Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,139,565 A→G 8.3% E1082E (GAA→GAG)  addB → ATP‑dependent deoxyribonuclease (subunit B)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,139,5650AG 8.3% 136.5 / 3.3 48E1082E (GAA→GAG) addBATP‑dependent deoxyribonuclease (subunit B)
Reads supporting (aligned to +/- strand):  ref base A (26/18);  new base G (2/2);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGGCTTGCTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGCACATACTGTGCGTTCAA  >  BS168_reference_genome/1139431‑1139699
                                                                                                                                      |                                                                                                                                      
aaGGGCTTGCTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAg                                                                                                                                     >  1:281596/1‑138 (MQ=255)
        gCTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGAt                                                                                                                            >  1:26652/1‑139 (MQ=255)
        gCTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGAt                                                                                                                            >  7:222401/1‑139 (MQ=255)
             tcGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCtttt                                                                                                                       >  7:240685/1‑139 (MQ=255)
              cGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCtttt                                                                                                                       >  1:191643/1‑138 (MQ=255)
              cGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCtttt                                                                                                                       >  5:362681/1‑138 (MQ=255)
                   aTCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACa                                                                                                                   >  5:18168/1‑137 (MQ=255)
                       ggACGTGGTGCCCCTCCTGGAAACAACCCTTCAAGAGGGACGTTCAAATATCATAACCGCCGGCGTCAAAAACGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCAt                                                                                                             <  8:302230/139‑1 (MQ=255)
                          cgTGGTTCGCAGCATGCATACAACCGTTCAAGTGGGACGTAAAAATATAATAACCGCCGGCTTCAAAAACGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCAtgtg                                                                                                          <  2:212203/138‑1 (MQ=255)
                           gTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAACGCATGTgc                                                                                                         >  5:242606/1‑139 (MQ=255)
                               ttCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTGTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGcgccg                                                                                                     >  7:364357/1‑139 (MQ=255)
                                  gCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCAc                                                                                                  >  6:361675/1‑139 (MQ=255)
                                      cATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTc                                                                                              >  7:368396/1‑139 (MQ=255)
                                                 cccTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCggg                                                                                   >  5:160523/1‑139 (MQ=255)
                                                    ttCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAGAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCg                                                                                 >  7:115142/1‑138 (MQ=255)
                                                    ttCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAGAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCg                                                                                 >  7:109821/1‑138 (MQ=255)
                                                    ttCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCg                                                                                 >  3:284568/1‑138 (MQ=255)
                                                     tCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGaa                                                                               >  2:265549/1‑139 (MQ=255)
                                                      cAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGaa                                                                               >  3:232581/1‑138 (MQ=255)
                                                              aCGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGAGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACATTCCAAGAAGCGGGCGAACAAATCAcc                                                                      >  1:240266/1‑139 (MQ=255)
                                                                 ttCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAATAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGAc                                                                   >  4:108819/1‑139 (MQ=255)
                                                                          cttacaCGCCGGCTTGAAAAACCACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGt                                                           <  8:127702/133‑1 (MQ=255)
                                                                          cATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGCCGGGCGCGTa                                                          >  6:195422/1‑139 (MQ=255)
                                                                                cgccttcTTTAAAAAACACGGCTCTCTCAGATCATACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCAtt                                                    <  8:185036/133‑1 (MQ=255)
                                                                                ccccGGCTTCAAAAACCACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCAtt                                                    <  2:236320/137‑1 (MQ=255)
                                                                                   cGGCTTCAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAg                                                 <  8:166680/139‑1 (MQ=255)
                                                                                                     ctctctCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATgaagaa                                >  7:50589/1‑138 (MQ=255)
                                                                                                            agtacagaCTCAGCATCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGc                        <  8:368396/135‑1 (MQ=255)
                                                                                                            agatcagaCTCAGCAGCAGTCGGTGAGAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGc                        <  8:115142/139‑1 (MQ=255)
                                                                                                            agatcagaCTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGCCGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGc                        <  5:195422/139‑1 (MQ=255)
                                                                                                            agatcagaCTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGAc                          <  4:284568/137‑1 (MQ=255)
                                                                                                            agatcagaCTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGc                        <  5:361675/139‑1 (MQ=255)
                                                                                                            agatcagaCTCAGCAGCAGTCGGGGAAAAGGAATTTGATCTTTTGACAAAGCCTGGGCGCCGCACCTTCCAAGACGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGc                        <  2:282646/139‑1 (MQ=255)
                                                                                                            agatcagaCTCAGCAGCAGTCGGGGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGc                        <  2:191643/139‑1 (MQ=255)
                                                                                                            agataagaCTCAGCAGCAGTCGGTGAGAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGc                        <  8:109821/139‑1 (MQ=255)
                                                                                                               tcagaGTCTGCTGCAGTGGTTGAAAAGTAATTTGATATTTTGACAAAGCTGTTGCGCCGCACGTTCCAAGAAGCGGGCTAAAAAATCACCGTGGGGGGGTTATCCTTTTAGCCGTACAAAATGAAGAACAAGACGCCg                      <  2:240266/138‑1 (MQ=255)
                                                                                                               tcagaCTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAGCAAATCACCGACGGGCGCGTATCCATTGAGCCGGACAAAATGAAGAACAAGACGCCGt                     >  6:165163/1‑139 (MQ=255)
                                                                                                                 agaCTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTg                    <  6:160523/138‑1 (MQ=255)
                                                                                                                  gaCTCAGCTGGAGTCTGTTAAATGGAATTTGATCTTTTGACAAAGCATGTGCGCAGCACCTGCCAATAAGGTGGCGAACAAATCACCGACGGGCGAGTATCCATTGAGCCGTACAAAATGAAGAACAAGACTCCGTGca                  <  6:308201/139‑1 (MQ=255)
                                                                                                                   aCTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGACCAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGca                  >  7:5196/1‑138 (MQ=255)
                                                                                                                   aCTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGcac                 >  4:233444/1‑139 (MQ=255)
                                                                                                                            cagTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGCACATACTGTGc        >  6:144346/1‑139 (MQ=255)
                                                                                                                               tCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGca                  <  2:81662/126‑1 (MQ=255)
                                                                                                                               tCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGca                  >  1:81662/1‑126 (MQ=255)
                                                                                                                               tCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGCACATACTGTGCGtt     >  8:176436/1‑139 (MQ=255)
                                                                                                                                 ggTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATg                                     <  5:74307/105‑1 (MQ=255)
                                                                                                                                 ggTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATg                                     >  6:74307/1‑105 (MQ=255)
                                                                                                                                  gTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCCCCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGCACATACTGTGCGTTCaa  <  8:364357/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
AAGGGCTTGCTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTTGAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCCGCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCGTGCACATACTGTGCGTTCAA  >  BS168_reference_genome/1139431‑1139699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑