Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	3,141,615	A→G	6.4%	A270A (GCA→GCG)	ythB →	putative cytochrome bd menaquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,141,615	0	A	G	6.4%	160.6 / 2.8	63	A270A (GCA→GCG)	ythB	putative cytochrome bd menaquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base G (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AGCAAACTGGCTGTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCA  >  BS168_reference_genome/3141477‑3141750                                                                                                                                            |                                                                                                                                        aGCAAACTGGCTGTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCa                                                                                                                                         >  1:206759/1‑139 (MQ=255)      aCTGGCTGTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACttgt                                                                                                                                    >  4:295781/1‑139 (MQ=255)          gCTGTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGc                                                                                                                                 >  5:145582/1‑138 (MQ=255)           cTGTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGa                                                                                                                               <  3:295781/139‑1 (MQ=255)            tGTACAGCGGAATGATGAATGATTTTTCTTGGATTATAGCATAGTTCAGTACATTTGTCATTGCTGGAATCGCTCTTTATCTGCCGAAAAAAATCTTTGGTCAAAACAT‑CGGAAAACCGCGTCGCGAACTTGGTGGGAt                                                                                                                              >  5:42255/1‑139 (MQ=255)             gTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGAtt                                                                                                                             >  8:52006/1‑139 (MQ=255)             gTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGAtt                                                                                                                             >  7:225559/1‑139 (MQ=255)             gTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGAtt                                                                                                                             >  8:177329/1‑139 (MQ=255)             gTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGAtt                                                                                                                             >  1:34256/1‑139 (MQ=255)              tACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTg                                                                                                                            <  2:34256/139‑1 (MQ=255)              tACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTg                                                                                                                            <  8:225559/139‑1 (MQ=255)                  gCGGAATGATGATTGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAAGCGCTCTTTTTCTTCAAAACAATAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTTTTTAGATTgggtt                                                                                                                        >  1:211796/1‑137 (MQ=255)                  gCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCACGCACTTGTTGCGATTGGGa                                                                                                                         >  8:270074/1‑138 (MQ=255)                   cGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGAt                                                                                                                        >  5:56962/1‑138 (MQ=255)                   cGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATa                                                                                                                       <  7:3692/139‑1 (MQ=255)                   cGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATa                                                                                                                       <  4:148391/139‑1 (MQ=255)                   cGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATa                                                                                                                       >  1:201189/1‑139 (MQ=255)                        tgatgaatgatTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑GGGTAAACCGCGTCTCGCACTTGTTGCGAGTGGGTTAGAGtg                                                                                                                  >  4:274194/1‑138 (MQ=255)                        tgatgaatgatTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTa                                                                                                                  >  3:109156/1‑139 (MQ=255)                             aatgatTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTtc                                                                                                             >  5:269385/1‑139 (MQ=255)                                          tttttattGCAACGTTCATTTCATTTGTTATTTGTGGGATAGCTGTTTTTCTGCCTAAGAAAAGCTTTGGTAAAAAAATCCGTAAAGCCGCGTCTCTCACTTGTTGCGATTGGGATTCAGTATTTTCTCGCAAGCTAc                                                                                                  <  2:211796/135‑1 (MQ=255)                                                   cATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTAcgggcg                                                                                       <  6:254541/139‑1 (MQ=255)                                                     tCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTAcgggcggg                                                                                     >  6:1597/1‑139 (MQ=255)                                                     tCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTAcgggcggg                                                                                     <  7:204175/139‑1 (MQ=255)                                                     tCGTTCATTACATTTGTCATTGCTGGAATCGCGCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCCCTTGTTGCGATTGGGATACAGTATTTTCTCGGAAGCTCCGCGTAcgggcggg                                                                                     >  4:212402/1‑139 (MQ=255)                                                        ttCATTACATTTGTCATTGCTGGAATCGCTGTTTTTCTGCCTAACAAAAGCTTTGGTAAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGTTACAGTATTTTCTCGCAAGATACGCGTACGGGCGGgcgc                                                                                  >  1:252020/1‑139 (MQ=255)                                                          cATTACATTTGTCATTGCTGGAATCGCTCTTTATCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCAt                                                                                >  3:249448/1‑139 (MQ=255)                                                              aCATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             <  7:9873/138‑1 (MQ=255)                                                              aCATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             <  3:256512/138‑1 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTGTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAA‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCGTGCGta                                                                        >  5:190237/1‑139 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCGCTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             <  4:194632/134‑1 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCGCTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             <  4:190668/134‑1 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCGCTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             >  3:190668/1‑134 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCGCTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             >  3:194632/1‑134 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGta                                                                        >  7:144674/1‑139 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGta                                                                        >  7:49194/1‑139 (MQ=255)                                                                  ttGTCATTGCTGGAATCGCTCTTTTTATGCCTTACAAAAGCGTTGGTGAAAACAT‑TGGAAAACCGGGTCTCGGACATGTTGCGATTGGGATACAGTATTTTCTCGCAATCTACGCGAACGGGCGGGGGCGTCGTGCGtt                                                                        >  2:99278/1‑138 (MQ=255)                                                                         tGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGAtt                                                                 >  3:105318/1‑139 (MQ=255)                                                                          gCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTGAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGCTTGGGATACAGTATTTTATCGCAAGCTACGCGTACGGGCGGGCGCCTCTTCCGTATAGTAttt                                                                >  8:14167/1‑139 (MQ=255)                                                                          gCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGAttt                                                                >  8:162667/1‑139 (MQ=255)                                                                                     tctTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGAtgt                                                      >  2:234318/1‑138 (MQ=255)                                                                                         tttCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTACGTATATGATTTATCCCGATGTGa                                                    <  7:162667/136‑1 (MQ=255)                                                                                          ttCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAg                                                 <  6:145582/138‑1 (MQ=255)                                                                                          ttCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAg                                                 <  6:56962/138‑1 (MQ=255)                                                                                                 tAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGt                                           >  2:274411/1‑137 (MQ=255)                                                                                                   aCAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAgg                                       <  7:90999/139‑1 (MQ=255)                                                                                                   aCAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAgg                                       >  4:151365/1‑139 (MQ=255)                                                                                                         gCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTcac                                 <  6:39711/139‑1 (MQ=255)                                                                                                               gTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGc                            <  1:14362/138‑1 (MQ=255)                                                                                                                   aaaCAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCa                       <  4:249448/139‑1 (MQ=255)                                                                                                                     aCAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAAc                     >  3:257026/1‑139 (MQ=255)                                                                                                                        t‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGtt                  <  6:42255/139‑1 (MQ=255)                                                                                                                           ggAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCa                <  7:270074/139‑1 (MQ=255)                                                                                                                                 ccGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAgcgc          <  8:32088/139‑1 (MQ=255)                                                                                                                                  cgcgTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTAAGAGCGCt         >  5:173532/1‑139 (MQ=255)                                                                                                                                      tctcGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             >  7:169598/1‑67 (MQ=255)                                                                                                                                      tctcGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCtt                                                                             <  8:169598/67‑1 (MQ=255)                                                                                                                                       ctcGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTg    <  7:281139/139‑1 (MQ=255)                                                                                                                                          gCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCa  <  4:105318/138‑1 (MQ=255)                                                                                                                                          gCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCa  <  8:144674/138‑1 (MQ=255)                                                                                                                                          gCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCa  <  3:274194/138‑1 (MQ=255)                                                                                                                                          gCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCa  <  1:8388/138‑1 (MQ=255)                                                                                                                                          gCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCa  <  1:234318/138‑1 (MQ=255)                                                                                                                                          gCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCa  <  8:49194/138‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        AGCAAACTGGCTGTACAGCGGAATGATGAATGATTTTTCTTGGATTATTGCATCGTTCATTACATTTGTCATTGCTGGAATCGCTCTTTTTCTGCCTAACAAAAGCTTTGGTCAAAACAT‑CGGAAAACCGCGTCTCGCACTTGTTGCGATTGGGATACAGTATTTTCTCGCAAGCTACGCGTACGGGCGGGCGCATCTTCCGTATATGATTTATCCCGATGTGACAGTCATGTCAGGCTTCACAGAGCCAGCAACGTTCAGAGCGCTGTTTGCA  >  BS168_reference_genome/3141477‑3141750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑