| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,839,474 | C→A | 29.2% | K133N (AAG→AAT) | yrbE ← | putative inositol‑related oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,839,474 | 0 | C | A | 29.2% | 54.1 / 3.2 | 38 | K133N (AAG→AAT) | yrbE | putative inositol‑related oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (8/15); new base A (7/2); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGATCGACATATCCATAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAGAGAGAAACTAACGGGCTTCTCACA > BS168_reference_genome/2839340‑2839590 | tGGATCGACATATCCATAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTa > 5:236190/1‑138 (MQ=255) aTCGACCTATCCATAAACAATCCGCCTGAAGTACGGACATAATCTATATTCGGTGGTTCTGGGTCCCTGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAAt < 5:129407/139‑1 (MQ=255) aCATATCCATAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGGTTTTTTATAAATTAGTGGTGTCCCGTTCACCATTTTGAAAAAA‑CGTTTTTATTTTTTTTATATGAg > 1:149056/1‑138 (MQ=255) aCATATCCATAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGGTTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTGTTTAAATTAgg > 5:104768/1‑139 (MQ=255) aCATATCCATAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGGGTTTTTATAAATTAGGTGTTCCGATTTGACCAATTTGAACAATAGGTTTTTTTTTTTTTTAAAtatg > 1:277636/1‑136 (MQ=255) tAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGCGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAgcg < 4:102912/139‑1 (MQ=255) tAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAgcg < 5:83455/139‑1 (MQ=255) aaCAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATTAGGTGTACCCATTTCACCAATTTAAACAAT‑CGTTTTGTTTTTCTTAAAATGAGGAGCAAAccgcg > 7:58070/1‑137 (MQ=255) aaTCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGt < 6:236190/139‑1 (MQ=255) tCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGTTTTTTAATAAATGAGGTGTACCGGTTTCACCATTTTTAACAAT‑CGTTTTGTTTTTTTTTAAATGTTGATAAAAGcggcgggt > 5:255999/1‑136 (MQ=255) tCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGTTTTTTAATAAAAGAGCTGTACCGAGTTGACCAATTAGAACAAT‑CCTGTTGAGTTTGATAAAAAGAGGATCAAAGCCGCCGtt > 8:172574/1‑138 (MQ=255) tCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTTATAAATGTGGTGTACCGATTTCACCATTTTCAACAAA‑CGTTTTGATTTTATTAAAATGAGGATCAAAGcggcgggt > 3:290722/1‑136 (MQ=255) tCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTAACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATAAAAGCGGCGGtt > 8:199387/1‑138 (MQ=255) tCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGAATTCACCAGTTTAAACAAT‑ATTTTTGATTTTATTTAAATTAGGATCAAAGagggggat > 4:217680/1‑136 (MQ=255) ccGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTGAACAAA‑CGTTTTGTTTTTATTTAAATTAGGATCAAAGCGGCGGGTa > 2:217997/1‑138 (MQ=255) gccgcaCGTCCGGACAAAATCTATTTTCGGGGGTGCGGGGTCCGGGGACGGTATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTAAAAATGAGGATCAACGCGGCGGTTAAAt < 2:277636/134‑1 (MQ=255) tACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTg < 1:217997/139‑1 (MQ=255) agagACAGAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTg < 3:35923/135‑1 (MQ=255) gggggTTCTGGGTCCCGGGACGTGGATTTTAATAAATTAGGTGTTCCGAATTCACCAGAATCAACAAA‑AGTTTTTGTTTTAGTAAAAAGTAGATAAAACGGGCGCTTAAATACGACTTGAAGAGTTTCCCCCTGTTTcc > 6:167022/1‑139 (MQ=255) ggTTCTGGGTCCCGGGACGTGTTTTTTAATAAATGAGGTGTTAAGATTTAACCATTTTAAACAAA‑CCTTTTTATTTTATTTAAATTTGGATTAAAGCGGCGGTTATATACGCCATGAATTGGTACACCGTTTTTCCggg > 6:247111/1‑138 (MQ=255) ggTTCTGGGGCCCGGGACGTGATTTTTAATAAAGGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGa < 6:255999/139‑1 (MQ=255) ttCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGAATTCACCATTTTCAACAAT‑CGTTTTGATTTTATTAAAATGATGATCAAAGCGGCGGGTAAATACGACATGAAGTGTTAACCCGTGTTTACGGaaa > 5:136735/1‑137 (MQ=255) ttCTGGGTCCCGGGACGTGATTTTTAAAAAATTAGGTGTACCGAATTAACCAATTTAATCAAT‑CGTATTTATTTTATTAAAATTTTGATCAAAGCCGCGGTTTAATCCGAAATGAAGTGTTACCCCGGGTTTCCGGAcc > 2:189780/1‑138 (MQ=255) gTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCa < 3:94439/139‑1 (MQ=255) tCCCGGGACGTTATTTTTAATAAATGAGGTGTACCTATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAATTTCGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCaa < 5:167022/139‑1 (MQ=255) ccGGTACGTTATTTTTAATAATTGAGGTGTACCGATTTCACCATTTTAAAAAAT‑CGTTTTTATTTTATTAAAATGATGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAg < 2:154648/138‑1 (MQ=255) ggACGTGTTTTTTAATAAATGAGGTGTTCCGGTTTCACCATTTTGAACAAT‑CGTTTTGATTTTGTTAAAATGAGGATCAAAGCGGCGGGTTAATACGACTTGAAGTGTTACCCCGTGTTTGCGGACCGACGCCAgcgcg > 4:94439/1‑138 (MQ=255) gTGATTTTTAATAACTGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCAc < 3:217680/139‑1 (MQ=255) tGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTAAACAAT‑CGTTTTTATTTTCTTAAAATGAGGATCAAATCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCAGGACCGCCGCAACCCCTTCACt > 6:295258/1‑139 (MQ=255) tGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCagag < 4:174969/139‑1 (MQ=255) ggttcaCCGATTTCACCATTTTAACAAAT‑CTTTTTTATTTTATTAAAATGAGTATCAACGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAgagaga < 5:35825/134‑1 (MQ=255) ttCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAGAGAGAAACTAACGGGc > 3:157538/1‑139 (MQ=255) aCCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAGAGAGAAACTAACGGGCTtc > 5:109419/1‑139 (MQ=255) ttttCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAGAGAGAAACTAACGGGCTTCTcac < 5:117822/139‑1 (MQ=255) tttCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAGAGAGAAACTAACGGGCTTCTcac > 5:172466/1‑138 (MQ=255) ttCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTATCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAGAGAGAAACTAACGGGCTTCTcaca < 1:189780/138‑1 (MQ=255) t‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCg > 3:112912/1‑65 (MQ=255) t‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCg < 4:112912/65‑1 (MQ=255) | TGGATCGACATATCCATAAACAATCCGCCTGACGTACGGACATAATCTATATTCGGGGGTTCTGGGTCCCGGGACGTGATTTTTAATAAATGAGGTGTACCGATTTCACCATTTTCAACAAT‑CGTTTTGATTTTCTTAAAATGAGGATCAAAGCGGCGGTTAAATCCGACTTGAAGTGTTACCCCGTGTTTCCGGACAGCCGCAAGCGCTTCACTCGTTTCGTCCAGAGAGAAACTAACGGGCTTCTCACA > BS168_reference_genome/2839340‑2839590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |