TTAGTAAAAAAAAACCGCCGACGCCGCAACCAACACAAAAAAGGGCGTGGGGTTTAAATAAAAAACCAACcagcccc > 6:78668/1‑137 (MQ=255) atcaGGCAGATGGAGGTGGTTTCTGGCAGGGGGTCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCAACAAACAGAATAAAGGGAGTGAGGTTTAAATCAAATACCATCCCGCCGCTCTTTGTGCCGaaaaa < 2:55577/138‑1 (MQ=255) ctggtgctgcgTTCTGGGGGGGGGGTTTTTTTTGTT‑AAATTAAAAAAAACCCCCCCACGCCGCAACAAACAGAATAAAGGGATTGAGGTTTAAATCAAATACCATCCCGCCGCTCTTTGTGCCGAAAAAAGATGCTAt < 2:119689/133‑1 (MQ=255) aGTAGCTGCTTTCTGGCAGGGCGGGTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCAACAAACAGAATAAAGGGAGTGAGGTTTAAATCAAATACCATCCCGCCGCTCTTTGTGCCGAAAAAAGATGCTATg < 3:103417/138‑1 (MQ=255) tttCTGGCAGGGCGGCGTTTTTTCGG‑GTAGTACAAATAAACCGCCGACGCCGCCACCAACAGAATAAAGGGTGTGAGGTTTAAATCAAATACCCTCCCGCCCCGGTTTGTGGCGGAAAAATAAGCGATGGCGTCGGAgg > 2:69201/1‑139 (MQ=255) gggCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCAACAAACAGAATaa < 5:296297/58‑1 (MQ=255) gggCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCAACAAACAGAATaa > 6:296297/1‑58 (MQ=255) aCCGCAGACGCCGCAACAAACAGAATAAAGGGAGTGAGGTTTATATCAAATACCATCCCGCCGCTCTTTGTGCCGAAAAAAGATGCTATGCCTTCTGAGGCTTCAAAGTATGAAATCAGCGCCCAGCATGCAAGGCCa > 3:56163/1‑138 (MQ=255) | CGATATATGACGCGCGACATGCTTTTGCCGTCATCATCTGCTCTCTTTCATCCTGCTCTTCAAACTCATCAGGCAGAAGTAGCTGCTTTCTGGCAGGGCGGCTTTTTTTCTG‑TAAGTACAAATAAACCGCAGACGCCGCAACAAACAGAATAAAGGGAGTGAGGTTTAAATCAAATACCATCCCGCCGCTCTTTGTGCCGAAAAAAGATGCTATGCCTTCTGAGGCTTCAAAGTATGAAATCAGCGCCCAGCATGCAAGGCCA > BS168_reference_genome/966366‑966628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |