| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 4,153,115 | G→T | 100% | L192I (CTT→ATT) | walK ← | two‑component sensor histidine kinase [YycG] |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 4,153,115 | 0 | G | T | 100.0% | 133.4 / NA | 39 | L192I (CTT→ATT) | walK | two‑component sensor histidine kinase [YycG] |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/26); total (13/26) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGCGGAAAT > BS168_reference_genome/4152985‑4153247 | tcatGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAAc < 6:41343/139‑1 (MQ=255) tCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCtgtg > 4:74208/1‑139 (MQ=255) cATACTTTTAAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGc < 1:243223/138‑1 (MQ=255) cagatttttAACCTTCCTCGATAAATTCCCTTTCGCAAGCTACATCGCCTGTTTTCTCATATCTGAATGCGTGTTGTTATTGTTTCTTTCAAGAAAAATACCGAGATGAGCCGTCAAAACAATTGCCAAACCTGTGACg < 4:368093/135‑1 (MQ=255) aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 3:132879/139‑1 (MQ=255) aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 3:74208/139‑1 (MQ=255) tACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCgg > 3:260773/1‑139 (MQ=255) aCTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 1:363839/137‑1 (MQ=255) aCTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg > 2:363839/1‑137 (MQ=255) tttAACCTTCCTCGAGAAATTCGCTTTCGCAAGCTGCTTCGCATGCTTTCGCATATCTGATAGCGGGTGGGTAATGGTTCTTGCCAGATTAATTCATATTATAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc < 2:52029/138‑1 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc < 1:353052/138‑1 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc > 1:52029/1‑138 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc > 3:125195/1‑138 (MQ=255) tAACCTTCCTCGAGAAATTCCATTTCTCAAGCTCCATCGCCTTCTTTCGCATATCTGAACGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCAAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCa < 8:231226/138‑1 (MQ=255) aaCCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAg > 3:366084/1‑138 (MQ=255) tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATctg > 3:368093/1‑137 (MQ=255) gagaAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa < 3:91625/139‑1 (MQ=255) gagaAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa < 3:306817/139‑1 (MQ=255) cTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCAt < 7:181558/139‑1 (MQ=255) aaGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa < 1:133079/139‑1 (MQ=255) cATCGCCTGCTTTCGCATTTCTGAAAGCGGGTGGGTAATGGTTCTTGCCTGTATTATACCGAGAAGAGCCGTCTAAACAATTGCCATACCTGTGCCGGACTCCTGTATGGTGTTGATCGTCTTCATTTGATTAAATACa < 6:278055/139‑1 (MQ=255) tCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc < 3:215346/136‑1 (MQ=255) tCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc > 4:215346/1‑136 (MQ=255) gCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCtt < 4:125195/139‑1 (MQ=255) ttCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt > 2:224488/1‑139 (MQ=255) cATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg < 5:133833/139‑1 (MQ=255) aatatCTGACAGCGGGTGGGTAATGGTTCTTTCCAGAAAAATACCGAGAAGAGCCGTCAAAAAAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg < 6:33003/138‑1 (MQ=255) atatCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGa < 4:260773/139‑1 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTTCCAAACCTGTGCCGGAAGCCAGTATCGTGTTTATCGTCTTCAGTTGATTGAAGCCATCTTCCATACTCGCGACAACGGAGATcgcg > 3:13931/1‑139 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGTTCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg > 5:312868/1‑124 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTTATTAAAGACATCTTCCATACTCGCGACAACGTAGATcgcg < 5:325076/139‑1 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg < 6:312868/124‑1 (MQ=255) tAATGGTTCTTGCCAGAATAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCcac < 8:125999/139‑1 (MQ=255) cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCtt > 3:99373/1‑139 (MQ=255) tGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATc < 7:221036/121‑1 (MQ=255) tGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATc > 8:221036/1‑121 (MQ=255) aGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt < 2:193381/139‑1 (MQ=255) cGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTcgcg < 1:224488/139‑1 (MQ=255) aaaCAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGCGGAAAt < 4:99373/139‑1 (MQ=255) | TCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGCGGAAAT > BS168_reference_genome/4152985‑4153247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |