Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,474,890 C→T 100% L45L (TTG→TTA)  kre ← regulator of transcription factor ComK function via modulation of mRNA stability

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,474,8900CT100.0% 173.5 / NA 52L45L (TTG→TTA) kreregulator of transcription factor ComK function via modulation of mRNA stability
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/29);  total (23/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCG  >  BS168_reference_genome/1474765‑1475019
                                                                                                                             |                                                                                                                                 
ggAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATATTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGttttt                                                                                                                      >  5:277755/1‑139 (MQ=255)
ggAGGCATGTGAAAATACAAGTCTCCGATTGAGATAAGCACGACCGAATGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTAATTTCTTTCATTTACCTACTTTGACAAGCTTTTGTAAAGCACgagttt                                                                                                                      >  1:267724/1‑134 (MQ=255)
    gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                  <  1:170611/139‑1 (MQ=255)
     cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                 <  7:116866/139‑1 (MQ=255)
     cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                 <  4:234908/139‑1 (MQ=255)
       tgtgAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                  >  7:133698/1‑136 (MQ=255)
       tgtgAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTCTTGAAAAATGAAGCCCTAGTTTCTTTCCTTTACCTTCTTTGCCAAGCTTTTGTAAAGCCCGTTTTTTcagcag                                                                                                               >  2:285948/1‑139 (MQ=255)
         tgAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTT‑AGCAGata                                                                                                            <  4:79988/139‑1 (MQ=255)
            aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                          <  8:103190/139‑1 (MQ=255)
            aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                          <  4:13066/139‑1 (MQ=255)
            aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                          <  1:244268/139‑1 (MQ=255)
             aaTAGTAGTCTCCGATTGAGATCAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                         <  3:16794/139‑1 (MQ=255)
                    gtCTCCTATTGAGATAAGCTCGTCCGATTGCAGTTGTCTGAAGCATGTTTTTTTTTAAAACTGAAGCAGTATTTTATTTCCTTTACCTTATTTGACAATCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAttt                                                                                                   <  4:184202/138‑1 (MQ=255)
                       tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAGGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAggg                                                                                               >  4:279597/1‑139 (MQ=255)
                                 aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACACGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCgg                                                                                     >  8:6047/1‑139 (MQ=255)
                                 aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCgg                                                                                     >  2:53618/1‑139 (MQ=255)
                                 aTAAGCACGTCCGATTGCTGTTGGCTGAACCGTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACACGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCgg                                                                                     >  6:29441/1‑139 (MQ=255)
                                         gtacGATTGCTGTTGGCTGAAGCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTAGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATTTAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                             <  2:267724/136‑1 (MQ=255)
                                         gTCCGATTGCTGTTGGCTTAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGTGGGCGGTTTTTGCa                                                                             <  1:193886/139‑1 (MQ=255)
                                          tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTTCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAGATAGGTATTTAGGGTTGGGGGCGGTTTTTGCAt                                                                            >  4:61106/1‑139 (MQ=255)
                                          tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTca                                                                                                                   >  8:143433/1‑100 (MQ=255)
                                          tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTca                                                                                                                   <  7:143433/100‑1 (MQ=255)
                                          tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCAt                                                                            >  6:26270/1‑139 (MQ=255)
                                           ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGt                                                                                              >  4:71321/1‑120 (MQ=255)
                                           ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGt                                                                                              <  3:71321/120‑1 (MQ=255)
                                                tGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGt                                                                      >  1:8565/1‑139 (MQ=255)
                                                  cTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc                                                                    <  1:120028/139‑1 (MQ=255)
                                                  cTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc                                                                    <  6:277755/139‑1 (MQ=255)
                                                   tGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTCCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGGTTGGCGGTTca                                                                   >  2:108518/1‑139 (MQ=255)
                                                     ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACGTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                   >  6:225798/1‑137 (MQ=255)
                                                     ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcaca                                                                 <  5:225798/139‑1 (MQ=255)
                                                     ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                  <  8:190912/138‑1 (MQ=255)
                                                     ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                   <  1:53618/137‑1 (MQ=255)
                                                     ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                   >  4:2522/1‑137 (MQ=255)
                                                       ggCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAgt                                                               >  8:75257/1‑139 (MQ=255)
                                                       ggCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAgt                                                               >  8:179081/1‑139 (MQ=255)
                                                             aCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAAc                                                         >  5:121845/1‑139 (MQ=255)
                                                                 tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                          >  2:93321/1‑86 (MQ=255)
                                                                 tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                          <  1:93321/86‑1 (MQ=255)
                                                                  ggATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGc                                                      <  2:8565/137‑1 (MQ=255)
                                                                                 tGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTa                                     <  3:2522/139‑1 (MQ=255)
                                                                                 tGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTa                                     <  6:151551/139‑1 (MQ=255)
                                                                                     gCCCTATTTTCTTTCCTTTACCTTATTTGACAAGCTTTTGTAAAGCCCGGTTTTTCAGCAGATATAGGTATTTATGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGt                                 <  5:33519/139‑1 (MQ=255)
                                                                                                tttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                  <  6:258680/95‑1 (MQ=255)
                                                                                                tttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                  >  5:258680/1‑95 (MQ=255)
                                                                                                  tcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGa                       <  6:238417/136‑1 (MQ=255)
                                                                                                    cTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGGTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTCGGCTGCAGATCttt                  <  8:133698/139‑1 (MQ=255)
                                                                                                       tACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCg                >  5:115107/1‑138 (MQ=255)
                                                                                                        aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt               <  5:26270/138‑1 (MQ=255)
                                                                                                        aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt               <  8:86625/138‑1 (MQ=255)
                                                                                                                   cAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtc   >  4:297611/1‑139 (MQ=255)
                                                                                                                    aaGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcg  <  6:121845/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                 
GGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCG  >  BS168_reference_genome/1474765‑1475019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑