Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 6,277 A→C 5.7% intergenic (+66/–) lacZalpha → / – /vntifkey=4/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported6,2770AC 5.7% 21587.7 / 211.9 7819intergenic (+66/–)lacZalpha/–/vntifkey=4/–
Reads supporting (aligned to +/- strand):  ref base A (3514/3713);  new base C (561/14);  total (4082/3737)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7892 total aligned reads displayed.

CCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTT  >  Exported/6139‑6415
                                                                                                                                          |                                                                                                                                            
  ttACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGTCGGGCGTGTCTGCTACCAGGATc                                                                                                                                            >  2:145559/1‑139 (MQ=255)
    aCGCATCTGTGCTGTATATCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                            <  1:87384/137‑1 (MQ=255)
    aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                            <  8:356548/137‑1 (MQ=255)
    aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                            <  2:62039/137‑1 (MQ=255)
    aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAAACTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACACGCTGACGCGCCCTGACGGGCTTGTATGCTCCCGGCaaacc                                                                                                                                          >  5:125758/1‑135 (MQ=255)
        aTCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                                                      <  8:306291/139‑1 (MQ=255)
             tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                  <  6:29615/138‑1 (MQ=255)
             tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                  <  5:148165/138‑1 (MQ=255)
                  tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCt                                                                                                                            >  7:280688/1‑139 (MQ=255)
                  tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCATCCGCTTACGGACAAGCt                                                                                                                            >  4:311740/1‑139 (MQ=255)
                        acacCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAcc                                                                                                                      <  6:315815/139‑1 (MQ=255)
                         ccccGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAc                                                                                                                       <  2:293304/135‑1 (MQ=255)
                         agccGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCg                                                                                                                     <  1:130472/137‑1 (MQ=255)
                            cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGtct                                                                                                                  >  1:300310/1‑139 (MQ=255)
                              cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                                                >  7:256398/1‑139 (MQ=255)
                              cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCCGACCGGCGTGTCTGCTCCCCGCCTCCCCTTACAGACAACCGGTGACCggcgac                                                                                                                >  6:14731/1‑134 (MQ=255)
                                  tGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                             <  7:131679/138‑1 (MQ=255)
                                    gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCCGCCCCGACACCCGCCCACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGc                                                                                                          >  8:119156/1‑139 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCCACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCCTCCCCGTTCAGACAAGCTGGGTCCGTCGCCGGGGGcgg                                                                                                        >  5:46220/1‑137 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGATGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                        <  8:252716/123‑1 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                  <  3:148542/113‑1 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                  >  8:227095/1‑113 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                                 <  7:105574/114‑1 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                                        >  6:345710/1‑139 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCCTCCGCGTACAGCCAAGCAGTGACCGTCTCCGGGGGcgg                                                                                                        >  3:79646/1‑137 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCCGACGGGCGTGTCTGCTCCCGGCCTCCGCATAAAGACAAGCAGTGCCCCTCTCCGGGCGCTg                                                                                                        >  4:206685/1‑139 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCGTGTCTGCTACCGGCAACCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                                        >  3:116094/1‑139 (MQ=255)
                                      gCACTCTCAGTACAATCTGCTCTGATCCCGCATAGTTAAGCCAGCCCCGACACCCGACAACACCCGCTGACGCGCCCTGACCGGCTTGTCTGCTACCGGCAACCGCTTACCAAAAAGCTGTGAACCTCTCCCCGAGcag                                                                                                        >  2:266129/1‑137 (MQ=255)
                                       cACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                                 <  1:16373/113‑1 (MQ=255)
                                        aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGCCGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCATACAGACAAGCTGTGCCCGTCTCCGGGAGCTg                                                                                                        >  4:285050/1‑137 (MQ=255)
                                        aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCTCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑a                                                                                                     <  1:219450/139‑1 (MQ=255)
                                        aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGCCCGTCTCCGGGAGCTGC‑a                                                                                                     >  4:104871/1‑139 (MQ=255)
                                        aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGCCCGTCGCCGGGAGCGGC‑a                                                                                                     >  6:1003/1‑139 (MQ=255)
                                              aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATcc                                                                                                                                           <  7:155459/96‑1 (MQ=255)
                                              aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                                        <  5:328327/99‑1 (MQ=255)
                                                 aCAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCa‑g                                                                                            <  4:76424/138‑1 (MQ=255)
                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCGACCCGCCTCCGCTTACATCCAACCTGTGCCCGTCTCCGGGACCGGC‑ATGTGTCA‑GAGGt                                                                                        >  7:231149/1‑139 (MQ=255)
                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGCCGGGCTTGTCAGCTCCCCGCATCCGCTTACAGACAACCAGTGACCGTCTCCCGGCGCTGC‑ATGTGTCA‑GAggg                                                                                        >  3:220635/1‑138 (MQ=255)
                                                     tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCa                                                                                                                                              <  2:118370/86‑1 (MQ=255)
                                                     tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGtt                                                                                       >  5:102760/1‑139 (MQ=255)
                                                      cTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACCCCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCCTCCGCTTACAGACAAGCGGTGACCGTCTCCGGGCGCGGC‑ATGTGTCA‑GAGGgtt                                                                                      >  2:240263/1‑139 (MQ=255)
                                                      cTGCTCAGATGCCGCATAGATAAGCCAGCCCCGCCACCCGCCCCCACCCCCTGACGCGCCCTGACGGGCGTGTCTGCTCCCCGCAACCGCATACAGCCAAGCTGTCCCCCTCTCCCGGCGCTGC‑CAGTGTCA‑GCGCttt                                                                                      >  8:198648/1‑139 (MQ=255)
                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGCCAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGtttt                                                                                     >  7:49849/1‑138 (MQ=255)
                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑aa                                                                                                    <  6:269661/124‑2 (MQ=255)
                                                         ctctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGtttt                                                                                     <  5:196600/137‑1 (MQ=255)
                                                         ctctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCa                                                                                   >  5:75154/1‑139 (MQ=255)
                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCg                                                                                                                     >  7:355920/1‑106 (MQ=255)
                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGtctc                                                                                                                 <  8:76063/110‑1 (MQ=255)
                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                           <  8:336645/116‑1 (MQ=255)
                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCAc                                                                                  >  1:62998/1‑139 (MQ=255)
                                                              aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGt                                                                               >  7:334286/1‑138 (MQ=255)
                                                              aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGt                                                                               <  5:260754/138‑1 (MQ=255)
                                                                 ccGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGtcctc                                                                           >  6:78359/1‑139 (MQ=255)
                                                                   gCATAGTTAAGCCAGCCCCGACACCGGCCAACACCCGCTGACGCGCCCGGTCGGGCTTGTCTGCGCCCGGCCTCCGCTTAAAGCCAAGCTGTGCCCGGAAGCGGGTTCTGCAATGTG‑CA‑TCTGTTTTCACCGTCATAAc                                                                         >  2:330625/1‑139 (MQ=255)
                                                                   gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGGCGGGCTGGTCATCTCCACGCCACCCCTTACAGACAAGCAGAGACCCGCAACGGGAGCAGC‑ATGTGTCACGAGG‑TTTCACCCtcatctc                                                                         >  6:121880/1‑137 (MQ=255)
                                                                   gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCa‑gag                                                                                          <  4:180486/122‑1 (MQ=255)
                                                                   gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCAc                                                                         >  7:179305/1‑139 (MQ=255)
                                                                        gTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTACCGGCCTCCGCTTTAAGGAAAGCTGTGACCGTGTCCGGGCGCTGC‑CTGTGTCA‑GAGGTTTTGACCGGCCTCACCGaa                                                                     >  3:191056/1‑138 (MQ=255)
                                                                           cagccagccCCGACCCCCGCCACCTCCCGCTGCCGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGGCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAAcg                                                                  <  3:55423/137‑1 (MQ=255)
                                                                              ccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                        <  7:71015/83‑1 (MQ=255)
                                                                               cagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCga                                                             <  1:196061/139‑1 (MQ=255)
                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGCCAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                                           >  3:166953/1‑139 (MQ=255)
                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                                 >  4:134373/1‑71 (MQ=255)
                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑a                                                                                                     <  1:272904/98‑1 (MQ=255)
                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                                           >  3:369990/1‑139 (MQ=255)
                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                                           >  1:100101/1‑139 (MQ=255)
                                                                                  ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATACGCTTACAGACAATCTGTGACCGTCTCCGGGCGCTGC‑ATGTGTCA‑GAGGGTTTGACCGTCATCACCGAAACGCGCGAGAc                                                          >  5:284580/1‑139 (MQ=255)
                                                                                   cccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCCGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACg                                                         >  2:21525/1‑139 (MQ=255)
                                                                                      gACCCCCGCCAACACCCGCTGCCGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaaa                                                      <  1:332068/139‑1 (MQ=255)
                                                                                       acacCCGCCAACACCCGCTGACGCGCCCAGACGGGCTTGTCTGCTCCCCGCATCCGCTTACAGACAAGCTGTGACCCTCTCCCGGAGCTGC‑ATGTGTCA‑GAGGTTTTAACCCTCATCACCCAAACGCGCGAGACCaaat                                                     >  4:52065/1‑138 (MQ=255)
                                                                                                 acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                                           <  6:179046/139‑1 (MQ=255)
                                                                                                        cTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCt                                    <  7:94702/139‑1 (MQ=255)
                                                                                                           aCGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GATGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTa                                   <  1:245379/137‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGTCCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  3:347200/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  3:271871/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  8:279807/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  4:358160/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  3:298371/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  2:4008/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  3:321641/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  4:237625/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTTGTCTGCTCCAGTCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGA‑ATGTGTCA‑GATGTTTTCACAGTCATCACCTAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  8:508/139‑1 (MQ=255)
                                                                                                               gcCCTGACGGGCTGGTCTGCTCCCGGCATCCGCGTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGGGGCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  4:261544/139‑1 (MQ=255)
                                                                                                                    gACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGt                        <  8:174496/139‑1 (MQ=255)
                                                                                                                       gggCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTtat                            >  6:208518/1‑132 (MQ=255)
                                                                                                                        ggCTGGTCTGCTCCCGTCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAAt                    <  6:44546/139‑1 (MQ=255)
                                                                                                                         gCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCCCCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATg                   >  5:173186/1‑139 (MQ=255)
                                                                                                                          ctgggcTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                  <  8:151329/134‑1 (MQ=255)
                                                                                                                           ttGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAt                                  <  1:40902/122‑1 (MQ=255)
                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGGGGCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  6:251376/139‑1 (MQ=255)
                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  7:203263/138‑1 (MQ=255)
                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATg              <  2:1936/139‑1 (MQ=255)
                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCAACGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  3:247661/138‑1 (MQ=255)
                                                                                                                               cTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGa             >  5:353529/1‑139 (MQ=255)
                                                                                                                                   tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCAc                                                                                  >  4:331590/1‑66 (MQ=255)
                                                                                                                                   tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAt                                  >  4:157866/1‑114 (MQ=255)
                                                                                                                                   tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  7:307316/119‑1 (MQ=255)
                                                                                                                                   tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGataa          >  5:305411/1‑138 (MQ=255)
                                                                                                                                       ggCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAACCGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATg     >  2:88094/1‑139 (MQ=255)
                                                                                                                                       ggCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATg     >  5:241010/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGC‑ATGTGTCA‑GAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTT  >  Exported/6139‑6415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑