| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,271,523 | A→C | 100% | D45E (GAT→GAG) | uvrX ← | lesion bypass phage DNA polymerase; phage SPbeta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,271,523 | 0 | A | C | 100.0% | 150.7 / ‑6.6 | 48 | D45E (GAT→GAG) | uvrX | lesion bypass phage DNA polymerase; phage SPbeta |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (20/27); total (21/27) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATT > BS168_reference_genome/2271388‑2271658 | gatgaAAAGCTTCATTAGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTATCATTCCAAAAACTATATTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGACTAAcc > 8:59692/1‑139 (MQ=11) aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa < 8:39062/138‑1 (MQ=12) tCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTa > 5:51772/1‑138 (MQ=12) cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac < 2:109863/138‑1 (MQ=12) cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac < 4:258934/138‑1 (MQ=12) gtgGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAg < 6:300543/138‑1 (MQ=255) tACAATGTGTATCCTTGTTTCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATa < 5:114684/138‑1 (MQ=11) aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa < 1:231903/117‑1 (MQ=255) aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa > 2:231903/1‑117 (MQ=255) cattgtgTATCCTTGGATCTTCAGGTATCGCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCCGGAGATGCAGCTAATAATACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc < 7:59692/135‑1 (MQ=11) cAATTTGTATCCTTGTATCTTCAGGTATCTCAAATAGTCTCGTTCCTGTTTTGAATACAAAATCTTTTTTAAGTGCAGTAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc < 7:269703/138‑1 (MQ=11) cAATGTGTATCATTGGAGCTTCAGGTATCTCAAATAGTCGCGATCCTGTTTTGATTCCATAATCTTTTTTAAGTGATGCATATGAAGCTAATACTACACTTCCCTGTCTCTCCGTTATCCCTAAAACAGCAAGATAGc < 7:59694/138‑1 (MQ=11) tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGtt > 6:265108/1‑138 (MQ=255) tATCCTTGGTTCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa < 1:55605/138‑1 (MQ=255) tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCATGAGATGCAGCTAATAGTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa < 8:32765/138‑1 (MQ=11) tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaaa < 7:256324/139‑1 (MQ=255) tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa < 4:204325/138‑1 (MQ=255) tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa > 3:204325/1‑137 (MQ=255) tccTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAgg > 3:112207/3‑139 (MQ=255) ggATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGtt > 3:120045/1‑137 (MQ=255) ggATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGtt < 5:91924/137‑1 (MQ=255) ggATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGAATGTTAAAGGGtt > 1:184592/1‑137 (MQ=255) gATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAg < 7:141682/139‑1 (MQ=255) gATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAAAACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAg > 6:156571/1‑139 (MQ=255) tCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGcc > 8:22394/1‑139 (MQ=255) tCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCa > 5:179223/1‑139 (MQ=255) cAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCa < 7:22394/138‑1 (MQ=255) cAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTAAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAAGAGCAAGATAGCATGTTAATGGGTTAAGc < 8:224298/134‑1 (MQ=11) tctcAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTa > 6:142776/1‑138 (MQ=255) ctcAAATAGTCACCATCCCGCTTGGATACCAAAATCTATTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGCAATCCCTACAACAGCAAGATAGCATGCTAAAGGGTTAAGCCCCATTGTTACa < 5:276335/139‑1 (MQ=14) aaaTAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGc < 4:112207/138‑1 (MQ=255) cGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGaa < 4:120045/139‑1 (MQ=255) cGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGaa < 2:184592/139‑1 (MQ=255) gTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATaa > 4:93416/1‑138 (MQ=255) ttttGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATaa < 5:195570/137‑1 (MQ=255) aTTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATTCTACACTTCCATGTCTCTCCGTATTCCCTACAACAGC‑AGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGAtt > 4:159140/1‑138 (MQ=14) aTTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCATGTCTCTCCGTATTACCTACAACAGCAAGATAGCATGTTAAAGTGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGAtt > 7:248469/1‑139 (MQ=14) aaaaTCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCa > 5:125617/1‑73 (MQ=255) aaaaTCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCa < 6:125617/73‑1 (MQ=255) tttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCAGTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca < 6:51772/138‑1 (MQ=255) aGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGt < 5:156571/138‑1 (MQ=255) gCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTatat > 6:47772/1‑139 (MQ=255) cactTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGt > 6:209645/1‑138 (MQ=255) cactTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTa < 4:124381/139‑1 (MQ=255) cccTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCa > 7:59375/1‑58 (MQ=255) cccTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCa < 8:59375/58‑1 (MQ=255) cccTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTaatcaa > 8:164553/1‑139 (MQ=255) cccTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTaatcaa > 3:182289/1‑139 (MQ=255) tctctcCGTAATCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCAtt < 6:179223/139‑1 (MQ=255) | GATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATT > BS168_reference_genome/2271388‑2271658 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |