Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,690,758 C→A 100% R214S (CGT→AGT)  codY → transcriptional regulator, GTP and BCAA‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,690,7580CA100.0% 137.0 / NA 39R214S (CGT→AGT) codYtranscriptional regulator, GTP and BCAA‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/25);  total (14/25)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAAT  >  BS168_reference_genome/1690623‑1690890
                                                                                                                                       |                                                                                                                                    
aaaGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGtt                                                                                                                                   <  7:46640/139‑1 (MQ=255)
aaaGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGtt                                                                                                                                   <  8:100195/139‑1 (MQ=255)
aaaGCTGTCGTACAAATGGCGATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGtt                                                                                                                                   <  6:96030/139‑1 (MQ=255)
  aGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCt                                                                                                                                 >  1:196685/1‑139 (MQ=255)
  aGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCt                                                                                                                                 >  5:97834/1‑139 (MQ=255)
                  gCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCa                                                                                                                  <  2:273217/138‑1 (MQ=255)
                     aTCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCa                                                                                                              <  1:237637/139‑1 (MQ=255)
                                 tCTTACAGGGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGCCCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    <  8:234204/137‑1 (MQ=255)
                                      cAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                              <  5:107368/138‑1 (MQ=255)
                                      cAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGT‑AAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg                                                                                            <  3:121142/139‑1 (MQ=255)
                                         tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt                                                                                          >  7:35969/1‑139 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                                        >  7:176486/1‑139 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt                                                                                          >  3:210952/1‑137 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt                                                                                          <  4:210952/137‑1 (MQ=255)
                                             cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGAAAAATTCCTGACCGTGTCGGCATTACCAGTACTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTa                                                                                      >  1:218916/1‑139 (MQ=255)
                                              ttGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt                                                                                     <  8:176486/139‑1 (MQ=255)
                                                gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGCAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTGCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt                                                                                     <  2:101911/137‑1 (MQ=255)
                                                gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                                    <  8:35969/138‑1 (MQ=255)
                                                      aTTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTc                                                                               >  4:234520/1‑137 (MQ=255)
                                                       ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGa                                                                                                            <  1:13600/107‑1 (MQ=255)
                                                       ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGa                                                                                                            >  2:13600/1‑107 (MQ=255)
                                                                  tttGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGa                                                                  <  6:97834/138‑1 (MQ=255)
                                                                      aggagCTTGACGGAAATGTAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  7:58209/139‑1 (MQ=255)
                                                                      aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACt                                                                                                                <  7:216379/88‑1 (MQ=255)
                                                                      aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACt                                                                                                                >  8:216379/1‑88 (MQ=255)
                                                                      aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  2:196685/139‑1 (MQ=255)
                                                                        gagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg                                                           <  7:181264/139‑1 (MQ=255)
                                                                        gagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg                                                           <  8:172901/139‑1 (MQ=255)
                                                                           cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             >  7:183070/1‑134 (MQ=255)
                                                                           cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  8:183070/134‑1 (MQ=255)
                                                                                    aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa                                               <  1:297676/139‑1 (MQ=255)
                                                                                             cttcttGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTa                                       >  6:250640/1‑138 (MQ=255)
                                                                                               tcttGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                                    <  7:241997/91‑1 (MQ=255)
                                                                                               tcttGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                                    >  8:241997/1‑91 (MQ=255)
                                                                                                      gCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    <  5:300367/68‑1 (MQ=255)
                                                                                                      gCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    >  6:300367/1‑68 (MQ=255)
                                                                                                         aGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTaaa                                     >  6:60313/1‑128 (MQ=255)
                                                                                                         aGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTaaa                                     <  5:60313/128‑1 (MQ=255)
                                                                                                                                 aTTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaaaat  <  2:218916/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
AAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAAT  >  BS168_reference_genome/1690623‑1690890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑