Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 6,006 T→G 6.2% V40G (GTT→GGT)  lacZalpha → /vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported6,0060TG 6.2% 16024.5 / 197.0 6500V40G (GTT→GGT) lacZalpha/vntifkey=4
Reads supporting (aligned to +/- strand):  ref base T (2812/3115);  new base G (489/55);  total (3322/3178)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6536 total aligned reads displayed.

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144
                                                                                                                                           |                                                                                                                                           
 tttCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTCCCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGt                                                                                                                                             >  8:19857/1‑138 (MQ=255)
    cacacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTAcc                                                                                                                                         >  1:3059/1‑139 (MQ=255)
         aGGAAACATCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAAGGGGAAAACCC‑TGGCCTTACCCaaat                                                                                                                                    >  8:334864/1‑137 (MQ=255)
            aaaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTaa                                                                                                                                 <  1:12670/139‑1 (MQ=255)
            aaaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTaa                                                                                                                                 <  1:16279/139‑1 (MQ=255)
                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTAcc                                                                                                                                         >  7:27938/1‑122 (MQ=255)
                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                        <  8:216461/139‑1 (MQ=255)
                     tGACCATGAATACGCCAAGCTTGCATGCGTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGCGCTCGAATTCCATGGCCGTCTTTTTACAACGTCGTGAAGGGGAAAACCC‑AGGCGGAACCCCACTGAATCCCCtggg                                                                                                                        >  6:262712/1‑136 (MQ=255)
                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTgcag                                                                                                                      >  6:199352/1‑139 (MQ=255)
                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTgcag                                                                                                                      <  3:92636/139‑1 (MQ=255)
                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTgca                                                                                                                       <  8:363443/138‑1 (MQ=255)
                           tGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTgcagccc                                                                                                                   >  4:118088/1‑136 (MQ=255)
                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGACAACCC‑TGGCGTTACCCAACATAAGAGCCGTGCCGCACAt                                                                                                                 >  6:150018/1‑139 (MQ=255)
                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTTCCCCACTTAATCGCCTTGCAGCACAt                                                                                                                 >  6:3340/1‑139 (MQ=255)
                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTTCCCAACTTAATCGCCTTGCAGCACAt                                                                                                                 >  5:145227/1‑139 (MQ=255)
                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTAccc                                                                                                                                        >  8:283692/1‑116 (MQ=255)
                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                 >  7:50924/1‑139 (MQ=255)
                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCGTGCAGCACAt                                                                                                                 >  1:49694/1‑139 (MQ=255)
                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGGTACCCAACTTAATCGCCTTGCAGcacct                                                                                                                 >  7:162059/1‑137 (MQ=255)
                             aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                <  8:144174/139‑1 (MQ=255)
                                  gCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTAc                                                                                                                                          <  1:194872/108‑1 (MQ=255)
                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGATTTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGAAAAACC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCCCCACCCCCGTTCg                                                                                                       >  3:113837/1‑139 (MQ=255)
                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGGCGGGGCACACCC‑GGGGGGTACCCCCCGTTATCGCCGTGCCGGCCCTcccccggtcg                                                                                                       >  6:238293/1‑134 (MQ=255)
                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGGTACCCAACTTAATAGCCTTTCAGCGCATCCCCCATTCg                                                                                                       >  4:165936/1‑139 (MQ=255)
                                       gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCTCCGTGCAGCACATCCCCCTTTCGc                                                                                                      >  6:286524/1‑139 (MQ=255)
                                       gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGTAAACCC‑TGGCTATACCCCACTAAATCGCCTTGCAGCACATACCCCGTTAGc                                                                                                      >  7:105846/1‑139 (MQ=255)
                                           gCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  6:52962/139‑1 (MQ=255)
                                           gCATGACTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGGCGGTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  5:126850/139‑1 (MQ=255)
                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  2:231313/138‑1 (MQ=255)
                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  8:258689/138‑1 (MQ=255)
                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  4:335020/138‑1 (MQ=255)
                                                ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCAGGGAAAACCC‑TGGCGTTTCCCAACTTAATAGCCTTGCCGCCCATCCCCCTTTCGCCCGCAGGCg                                                                                             >  4:79038/1‑139 (MQ=255)
                                                ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                             <  4:356751/139‑1 (MQ=255)
                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCCCCTTAATCGCCTTGCCGCACATCCCCCTTTCCCCAGCTGGCGt                                                                                            >  3:325160/1‑138 (MQ=255)
                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGt                                                                                            >  1:277091/1‑138 (MQ=255)
                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑AGGCGGTACCCAACTTAATAGCCATGCAGCCCCTACCCCATTCGCCAGCAggggg                                                                                            >  6:109399/1‑135 (MQ=255)
                                                     aGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATa                                                                                        <  1:99524/139‑1 (MQ=255)
                                                         cGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCg                                                                                     >  4:248741/1‑138 (MQ=255)
                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                   <  8:73828/139‑1 (MQ=255)
                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                   <  5:309221/139‑1 (MQ=255)
                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTAAACGTCGTGCCGGGGGAAACCC‑GGGGGGTACCCCCCATAATACCCATGGAGCACAACCCCCCTTAGCCCGCTGGCGGAATATCGaaa                                                                                  >  2:320635/1‑138 (MQ=255)
                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGCAAACCC‑TGGCGGTACCCCACTTTATCGCCGTGCAGCACATCCCCCTTTCGCCAGCTGGCGTGATTGCGaat                                                                                  >  3:371316/1‑138 (MQ=255)
                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCg                                                                                     >  8:51946/1‑136 (MQ=255)
                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCg                                                                                     >  3:353164/1‑136 (MQ=255)
                                                               tAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑AGGCGGTACCCAACATAAAAGCCATGCAGCACAAACCCCCTTCGCCCCCAGGCCGAATAGCGAAGACGc                                                                              >  4:49027/1‑139 (MQ=255)
                                                                  agGATCCCCGGGTACCGAGCTCGAAGTCACTGGCCGTCGTTTTACAAAGTCGTTCCGGGGTAAACCCGGGGCGTT‑CAACACTTAAACGCCATGCACCACGAACCCCGTTCCCCAGCGGGCGTAAAAGCGAAGGGGCCCg                                                                           >  4:30688/1‑139 (MQ=255)
                                                                     aTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CAc                                                                       >  1:2522/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAAACC‑TGGCGGTTCCCCACATAATCGCCTTGCCGCCCCTCCCCCATTCGCCACCGGGCCGAAAAGCGAAGAGGCCCG‑cccc                                                                      >  2:57982/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACCC‑TGGCGGTACCCCACTTAATAGCCTTGCCGCACATCCCCCATTCGCCACCTGGCGTGATAGCGAAGTGGcccccccc                                                                       >  5:224682/1‑134 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTAcc                                                                                                                                         <  5:357356/73‑1 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCCACTTAATCGCCTTGCCGCCCATCCCCCTTTCGCCAGCTGGCGTTATAGCGAAGAGGCCCG‑cccc                                                                      >  7:358287/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CAcc                                                                      >  6:314411/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                >  5:331513/1‑98 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGGAATAGCGCAGAGGCCCG‑CAcc                                                                      >  6:130817/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CAcc                                                                      >  6:357798/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CAcc                                                                      >  3:247974/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATAGCCTTGCAGCACATACCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑cccc                                                                      >  5:166471/1‑139 (MQ=255)
                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACGGGGAAAACCC‑TGGCGGTACCCAACTTAATCGCCTTGCAGCCCCTCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCG‑CAcc                                                                      >  8:122299/1‑139 (MQ=255)
                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAACAACC‑TGGCGTTACCCAACTTAATCGCCTTACAGCACATCCCCCGTTAGCCACCTGGCGTAATAACGACGAgccccg‑cccc                                                                      >  7:191206/1‑138 (MQ=255)
                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTAccc                                                                                                                                        <  1:70408/73‑1 (MQ=255)
                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCg                                                                     >  3:66175/1‑139 (MQ=255)
                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAAAGTCGTGACTGGGAAAACCC‑AGGCGTTACCCCACATAATCACCTTGCAGAACATACCCCTTTACCCAGCCTGCGGAATAACGAAGACGCCCG‑CAcccc                                                                    >  4:275863/1‑136 (MQ=255)
                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCg                                                                 <  6:80502/139‑1 (MQ=255)
                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGGTTTTCAACGTCGTGACTGGGCAAACCC‑TGGCGGTACCCCCCGTAATCGCCGTGCCCCCCCTACCCCCTTCGCCCGCTCGCGTAATAGCCAACACGCCCC‑CAcccaacg                                                                 >  7:129264/1‑134 (MQ=255)
                                                                           gggTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCg                                                                 <  5:101461/139‑1 (MQ=255)
                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACTACTTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGcc                                                               <  7:96792/139‑1 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACAC‑AGGCGTTAACCAACATAATAGCCAAGCAACACAACCCCCAATCGCCAACAGGCGTAATAACGAAAATGCCCC‑CCACCAACGCCCTTccc                                                         >  4:190581/1‑139 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTAccc                                                                                                                                        >  2:204930/1‑61 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTg                                                                                                                         <  8:171510/76‑1 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                <  7:254475/85‑1 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  7:70904/99‑1 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  5:60084/99‑1 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTccc                                                         >  7:231287/1‑139 (MQ=255)
                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACAACCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CCCCGCTCGCCCGTcc                                                          >  3:139165/1‑138 (MQ=255)
                                                                                        gAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAg                                                    <  7:328424/139‑1 (MQ=255)
                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTgcgc                                              <  3:277756/139‑1 (MQ=255)
                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTgcgc                                              <  8:310289/139‑1 (MQ=255)
                                                                                                tGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAg                                            >  3:182346/1‑139 (MQ=255)
                                                                                                tGGCCGTCGTTTTACAACGGCGTGACTGGGAAAACCC‑AGGCGTTACCCAACTTAATCGCCGTGCAGCACCTACCCCATTCGCCCGCGGGCGGAATAGCGCAGAGGCCCG‑CCCCCCGCGCCCGGCCCAACAGTTgcgccg                                            >  2:290831/1‑137 (MQ=255)
                                                                                                         ttttACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTAAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatgg                                   <  8:236663/139‑1 (MQ=255)
                                                                                                                 cgtcgtGGCTGGGAAAACCC‑TGGCGTTACCCAACTTAATAGCCGTGCAGCCCCTCCCCCATTCGCCAGCTGGCGTTATAGCGAATAGGCCCGCCCCCCATCGCCGTTCCCAACAGTTGCGCGGCCTcaatggcgattgg                            >  2:83836/1‑139 (MQ=255)
                                                                                                                 cgtcgtGACTGGGTAAACCC‑TGGCGTTAACCAACTTAAACGCCTTGCCGCACATCCCCCATTCGCCCGCGGGCGTAATAGCGAAGAGGCCCG‑CACCCCTCGCCCTTCCCACCACTTGCGCAGCCTgaatggcgcatggc                           >  2:127677/1‑139 (MQ=255)
                                                                                                                 cgtcgtGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTCGCCAGCGGGCGTAATAGCGAAGAGGCCCC‑CACCCCTCGCCCGTCCCCACAGTTGCGCAGCCTgaatggcgaaaggc                           >  5:279532/1‑139 (MQ=255)
                                                                                                                 cgtcgtGACAGGGTAAACCC‑TGGCGTTACCCAACATAATCGCAGTGGAGCACAACCCCCGTTCGCCAGCTGGCGGAAAAGCGTAGAGGCCCC‑CCCCCAACGCCCGTCCCACCATGTGCGCAGCCAgaatggcgactggc                           >  4:219676/1‑139 (MQ=255)
                                                                                                                   tcgtGACTGGGAAAACCC‑TGGCGTTACCCAACTTAAt                                                                                                                                <  5:253504/37‑1 (MQ=255)
                                                                                                                      tGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCGTGCAGCACATCCCCCTTTCGCCAGCGGGCGGAATAGCGAAGAGGCCCG‑CCCCGATCGCCCGTCCCCTCAGTTGCGCCGCCTGAATGGCGCATGGCGCCTg                      >  2:172354/1‑139 (MQ=255)
                                                                                                                            ggAAACCCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  5:189434/137‑1 (MQ=255)
                                                                                                                            ggAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCGCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  1:224132/137‑1 (MQ=255)
                                                                                                                            ggAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  2:25917/137‑1 (MQ=255)
                                                                                                                            ggAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  4:142312/138‑1 (MQ=255)
                                                                                                                            ggAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  1:100040/138‑1 (MQ=255)
                                                                                                                            ggAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  7:212186/137‑1 (MQ=255)
                                                                                                                             gAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGt               >  1:350265/1‑139 (MQ=255)
                                                                                                                             gAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CCCCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatggc                           >  6:172968/1‑127 (MQ=255)
                                                                                                                             gAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                              <  5:81685/124‑1 (MQ=255)
                                                                                                                                    c‑TGGCGTTACCCAACTTAATCGCCTTGCTGAACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  3:295876/139‑1 (MQ=255)
                                                                                                                                      tGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGAGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATCCGGTATTTtctc       >  2:37872/1‑139 (MQ=255)
                                                                                                                                       ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc      >  3:216521/1‑139 (MQ=255)
                                                                                                                                        gCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGATGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCt     >  3:16108/1‑139 (MQ=255)
                                                                                                                                          gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa   <  7:300906/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC‑TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG‑CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑