| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 3,021,860 | C→A | 8.2% | L210M (CTG→ATG) | ppnKB → | inorganic polyphosphate/ATP‑NAD kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 3,021,860 | 0 | C | A | 8.2% | 234.7 / 8.8 | 73 | L210M (CTG→ATG) | ppnKB | inorganic polyphosphate/ATP‑NAD kinase |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base A (3/3); total (33/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
CCTATAACAAGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCAAAACAGTCAAACTAAAG > BS168_reference_genome/3021726‑3021994 | ccTATAACAAGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGAc < 8:127017/139‑1 (MQ=255) ataACAACTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACAGAAAGCTGACCCt > 2:62026/1‑139 (MQ=255) aaCAAGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTc > 4:235231/1‑139 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCGATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTTCCGGACACTCGGCTCGGCATTTGTCCTCAGCTCTGACCGAAAGCTGACCATTCgtg < 1:276006/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 5:39522/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 3:235231/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 3:31082/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 6:257710/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 7:240822/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 7:32580/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 7:85876/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 8:232272/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGACCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 4:275404/139‑1 (MQ=255) aaGTCTGTTGCAGGAGCTGGTGCTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtg < 2:266776/139‑1 (MQ=255) aGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgtgt > 1:106506/1‑139 (MQ=255) gTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGCCCGAAAGCTGACCCTTCGtgtg > 3:31112/1‑139 (MQ=255) gTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGtgtg > 8:97013/1‑139 (MQ=255) gTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGtgtg > 4:261012/1‑139 (MQ=255) gTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGtgtg > 3:212772/1‑139 (MQ=255) gTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGtgtg > 3:131899/1‑139 (MQ=255) gCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAg < 6:207736/138‑1 (MQ=255) gCTGTTGTTGATCCATTGCTTCCATTCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTAAAGGACACTCGGCTAGCCATATTTCCTCAGCTCTGACCGAAAGCAGACCCTTCGTGTGGTGAAGGACGGc > 8:51152/1‑138 (MQ=255) gttgttGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGGAGGACGGCAACg < 1:62026/139‑1 (MQ=255) ttgttgATCCATTGCTTCCATGCATGCAGGTATCAGAGCTAGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACg < 5:170666/138‑1 (MQ=255) tgttgATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGaa > 1:227062/1‑139 (MQ=255) aTTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAat > 5:73092/1‑138 (MQ=255) aTTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAat > 8:89525/1‑138 (MQ=255) ttGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAatca < 2:106506/139‑1 (MQ=255) ccATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAatca < 1:186982/133‑1 (MQ=255) ccATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAatca > 2:186982/1‑133 (MQ=255) ccATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGc < 3:190876/138‑1 (MQ=255) ccATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCt > 5:89917/1‑139 (MQ=255) ccATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGGGTGGTGCAGGACGGCAACGAACACCCAATCATCGGc < 4:241117/138‑1 (MQ=255) agagCTTGCATCCCTTAACAATACTACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACt < 6:240163/138‑1 (MQ=255) agagCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTc < 1:5342/139‑1 (MQ=255) gCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGCCCCTTCGTGTGGTGCAGGCCGGCAACGCACACCCAATCATCGGCTTGGACAATGACGCACTCAGCAc > 4:89149/1‑137 (MQ=255) gCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGAc > 2:106583/1‑120 (MQ=255) gCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGAc < 1:106583/120‑1 (MQ=255) gCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCAc > 6:136210/1‑137 (MQ=255) aCAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGc < 8:157817/104‑1 (MQ=255) aCAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGc > 7:157817/1‑104 (MQ=255) ataCCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGAt < 3:261012/139‑1 (MQ=255) ataCCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGAt < 7:97013/139‑1 (MQ=255) ataCCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGAt > 7:151099/1‑139 (MQ=255) ataCCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGAt < 2:227652/139‑1 (MQ=255) ataCCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTGGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGAt > 4:81681/1‑139 (MQ=255) ataCCTACAGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGAt < 4:23251/139‑1 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGCCCCTTCGTGTGGTGCAGGACGGCACCGAACCCCCAAGCATCGGCTTGGACAATGAAGCACACAGCACGAGAAATGTGAAAACGATCGaaa > 6:258360/1‑138 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgt > 8:124701/1‑56 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgt < 7:124701/56‑1 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgt < 7:128600/56‑1 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCgt > 8:128600/1‑56 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGc > 8:32749/1‑71 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGc < 7:32749/71‑1 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGa > 7:294116/1‑97 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGa < 8:294116/97‑1 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAAt > 2:33909/1‑139 (MQ=255) accGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTGGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAAt > 2:83193/1‑139 (MQ=255) cacTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGCCCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACt > 4:219396/1‑139 (MQ=255) tCGGATCGCAATTTGTCCTAAGATCTGCCCGAAAGCTGACCCGTAGTTTGGTGCAGGACGGCAAAGAACACCCAATCATCGGCTTGGACAAAGAAGCACTCAGAAAGAGAAATGTGAAAACGATCGAAATCAAACttt > 8:277690/1‑136 (MQ=255) ggCTCGCCATTTGTCCTCAGCTCTGACCGAAAGATGAc < 7:116726/38‑1 (MQ=255) ggCTCGCCATTTGTCCTCAGCTCTGACCGAAAGATGAc > 8:120611/1‑38 (MQ=255) ggCTCGCCATTTGTCCTCAGCTCTGACCGAAAGATGAc > 8:116726/1‑38 (MQ=255) ggCTCGCCATTTGTCCTCAGCTCTGACCGAAAGATGAc < 7:120611/38‑1 (MQ=255) ggCTCGCCATTTGTCCTCAGCTCTGACAGAAAGATGAc > 6:33948/1‑38 (MQ=255) ggCTCGCCATTTGTCCTCAGCTCTGACAGAAAGATGAc < 5:33948/38‑1 (MQ=255) cTCAGCTCTGACCGTAAGCTTACCCTTAGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTTGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCaaaa < 7:51152/139‑1 (MQ=255) cTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCaaaa < 8:151099/139‑1 (MQ=255) gCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAACGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCAAAACAGt < 4:131899/139‑1 (MQ=255) tGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCAAAACAGTCa < 5:136210/137‑1 (MQ=255) tGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCAAAACAGTCa < 7:170360/137‑1 (MQ=255) ccGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCAAAACAGTCAAACTa < 4:212772/139‑1 (MQ=255) aaGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCAAAACAGTCAAACTAAAg > 3:127062/1‑138 (MQ=255) | CCTATAACAAGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCTTGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTGTGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAAATCAAACTGTCTAATAAGAAAATCAAAACAGTCAAACTAAAG > BS168_reference_genome/3021726‑3021994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |