BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	4,155,390:1	+A	100%	intergenic (‑181/+43)	trnY‑Lys ← / ← purA	tRNA‑Lys/adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	4,155,390	1	.	A	97.8%	310.1 / ‑0.3	89	intergenic (‑181/+43)	trnY‑Lys/purA	tRNA‑Lys/adenylosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (34/53); total (34/55)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 104 total aligned reads displayed.

GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAAC‑AAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCT  >  BS168_reference_genome/4155253‑4155521

gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaac                                                                                                                                      >  1:10627/1‑138 (MQ=255)

gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaac                                                                                                                                      >  7:291605/1‑138 (MQ=255)

gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaac                                                                                                                                      >  7:208642/1‑138 (MQ=255)

gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaac                                                                                                                                      >  3:54762/1‑138 (MQ=255)

      aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaa                                                                                                                               >  7:291078/1‑137 (MQ=255)

          tGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGc                                                                                                                           >  8:12810/1‑139 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAAGTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaa                                                                                                                            <  1:323062/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaag                                                                                                                           <  8:316036/137‑6 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaag                                                                                                                           <  8:238447/137‑6 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaag                                                                                                                           <  3:272217/137‑6 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaa                                                                                                                            <  8:66381/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcc                                                                                                                          >  5:107761/1‑139 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcc                                                                                                                          >  5:44401/1‑139 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGc                                                                                                                           <  2:288660/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  4:54762/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  8:208642/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  1:88284/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  8:183976/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  5:9182/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  2:194870/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  7:52835/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCt                                                                                                                       <  1:104166/139‑1 (MQ=255)

              ctctATTGAACCAGCTTCCGTAACAACGAGAATTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  6:245480/138‑1 (MQ=255)

                ctATTGAAGCAGTTTCCGTCACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGGTTTTTTTCATATTTTTTTCTCAGACCGACTTAGATGCGGCTGTC‑AAACAAAAAACAAGCCCATTa                                                                                                                     >  4:217691/1‑139 (MQ=255)

                ctATTGAAGCAGTTTCCGTAACCACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCtt                                                                                                                      >  2:14866/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTa                                                                                                                     >  1:18358/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTa                                                                                                                     >  3:139068/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTa                                                                                                                     >  4:255753/1‑138 (MQ=255)

                   ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGc                                                                                                                           <  2:164912/130‑1 (MQ=255)

                   ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGc                                                                                                                           >  1:164912/1‑130 (MQ=255)

                   ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTaaa                                                                                                                   >  2:5479/1‑138 (MQ=255)

                     gAAGCAGTTTCCGTAACAACGAGATTTATAATATAACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATa                                                                                                                 <  8:291605/138‑1 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGCACAAATAGAAGTGGCTATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAATAAGAGGCtt                                                                                                          >  6:158055/1‑139 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTCAAAAA‑AAAAACAAGCCCCTTAAATAggg                                                                                                              <  2:334440/135‑1 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaa                                                                                                                            <  6:3957/120‑5 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaa                                                                                                                            >  5:3957/1‑116 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAggg                                                                                                              >  1:334440/1‑135 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCtt                                                                                                          >  4:141586/1‑139 (MQ=255)

                            tttCCTTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTg                                                                                                         <  7:12810/139‑1 (MQ=255)

                            tttCCGTAACAACGATATTTATAATATCACTAACAAATAGAAGTGGAAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACAAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTg                                                                                                         <  4:221163/139‑1 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTg                                                                                                         <  8:138398/139‑1 (MQ=255)

                             ttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTg                                                                                                         <  1:195020/138‑1 (MQ=255)

                               ccGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCa                                                                                                       <  8:160543/138‑1 (MQ=255)

                               ccGTAAAAACGAGATTTAAAATATCACGAAAAAATAGAATGGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATCGGGGCTTGCa                                                                                                       <  6:34615/138‑1 (MQ=255)

                                 gTATCAACGAGATTTATAATATCAAGAACAAATAGAAGTGTCAATAACTTTTTTGGTTTTTTTTCATATTTATTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                    <  2:307737/139‑1 (MQ=255)

                                 gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                    <  1:191950/139‑1 (MQ=255)

                                 gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                    <  5:255009/139‑1 (MQ=255)

                                 gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                    <  3:149802/139‑1 (MQ=255)

                                 gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                    <  3:315914/139‑1 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCCGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGCCATAtt                                                                                               >  6:137944/1‑139 (MQ=255)

                                          agaTTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAt                                                                                           <  3:4961/139‑1 (MQ=255)

                                               tataATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        >  6:141663/1‑105 (MQ=255)

                                               tataATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGcccc                                                                                                                        <  5:141663/105‑1 (MQ=255)

                                                 taataTCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCTTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTc                                                                                    <  1:69986/139‑1 (MQ=255)

                                                   atatCACGCACAAATAGAAGTGGCAAAAACTATTTTGGTGTTTTTTCATATTTTTTACTCAGACCAACTTAAATGCGGCCTTC‑AAACAAAAAACACGCCCCTTAAATGGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  2:264797/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTCAAAAA‑AAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  8:53304/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  5:300249/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  2:10627/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  8:291078/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  1:5479/139‑1 (MQ=255)

                                                    tatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCa                                                                                 >  7:309277/1‑139 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc                                                                                <  3:135892/138‑1 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                               >  8:3013/1‑139 (MQ=255)

                                                       cACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaa                                                                                                                            <  2:64376/92‑5 (MQ=255)

                                                       cACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaaaacaa                                                                                                                            >  1:64376/1‑88 (MQ=255)

                                                       cACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACgg                                                                              >  8:111422/1‑139 (MQ=255)

                                                         cGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTa                                                                            <  3:162371/139‑1 (MQ=255)

                                                                  aGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCg                                                                   <  3:141586/139‑1 (MQ=255)

                                                                         gCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACa                                                            >  5:347131/1‑139 (MQ=255)

                                                                         gCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGAAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACa                                                            >  6:268718/1‑139 (MQ=255)

                                                                          cAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAt                                                           <  4:139068/139‑1 (MQ=255)

                                                                             tAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  4:92029/113‑1 (MQ=255)

                                                                             tAACTTTTTTGGTTTTTTTTCATATTTTTTTATCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  >  3:92029/1‑113 (MQ=255)

                                                                                      tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                                <  2:18358/138‑1 (MQ=255)

                                                                                      tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                                <  7:3013/138‑1 (MQ=255)

                                                                                                   tatTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGAc                                  <  2:229771/139‑1 (MQ=255)

                                                                                                   tatTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGAc                                  <  1:14866/139‑1 (MQ=255)

                                                                                                   tatTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGAc                                  <  3:255753/139‑1 (MQ=255)

                                                                                                   tatTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATAATCAATTTAGTTCGCACGGGACACACTGCGAAGGACATTTGTTTGTGAGCGGGCTGGACCGAc                                  <  3:186330/139‑1 (MQ=255)

                                                                                                     tttttttCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACag                                <  1:25311/139‑1 (MQ=255)

                                                                                                                gACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  >  7:30059/1‑78 (MQ=255)

                                                                                                                gACCAACTTATATGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  <  8:30059/78‑1 (MQ=255)

                                                                                                                          tatGCGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATg            <  5:200839/138‑1 (MQ=255)

                                                                                                                              cGGCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt       <  3:156467/139‑1 (MQ=255)

                                                                                                                              cGGCTTCC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCg                 <  7:53802/129‑1 (MQ=255)

                                                                                                                              cGGCTTCC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCg                 >  8:53802/1‑129 (MQ=255)

                                                                                                                                gCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                  >  1:267371/1‑62 (MQ=255)

                                                                                                                                gCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGa                      >  7:106291/1‑122 (MQ=255)

                                                                                                                                gCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGa                      <  8:106291/122‑1 (MQ=255)

                                                                                                                                gCTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt       >  4:156467/1‑137 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTacaca                                                                        >  3:48530/1‑71 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTacaca                                                                        <  4:48530/71‑1 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGAc                                      <  5:139174/105‑1 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGAc                                      >  6:139174/1‑105 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACag                                <  1:246499/111‑1 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACag                                >  2:246499/1‑111 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg    >  6:228870/1‑139 (MQ=255)

                                                                                                                                 cTTTC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg    >  5:191466/1‑139 (MQ=255)

                                                                                                                                   ttC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCt  >  7:5017/1‑139 (MQ=255)

                                                                                                                                   ttC‑AAACAAAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCt  >  1:286023/1‑139 (MQ=255)

GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAAC‑AAAAACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCT  >  BS168_reference_genome/4155253‑4155521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`