Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	892,022	A→T	100%	N196I (AAT→ATT)	malR →	transcriptional activator of the Mal operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	892,022	0	A	T	100.0%	255.7 / NA	73	N196I (AAT→ATT)	malR	transcriptional activator of the Mal operon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (33/40);  total (33/40)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCGC  >  BS168_reference_genome/891892‑892157                                                                                                                                   |                                                                                                                                        aatttGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTaaaa                                                                                                                                  >  2:78621/1‑138 (MQ=255) aatttGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTaaaa                                                                                                                                  <  4:147510/138‑1 (MQ=255)        tGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGt                                                                                                                          >  4:107189/1‑139 (MQ=255)          cgcgATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTcc                                                                                                                        <  8:197595/139‑1 (MQ=255)                       aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGa                                                                                                            <  8:221850/138‑1 (MQ=255)                       aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGa                                                                                                            <  7:27376/138‑1 (MQ=255)                       aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGa                                                                                                            <  7:32975/138‑1 (MQ=255)                       aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGAc                                                                                                           <  8:355460/139‑1 (MQ=255)                       aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGAc                                                                                                           <  2:307953/139‑1 (MQ=255)                        aGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAGAACAATGTCCTCGCCCAGCTGACt                                                                                                          <  3:108039/139‑1 (MQ=255)                        aGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACt                                                                                                          <  2:277088/139‑1 (MQ=255)                        aGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACt                                                                                                          <  7:57078/139‑1 (MQ=255)                            aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  5:44438/1‑138 (MQ=255)                            aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  7:24789/1‑138 (MQ=255)                            aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  3:155706/1‑138 (MQ=255)                            aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  8:211009/1‑138 (MQ=255)                            aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  6:148234/1‑138 (MQ=255)                            aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACGCCg                                                                                                       >  7:157861/1‑138 (MQ=255)                             aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     <  6:148757/139‑1 (MQ=255)                             aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     <  4:190522/139‑1 (MQ=255)                             aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     <  8:66165/139‑1 (MQ=255)                             aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     <  1:78621/139‑1 (MQ=255)                              cGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACCATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                    <  6:93610/139‑1 (MQ=255)                                 tCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAAt                                                                                                 >  3:134599/1‑139 (MQ=255)                                   tGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCt                                                                                               >  2:68501/1‑139 (MQ=255)                                    gTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTc                                                                                                         >  2:8074/1‑128 (MQ=255)                                    gTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTc                                                                                                         <  1:8074/128‑1 (MQ=255)                                    gTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCtt                                                                                              >  2:215026/1‑139 (MQ=255)                                    gTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCtt                                                                                              >  1:167013/1‑139 (MQ=255)                                           atcatTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCg                                                                                        <  2:190364/138‑1 (MQ=255)                                               tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGAc                                                                                                           >  5:296728/1‑115 (MQ=255)                                               tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGAc                                                                                                           <  6:296728/115‑1 (MQ=255)                                               tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg                                                                                   >  4:169011/1‑139 (MQ=255)                                               tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTAATCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACaac                                                                                   >  1:207785/1‑138 (MQ=255)                                                                 cacCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAg                                                                 <  4:16071/139‑1 (MQ=255)                                                                            aTTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACg                                                       >  4:335774/1‑138 (MQ=255)                                                                                 tCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAAtt                                                 >  3:332737/1‑139 (MQ=255)                                                                                 tCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAAtt                                                 >  5:358538/1‑139 (MQ=255)                                                                                   aGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt                                               <  2:167013/139‑1 (MQ=255)                                                                                   aGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt                                               >  7:225022/1‑139 (MQ=255)                                                                                          aaTGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCtttt                                        >  8:184834/1‑139 (MQ=255)                                                                                              cTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACACTGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCa                                     <  4:182965/138‑1 (MQ=255)                                                                                               tttCTGCAAAGGGTATTCACTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCAcc                                   >  3:304548/1‑139 (MQ=255)                                                                                                 tCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt                                               <  1:11195/125‑1 (MQ=255)                                                                                                 tCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt                                               >  2:11195/1‑125 (MQ=255)                                                                                                        aGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCa                                                                                 >  5:200318/1‑84 (MQ=255)                                                                                                        aGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCa                                                                                 <  6:200318/84‑1 (MQ=255)                                                                                                           gTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGt                       <  6:44438/139‑1 (MQ=255)                                                                                                             aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                    <  1:327490/60‑1 (MQ=255)                                                                                                             aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                    >  2:327490/1‑60 (MQ=255)                                                                                                             aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTg                     >  6:123510/1‑139 (MQ=255)                                                                                                              ttCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGACAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTg                     <  6:143284/138‑1 (MQ=255)                                                                                                               tCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     >  1:247205/1‑57 (MQ=255)                                                                                                               tCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     <  2:247205/57‑1 (MQ=255)                                                                                                               tCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGc                   >  5:199446/1‑139 (MQ=255)                                                                                                                 ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                  <  1:161248/138‑1 (MQ=255)                                                                                                                 ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                  <  8:225022/138‑1 (MQ=255)                                                                                                                 ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                  <  1:68501/138‑1 (MQ=255)                                                                                                                 ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                  <  7:184834/138‑1 (MQ=255)                                                                                                                 ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                  <  3:201244/138‑1 (MQ=255)                                                                                                                 ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGa                 <  3:107189/139‑1 (MQ=255)                                                                                                                    ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGATCGGAATTTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg               >  6:253101/1‑138 (MQ=255)                                                                                                                    ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGTCGAAg               >  7:75784/1‑138 (MQ=255)                                                                                                                    ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg               >  2:104776/1‑138 (MQ=255)                                                                                                                     tATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCa             >  6:165059/1‑139 (MQ=255)                                                                                                                      aTTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCa             <  7:211009/138‑1 (MQ=255)                                                                                                                      aTTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCa             <  6:199446/138‑1 (MQ=255)                                                                                                                           cATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCAttcttc       <  3:152110/139‑1 (MQ=255)                                                                                                                             ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAATCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     <  5:12655/139‑1 (MQ=255)                                                                                                                             ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTTTTCcg     >  1:190688/1‑139 (MQ=255)                                                                                                                             ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     <  1:215026/139‑1 (MQ=255)                                                                                                                                cGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcgcg   <  3:169011/138‑1 (MQ=255)                                                                                                                                cGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCgcgc  <  4:332737/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        AATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCGC  >  BS168_reference_genome/891892‑892157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑