Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,349,495	G→T	100%	A325A (GCC→GCA)	pitB ←	putative low‑affinity inorganic phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,349,495	0	G	T	100.0%	175.8 / NA	51	A325A (GCC→GCA)	pitB	putative low‑affinity inorganic phosphate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/35);  total (16/35)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCGAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAC  >  BS168_reference_genome/1349360‑1349626                                                                                                                                        |                                                                                                                                    ttCGAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCg                                                                                                                                  <  7:199406/139‑1 (MQ=255)      aTCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTAGAGAGATCCGGATGGTTTTCCTGTTCCATAAAAAAAACCGCTCATTTTACAGAAGCGGTGTGACATTAAAATATCAGATTTAAAATAAAGTATGCGagtg                                                                                                                              >  2:105189/1‑135 (MQ=255)            cacaTCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAg                                                                                                                       >  1:114137/1‑139 (MQ=255)             acaTCTGAATTAGGTTCTGATTATTGTTTTCGAGATATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAgt                                                                                                                      <  2:114137/139‑1 (MQ=255)                    aaaTAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAGCCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt                                                                                                                >  3:279606/1‑138 (MQ=255)                    aaaTAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTATCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt                                                                                                                <  4:227760/138‑1 (MQ=255)                       tAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGaaa                                                                                                            >  8:292928/1‑139 (MQ=255)                         ggTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATc                                                                                                          >  5:193851/1‑139 (MQ=255)                         ggTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGATCCTTTGACAGAAGCGGTGTGCCATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCTCTGAAATc                                                                                                          >  1:254402/1‑139 (MQ=255)                           ttCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCg                                                                                                         >  2:268748/1‑138 (MQ=255)                             ctgctgATTGTATTCGAGAGATCCGGAGGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                      <  6:23797/139‑1 (MQ=255)                             cTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt                                                                                                                >  1:234117/1‑129 (MQ=255)                              tGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTg                                                                                                               >  5:245567/1‑129 (MQ=255)                                cTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGaa                                                                                                      <  1:291705/136‑1 (MQ=255)                                   aTTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtg                                                                                                 <  3:44560/138‑1 (MQ=255)                                    ttGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                               <  6:245567/139‑1 (MQ=255)                                     tGTATTCGTGAGTTCCGGATGTTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAATCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                               <  6:180383/138‑1 (MQ=255)                                     tGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                               <  1:268748/138‑1 (MQ=255)                                       tATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgatga                                                                                             <  6:128369/138‑1 (MQ=255)                                       tATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgatga                                                                                             <  2:7795/138‑1 (MQ=255)                                       tATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgatga                                                                                             <  8:283326/138‑1 (MQ=255)                                         ttCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAcc                                                                                           <  4:264860/138‑1 (MQ=255)                                          tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCa                                                                                         >  3:209754/1‑139 (MQ=255)                                                 aTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                               <  8:117338/126‑1 (MQ=255)                                                 aTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat                                                                                               >  7:117338/1‑126 (MQ=255)                                                 aTCCGGATGGTTTACCTGTTCAATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGATGAAATCGCAAGCGTTATGCCCCATCTAAt                                                                                   >  7:164343/1‑138 (MQ=255)                                                     ggATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCa                                                                              <  4:170645/139‑1 (MQ=255)                                                      gATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCAcc                                                                                                                          <  5:233101/94‑1 (MQ=255)                                                      gATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCAcc                                                                                                                          >  6:233101/1‑94 (MQ=255)                                                          gTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCg                                                                          <  6:134757/138‑1 (MQ=255)                                                           ttttCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAACTCGGAAGCGTGATGACCCATGTAATGAGCATTCGCt                                                                        <  5:134175/139‑1 (MQ=255)                                                           ttttCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCt                                                                        <  7:292928/139‑1 (MQ=255)                                                              tCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTgg                                                                     >  1:250589/1‑139 (MQ=255)                                                                cTGTTCCATGAAAAAAACCTCTGCTTTGACAGAAGCGGTGTGACATTAAAATTTGATTTTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACGCATGTAATGAGCATTCGCTTGGcc                                                                   <  8:164343/139‑1 (MQ=255)                                                                   ttCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCg                                                                  <  4:209754/137‑1 (MQ=255)                                                                      cATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAccc                                                             <  6:193851/139‑1 (MQ=255)                                                                      cATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAccc                                                             <  1:140764/139‑1 (MQ=255)                                                                      cATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAcc                                                              <  7:28290/138‑1 (MQ=255)                                                                                       cTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTa                                             <  2:1236/138‑1 (MQ=255)                                                                                          tGACAGAAGCGGTGTGACATTAAAATATAATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcg                                           <  1:197038/137‑1 (MQ=255)                                                                                           gACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcgcg                                         <  7:229743/138‑1 (MQ=255)                                                                                                 aGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTAGGCCGTCCCCCAGTTTACGCCTTTTACGCGGTgggcc                                  >  2:294953/1‑137 (MQ=255)                                                                                                            aTTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGcc                       <  1:11907/139‑1 (MQ=255)                                                                                                                       atatTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATtgatg            <  1:294953/139‑1 (MQ=255)                                                                                                                             aaaaTAAAGTCTGCGAGGGCACCAAGTGTCGCTGAAATCGGAAGCGGGATGACCCATGTACTGAGCATGCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATgaagaa       <  2:138706/138‑1 (MQ=255)                                                                                                                                 tAAAGTCTGAGATGGCACCTAGTGTCTCTGAAATCGGAAGCGTGATGACCCATGTATTGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAc  <  1:127641/139‑1 (MQ=255)                                                                                                                                 tAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAc  <  5:192764/139‑1 (MQ=255)                                                                                                                                 tAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAc  <  3:24074/139‑1 (MQ=255)                                                                                                                                 tAAAGTATGCGAGGGCACCAAGTGGCGCTGAAATCGGACGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAc  <  6:180546/139‑1 (MQ=255)                                                                                                                                    aGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAccc                                                                                          >  7:120957/1‑48 (MQ=255)                                                                                                                                    aGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAccc                                                                                          <  8:120957/48‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TTCGAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGAC  >  BS168_reference_genome/1349360‑1349626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑