Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 1,317,152 +GT intergenic (+23/+5) phrA → / ← yjpA secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,317,1521.G95.6% 332.9 / ‑2.3 93intergenic (+23/+5)phrA/yjpAsecreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand):  ref base . (2/0);  major base G (54/33);  minor base T (3/1);  total (59/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*BS168_reference_genome1,317,1522.T93.5% 304.0 / 12.6 93intergenic (+23/+5)phrA/yjpAsecreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand):  ref base . (5/1);  new base T (54/33);  total (59/34)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑G‑‑C‑TTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCT  >  BS168_reference_genome/1317020‑1317274
                                                                                                                                         ||                                                                                                                            
tgttgCTCGTTGCGGTCGGGTTCAGCTTTTCTCAGGTGATGGGTCATGCAGGTGCAACAGCAAACACAGAAGGGAAAACATTTCAAATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑G‑‑G‑tcttt                                                                                                                       >  1:503141/1‑139 (MQ=255)
tgttgCTCGTTGCGGTCGGGTTCAGCTTTACGCAGGGGATGGTTCATGCAGGTGCAACAGCCAACACAGAAGGGAAAACATTTCATATTTCGGGACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑g‑g‑gggcttt                                                                                                                          >  4:44023/1‑133 (MQ=255)
       cGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑G‑‑G‑TCttt‑tttattt                                                                                                               >  6:361560/1‑135 (MQ=255)
            cGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCa                                                                                                             <  1:254532/139‑1 (MQ=255)
              gTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGc                                                                                                           <  3:289563/139‑1 (MQ=255)
                 gggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTc                                                                                                        <  4:179443/139‑1 (MQ=255)
                   gTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAGAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCa                                                                                                      <  5:77691/139‑1 (MQ=255)
                      cAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCAtt                                                                                                    <  6:271564/138‑1 (MQ=255)
                            tACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTa                                                                                              >  5:132183/1‑138 (MQ=255)
                             aCTCAGGTGATGGTTCATGCAGGTGAAACGGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATc                                                                                            >  2:472086/1‑139 (MQ=255)
                             aCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATc                                                                                            >  6:493849/1‑139 (MQ=255)
                                 ctgTGATGGTTCATGCAGGTGAAACAGCAAACACAGAACGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  6:294552/135‑1 (MQ=255)
                                 aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  6:313692/138‑1 (MQ=255)
                                 aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          >  3:357178/1‑137 (MQ=255)
                                 aGGTGATGGCTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  6:439387/137‑1 (MQ=255)
                                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGTCACGCAATCAAACAACATGCAT‑AAAAAAAGACCCATA‑GTG‑GT‑CTTTTTT‑ATTTATTCCGCGTCCAAGATTTTATAGTAAGc                                                                                      >  1:191561/1‑139 (MQ=255)
                                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                      >  2:1933/1‑139 (MQ=255)
                                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                      >  3:423920/1‑139 (MQ=255)
                                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                      >  7:152393/1‑139 (MQ=255)
                                      aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCt                                                                                     >  3:184381/1‑137 (MQ=255)
                                           tCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCag                                                                                  >  7:132226/1‑135 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          >  5:494783/1‑125 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  6:494783/125‑1 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCagaagagg                                                                            >  4:61688/1‑137 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            >  7:18606/1‑139 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            >  5:250000/1‑139 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            >  6:348279/1‑139 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            >  3:413820/1‑139 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            >  8:333847/1‑139 (MQ=255)
                                             aTGCAGGTGAAACAGCAAACACAGAACGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            <  2:301196/139‑1 (MQ=255)
                                                cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCAc                                                                         <  8:132226/139‑1 (MQ=255)
                                                cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCAc                                                                         <  6:168388/139‑1 (MQ=255)
                                                   gTGACACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAAGCACACAGGATGCAT‑AAAAAAAGACCCTTA‑GTG‑GT‑CGTTTTT‑ATTTCTTCAGCTTCCATTCGTTTATAGTCAGCTCAGAAGATCCCCtgg                                                                      >  3:502863/1‑137 (MQ=255)
                                                   gTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTg                                                                      >  4:194286/1‑139 (MQ=255)
                                                   gTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTg                                                                      >  3:265012/1‑139 (MQ=255)
                                                   gTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTAA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCCGCTCAGAAGATCCACATg                                                                      >  8:339982/1‑139 (MQ=255)
                                                    tGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCa                                                                                                      >  8:360731/1‑106 (MQ=255)
                                                    tGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCa                                                                                                      <  7:360731/106‑1 (MQ=255)
                                                             aaaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCg                                                             >  4:126802/1‑138 (MQ=255)
                                                                   aGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          >  7:284874/1‑103 (MQ=255)
                                                                   aGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  8:284874/103‑1 (MQ=255)
                                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                     >  3:476963/1‑139 (MQ=255)
                                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                     >  6:102717/1‑139 (MQ=255)
                                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                     <  5:493849/139‑1 (MQ=255)
                                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                     <  2:262159/139‑1 (MQ=255)
                                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATAGTCAGCTCAGAATATCCACTTGCCCCCCCCGGATCCGCa                                                     >  3:285203/1‑139 (MQ=255)
                                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTAAAGCTTACATTCTTTTATAGTCAGCTCAGAAGATACACTTTCCACCAGCGGATCCg                                                       >  3:26400/1‑137 (MQ=255)
                                                                      aGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTGCATTCTTTTATCGTCAGCTCAGAAGCTCCACTTGCCACCCGCGGATCCGCATg                                                   >  2:214946/1‑139 (MQ=255)
                                                                      aGGCAAAACATTTCAAATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑GCGT‑C‑TTTTTAAATTCTTCAGATTCCATTCTTTTATCGTAAGCTCATAACATCCAATTGCCACCACCCGAACCGCATg                                                   >  8:221442/1‑139 (MQ=255)
                                                                       gggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATgg                                                  <  1:173423/139‑1 (MQ=255)
                                                                        ggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGc                                                 <  4:265012/139‑1 (MQ=255)
                                                                          aaaaCATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGcc                                                >  3:80240/1‑138 (MQ=255)
                                                                          aaaaCATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCGTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGCTCCGCATGGCCg                                               >  2:137661/1‑139 (MQ=255)
                                                                            aaCATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                    <  1:404608/100‑1 (MQ=255)
                                                                            aaCATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                    >  2:404608/1‑100 (MQ=255)
                                                                            aaCATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGAt                                             <  3:126802/139‑1 (MQ=255)
                                                                             aCATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGAtt                                            >  6:182632/1‑139 (MQ=255)
                                                                                 ttCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGCTTTCCg                                        >  1:308077/1‑139 (MQ=255)
                                                                                 ttCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                        >  5:488895/1‑139 (MQ=255)
                                                                                 ttCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                        >  6:182875/1‑139 (MQ=255)
                                                                                 ttCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCGTTTTT‑ATTTCTTCAGGTTCCATTCTTTTATCGGCAGCTAAGAAGATCCACTTGCCCCCAGCGGATCCGGATGGCCGAGTTGCg                                        >  3:324891/1‑139 (MQ=255)
                                                                                 ttCATATTGCGGCACGCAATCAAACATGACGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                        >  5:103381/1‑139 (MQ=255)
                                                                                  tCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                    <  5:466483/94‑1 (MQ=255)
                                                                                  tCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                    >  6:466483/1‑94 (MQ=255)
                                                                                  tCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                        <  5:361560/138‑1 (MQ=255)
                                                                                  tCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                        <  1:19958/138‑1 (MQ=255)
                                                                                  tCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTATTCAGCTTCCATTCTTTTATCGTCAGATCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGc                                       >  4:451885/1‑139 (MQ=255)
                                                                                   cATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGAGCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCACAGAAGATACACATGCCACCCGCGGATCCGCATGGCCGCTTTCCGcg                                      >  2:221324/1‑138 (MQ=255)
                                                                                    atatTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTTTCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTg                                     >  7:180893/1‑139 (MQ=255)
                                                                                     tatTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc                                    <  3:214316/139‑1 (MQ=255)
                                                                                        tGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGccgc                                 >  1:203696/1‑137 (MQ=255)
                                                                                        tGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctc                                 >  1:326702/1‑139 (MQ=255)
                                                                                        tGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctc                                 >  8:84626/1‑139 (MQ=255)
                                                                                         gCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGCTCCACTTGCCCCCAGCGGATCCGCATGGCCGATTTCCGCTGCctct                                >  3:293112/1‑139 (MQ=255)
                                                                                          gggCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctct                                <  7:257158/137‑1 (MQ=255)
                                                                                          cGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctct                                <  2:159672/138‑1 (MQ=255)
                                                                                                       aaCATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGAATTCCGCTGCCTCTTCCAGTGAATCTga                  >  4:89544/1‑138 (MQ=255)
                                                                                                         cATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCtt                <  4:431935/139‑1 (MQ=255)
                                                                                                           tgatgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCAAGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg              <  8:194832/139‑1 (MQ=255)
                                                                                                            gatgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa             >  3:388405/1‑139 (MQ=255)
                                                                                                            gatgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa             >  1:67767/1‑139 (MQ=255)
                                                                                                            gatgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATAGTCAGCTCAGAAGAACCACTTGCCACCAGCGGCTCCGCATGGCCGATTTCCGCTGCCAATTCGAGTGAATCTGATTCGa             >  2:436841/1‑139 (MQ=255)
                                                                                                             atgCAT‑AAAAACCGCCCCTTACG‑G‑GT‑CTTTTTT‑ATTTCTTCAGCTACCATTCTTTTATCCTCAGCTCAGAACAGCCACTTGCCCCCAGCGGATCCGCATGGCCGAATTCCTCTGCCTCTTCCAGTGAATCGGCTTCGAt            <  8:473013/139‑1 (MQ=255)
                                                                                                             atgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt            <  4:388405/139‑1 (MQ=255)
                                                                                                              tgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTTTCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGCTCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATg           >  5:349186/1‑139 (MQ=255)
                                                                                                              tgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGCTCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCGGCCTCTTCCAGTGAATCTGCTTCGAt            >  2:114904/1‑138 (MQ=255)
                                                                                                              tgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                      <  6:420519/112‑1 (MQ=255)
                                                                                                              tgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                      >  5:420519/1‑112 (MQ=255)
                                                                                                              tgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt            >  8:290491/1‑138 (MQ=255)
                                                                                                              tgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt            <  4:80240/138‑1 (MQ=255)
                                                                                                              tgCAT‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATg           >  3:324799/1‑139 (MQ=255)
                                                                                                                  t‑AAAAAAAGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCCGCGGATCCGCCTGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa        >  1:88927/1‑138 (MQ=255)
                                                                                                                       aaaaGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGc   <  8:18606/139‑1 (MQ=255)
                                                                                                                        aaaGACCCTTA‑G‑G‑GGTCTTTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCt  <  5:182875/139‑1 (MQ=255)
                                                                                                                                         ||                                                                                                                            
TGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAAGACCCTTA‑G‑G‑G‑‑C‑TTTTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCT  >  BS168_reference_genome/1317020‑1317274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑