BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,735	A→T	9.7%	intergenic (+20/‑136)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,735	0	A	T	9.7%	15302.1 / inf	5336	intergenic (+20/‑136)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (286/4474); new base T (557/18); total (843/4493)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5433 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3597‑3872

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTg                                                                                                                                     <  1:295743/139‑1 (MQ=255)

       taaGTTTCATCAAGCAATGAAACACGACAAAGTAAACAATTTAAGTACCATTTCTTATGAGCATGTATTGTATATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTgg                                                                                                                                    <  8:145303/137‑1 (MQ=255)

        cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGa                                                                                                                                   <  1:70306/139‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGa                                                                                                                                   <  5:158939/138‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGa                                                                                                                                   <  7:129583/138‑1 (MQ=255)

           tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGACGC‑TTTTT‑TTTATTTAGAAAg                                                                                                                                >  4:237669/1‑139 (MQ=255)

              catcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTa                                                                                                                             <  3:7167/139‑1 (MQ=255)

               atcaAGCAAAGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTa                                                                                                                             <  4:103632/138‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTaca                                                                                                                           <  7:178855/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTaca                                                                                                                           <  4:214278/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTaca                                                                                                                           <  6:56680/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTaca                                                                                                                           <  8:173161/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACg                                                                                                                         <  2:142437/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACg                                                                                                                         <  3:226665/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACg                                                                                                                         <  2:323326/139‑1 (MQ=255)

                   aGCAATGAAACACTCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGt                                                                                                                        <  7:157541/139‑1 (MQ=255)

                     cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTAATATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTATAAAATT‑GAAAGTTAAAAGtt                                                                                                                       >  5:75642/1‑138 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACt                                                                                                                    <  6:20411/139‑1 (MQ=255)

                           aaCACGCCATAGTAAACAATTTAATTACCATTACTTATGAGGAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGTAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAg                                                                                                                <  7:51111/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  1:254591/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  7:73001/139‑1 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTTGAAAATTAAAAGTTTCTaaaactgta                                                                                                           >  6:327604/1‑133 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTT‑TTAATTTTGTAAGTTACACGTTTCTAAATGGTAt                                                                                                           >  6:154209/1‑139 (MQ=255)

                                  cAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTT‑TTAATTTTTAAAATTTAAAGTTTAAAAAGGGAAttg                                                                                                         >  3:183218/1‑137 (MQ=255)

                                      gacagcAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                     <  3:321746/134‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAAGGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                     <  4:18153/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  1:100028/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  2:264561/139‑1 (MQ=255)

                                         aaCAATTTAAGTACCAATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                  <  1:172513/139‑1 (MQ=255)

                                          aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TTAATTTTTTAATTTTCAAGTTACTAAATGGAATTGAGATAAat                                                                                                 >  2:101205/1‑139 (MQ=255)

                                           cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGATATAATTCTATTAGTCGC‑ATTTT‑TAAATTTCGACAGTTACACGTTACTAAAGGGAATTGAGATAAatt                                                                                                <  6:244043/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTT‑TAATTTTGTAACATTAAACTTTA‑TAAATGGAATTGAGTTAAattatt                                                                                             >  4:150564/1‑139 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  5:13465/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  1:22778/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  4:294834/139‑1 (MQ=255)

                                              tttAACTACCATTACTTATGAGCAAGTATTGTCTAATTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  3:352275/139‑1 (MQ=255)

                                                tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAAttatta                                                                                            <  1:319113/138‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  4:358817/139‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  8:361659/139‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  4:268532/139‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  7:315253/139‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTA‑TAGata                                                                                        <  3:298646/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  6:131117/139‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTTATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGTGAATGGAGATAAATTATTAGAtata                                                                                      <  3:273192/138‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTT‑TAAATTTGAAAATTTAAACGTT‑CTTAAGTGTAATGAATTTAATTATtatatataa                                                                                     >  2:210083/1‑138 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  5:180862/138‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  5:310730/138‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  7:360182/138‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                               <  2:293667/139‑1 (MQ=255)

                                                              tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTT‑TAAATTTGTAAATTAAAACGTT‑CTAAATGGTATTTGTATAAAATATTTTATataataaggac                                                                              >  8:205279/1‑133 (MQ=255)

                                                               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  8:333604/137‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  8:260689/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  1:12211/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  1:53939/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  2:174179/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGGTATCTATTATTTCACGGGAGGAAATACTTCTATGAGTCGC‑TTTTG‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  6:177069/139‑1 (MQ=255)

                                                                      aGTATTGTCTATTTTTAATAGTTTTCTATTATTTATCGGGAGTTAATAATTCTATGAGTCGC‑TTTTT‑TAATTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                     <  2:346605/139‑1 (MQ=255)

                                                                      aGTATTGTCTATTATTAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTTCACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                     <  8:287403/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTTGAAAGTTACACGATTCTAAAGGGAATGGAGATAAATTATTTTATTTACTACTGACAGCttac                                                                       >  7:251783/1‑134 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  2:2383/139‑1 (MQ=255)

                                                                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  1:354632/138‑1 (MQ=255)

                                                                          ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                 <  6:355344/139‑1 (MQ=255)

                                                                            gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                <  1:145389/138‑1 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                              <  8:30881/139‑1 (MQ=255)

                                                                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  4:125795/139‑1 (MQ=255)

                                                                               cagttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  5:98259/136‑1 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                           <  7:29503/138‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         >  5:334001/1‑139 (MQ=255)

                                                                                  ttttAATAGTTATCAATTATTTACCGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGACATAAATTATTATATATACTACTGACAGCTTCCACGAAGCTAAaga                                                          <  5:52660/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTTACTGGAGGATATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  7:227521/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  5:287958/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTACCAAGAAGCTAAagag                                                         <  3:205998/138‑1 (MQ=255)

                                                                                                     tttAACGTGAGGAAATAATTCTATGAGTAGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGTTATACTACTGACAGCTTCCAAGAAGCTATAGAGGTCCCTAGCGCCTACggg                                       <  5:206087/138‑1 (MQ=255)

                                                                                                        aaCGGGAGGAAATAATTCTATGAGTCGC‑GTTTT‑TTAATTTTGTAAGTTACACGTTACTAAATGGAATGGAGATAAATTTTTATATATAATACATACAGCTTACAACAAGCTAAAGAGGTCCCGAGCGCCCAACGGGAAt                                   >  2:48356/1‑139 (MQ=255)

                                                                                                        aaCGGGAGGAAATAATTCTATGAGTCGC‑GTGTT‑TAAAATTTGTTATTTTAACGTAAATAAAGTGATATGAGATTAAATATTTTTTATAAAACTTACAACTTACAAGAAGCAAGAAACGTCACCCACGCCAACGGGGagt                                   >  4:84235/1‑137 (MQ=255)

                                                                                                          cGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TTAATTTTGAAAGTTACACGTTACTAAATGGAATTGAGATAAATTATTATATATAATAATGACACCTTGCAAGAAGCTAAATAGGTCCCTAGCGCCTACGGGGAAttt                                 >  3:162507/1‑139 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                         <  6:78933‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                         <  5:358977‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                         <  2:77850‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTATATGAGTCTG‑TTTTT‑TAAATTTGTAAAGTTACACGTTATTAAAGTTAATGGAGATAAATTTTTAGATATACTAATGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtac                          <  4:60121‑M1/138‑7 (MQ=255)

                                                                                                                      atTCTATGAGTCGA‑TTTTT‑TAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                     <  7:91698‑M1/139‑12 (MQ=255)

                                                                                                                            ttagtCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:273103‑M1/137‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATATATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                  <  3:25127‑M1/136‑15 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:107424‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:201879‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaa                 <  5:105657‑M1/137‑16 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATAAGATATACTACTGACAGCTGCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  6:268234‑M1/139‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTTAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  4:326624‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  8:81745‑M1/138‑18 (MQ=255)

                                                                                                                              aGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  8:116533‑M1/139‑20 (MQ=255)

                                                                                                                                  gC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag          <  7:337384‑M1/138‑23 (MQ=255)

                                                                                                                                      tttt‑tAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAGTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  3:4536‑M1/138‑26 (MQ=255)

                                                                                                                                       ttt‑tAATTTTGGAATGATACTCGTTACTATAGGTAATGGATATAAATTATTAGATATACTACTGACAGCATCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  3:134444‑M1/137‑26 (MQ=255)

                                                                                                                                       ttt‑tAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCGAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  6:298656‑M1/137‑26 (MQ=255)

                                                                                                                                       ttt‑tAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  5:233723‑M1/137‑26 (MQ=255)

                                                                                                                                       ttt‑tAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  1:202436‑M1/137‑26 (MQ=255)

                                                                                                                                       ttt‑tAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  8:57200‑M1/137‑26 (MQ=255)

                                                                                                                                        tt‑tAAATTTGGAAAGTTACACGTTACTATAGGGAATTGAGATAAATTCTTAGATATACTACTGACAGCTTCCTACAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtactttgaaaaaggaagagtatga     <  3:108920‑M1/138‑28 (MQ=255)

                                                                                                                                            aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta  <  8:232900‑M1/138‑31 (MQ=255)

                                                                                                                                            aaaTTTGGAAAGTTAAACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta  <  5:264031‑M1/138‑31 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTT‑TAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3597‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`