Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 3,036,709 C→T 6.3% R388H (CGC→CAC)  tyrS ← tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome3,036,7090CT 6.3% 202.8 / 2.2 62R388H (CGC→CAC) tyrStyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (23/35);  new base T (2/2);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGCCTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGTCAACTTCCATAGAAGGAAC  >  BS168_reference_genome/3036573‑3036842
                                                                                                                                        |                                                                                                                                     
cTTTGCCTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCg                                                                                                                                      <  2:99445/138‑1 (MQ=255)
   tGCCTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCTGTCTGGCGtt                                                                                                                                    <  2:160283/137‑1 (MQ=255)
   tGCCTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGtt                                                                                                                                    <  3:270575/137‑1 (MQ=255)
   tGCCTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGtt                                                                                                                                    <  6:266998/137‑1 (MQ=255)
      cTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACTAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCg                                                                                                                               <  1:184573/139‑1 (MQ=255)
      cTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCg                                                                                                                               <  4:254486/139‑1 (MQ=255)
      cTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCg                                                                                                                               <  2:296193/139‑1 (MQ=255)
      cTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCg                                                                                                                               <  3:286776/139‑1 (MQ=255)
      cTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCg                                                                                                                               <  7:58554/139‑1 (MQ=255)
          aGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTa                                                                                                                            <  7:84753/138‑1 (MQ=255)
                aaaGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTaaa                                                                                                                     <  2:202751/139‑1 (MQ=255)
                aaaGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTaaa                                                                                                                     <  1:242591/139‑1 (MQ=255)
                 aaGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTaaa                                                                                                                     >  7:20160/1‑138 (MQ=255)
                          tttCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTc                                                                                                               <  2:166283/135‑1 (MQ=255)
                                atttatACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGTGTTCACCGTTAATGTAAACAGCTCCGTTTTGGAt                                                                                                     >  1:81985/1‑139 (MQ=255)
                                atttatACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGTGTTCACCGTTAATGTAAACAGCTCCGTTTTGGAt                                                                                                     >  1:77590/1‑139 (MQ=255)
                                atttatACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTgg                                                                                                       <  4:248026/137‑1 (MQ=255)
                                atttatACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGAt                                                                                                     >  3:282762/1‑139 (MQ=255)
                                  ttatACGTAACAAGGAAGTATTTTTTCTTACCGCGGCGCAGTACAGTAAATTGGTTTTCTATGTGGTCTTCACCTGTTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATg                                                                                                    <  2:292842/138‑1 (MQ=255)
                                          cacaAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGtt                                                                                                                                    >  8:115334/1‑98 (MQ=255)
                                          cacaAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGtt                                                                                                                                    <  7:115334/98‑1 (MQ=255)
                                          cacaAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTTGATGTCTTAAccc                                                                                           >  6:38922/1‑137 (MQ=255)
                                          cacaAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCAcgc                                                                                           >  6:270187/1‑139 (MQ=255)
                                          cacaAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCAGTCTGGCGTTCACCGATAATGTAAACAGCTCCGTTTTGGATGTCTTCAcgc                                                                                           >  6:156552/1‑139 (MQ=255)
                                             aaGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGTGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCt                                                                                        <  2:81985/139‑1 (MQ=255)
                                             aaGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGTGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCt                                                                                        <  2:77590/139‑1 (MQ=255)
                                                          ttcttcCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGaa                                                                            <  5:193932/138‑1 (MQ=255)
                                                               cccGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGgag                                                                        <  6:83378/137‑1 (MQ=255)
                                                                            aCAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCa                                                         <  3:134840/139‑1 (MQ=255)
                                                                                tAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCaa                                                     >  5:21650/1‑139 (MQ=255)
                                                                                tAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCaa                                                     >  5:81223/1‑139 (MQ=255)
                                                                                tAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCaa                                                     >  1:170385/1‑139 (MQ=255)
                                                                                  aaTTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAac                                                    <  2:177580/138‑1 (MQ=255)
                                                                                  aaTTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGGTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCAAGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAac                                                    <  5:156552/138‑1 (MQ=255)
                                                                                        ttttCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCt                                              >  2:71885/1‑138 (MQ=255)
                                                                                          ttCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCt                                                                                                                <  7:226208/70‑1 (MQ=255)
                                                                                          ttCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCt                                                                                                                >  8:226208/1‑70 (MQ=255)
                                                                                               tGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGa                                      <  4:282762/139‑1 (MQ=255)
                                                                                                 cGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGaa                                     <  7:26719/138‑1 (MQ=255)
                                                                                                 cGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGGTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGaa                                     <  2:23849/138‑1 (MQ=255)
                                                                                                  ggTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAg                                   <  1:71885/139‑1 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAGACAGCGCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCACCACATCTACAAGTGAAAGCTCTGGGGTGCt                       >  4:240288/1‑139 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTg                                                                                      >  1:151703/1‑76 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTg                                                                                      <  2:151703/76‑1 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGa                                                                             <  8:96058/85‑1 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGa                                                                             >  7:96058/1‑85 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAac                                                    <  6:175859/110‑1 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAac                                                    >  5:175859/1‑110 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCt                       >  7:90548/1‑139 (MQ=255)
                                                                                                              aTAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCt                       >  2:10048/1‑139 (MQ=255)
                                                                                                                aGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGt                     >  7:175513/1‑139 (MQ=255)
                                                                                                                  ggTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGAt                                                                      <  5:187046/88‑1 (MQ=255)
                                                                                                                  ggTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGAt                                                                      >  6:187046/1‑88 (MQ=255)
                                                                                                                    tataATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGTCAAc                 >  5:220027/1‑139 (MQ=255)
                                                                                                                     ataATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGTCAACt                >  8:165564/1‑139 (MQ=255)
                                                                                                                            atttCCGTCTGGCGTTCACCGTTAATGTAACCAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCATCACATCTACAAGTGAAAGCTCGTGGGTGCTGTCAACTTCCa            >  6:85776/1‑136 (MQ=255)
                                                                                                                                  gTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGTCAACTTCCATAGAAGGaa   <  7:299091/139‑1 (MQ=255)
                                                                                                                                  gTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGGCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGTCAACTTCCATAGAAGGaa   <  8:20160/139‑1 (MQ=255)
                                                                                                                                   tCTGGCGTTCCCCGTTAATGTAAACAGCTCCGTTTTGGCTGGCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGTCAACTTCCATAGAAGGAAc  <  2:170385/139‑1 (MQ=255)
                                                                                                                                   tCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGAGAAAGCTCTTGGGTGCTGTCAACTTCCATAGAAGGAAc  <  4:196418/139‑1 (MQ=255)
                                                                                                                                       gCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTg                                                                                      <  2:57943/51‑1 (MQ=255)
                                                                                                                                       gCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTg                                                                                      >  1:57943/1‑51 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CTTTGCCTTCAGTGGCAAAGGATCTTTTTCTTATTTATACGTCACAAGGAAGTATTTTTTCTTCCCGCGGCGCAGAACAGTAAATTGGTTTTCGATGCGGTCTTCACCTGATAGGGTATAATTTATTTCCGTCTGGCGTTCACCGTTAATGTAAACAGCTCCGTTTTGGATGTCTTCACGCGCTTGGCGTTTAGAAGGAGATAATTTAGATTGCACCAACACATCTACAAGTGAAAGCTCTTGGGTGCTGTCAACTTCCATAGAAGGAAC  >  BS168_reference_genome/3036573‑3036842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑