Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,690,758 C→A 100% R214S (CGT→AGT)  codY → transcriptional regulator, GTP and BCAA‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,690,7580CA100.0% 186.2 / NA 55R214S (CGT→AGT) codYtranscriptional regulator, GTP and BCAA‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/32);  total (23/32)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAAT  >  BS168_reference_genome/1690638‑1690890
                                                                                                                        |                                                                                                                                    
aTGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTGCTGTTATTGTGATCg                                                                                                                    >  6:237721/1‑139 (MQ=255)
       tCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCa                                                                                                              <  7:20960/138‑1 (MQ=255)
        cAGCTCGCTTTCTTACAGTGAGCTTGAAGCAAATGAGCACATATTTGAGGAGCTTCACGGAAATGAAGTTCTTCTTGTTGAAAGTAAAATTGCTGACCGTGTCGTCATTACCAGTTCTGTTATTGTGAACGCACTCAg                                                                                                             >  3:23644/1‑138 (MQ=255)
         agcgctcTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGCGTTGGTTGACTGAATTGAAGGGCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCa                                                                                                              <  2:186271/130‑1 (MQ=255)
         aGCTCGCGTTCTTTCAGGGAGATTGAAGCTATTGAGCACATTTTTGGGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACGAGTTCTGTTATTGTGAACGCACTCAGaa                                                                                                           <  4:245171/139‑1 (MQ=255)
                  tCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    <  5:175730/137‑1 (MQ=255)
                     tACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcg                                                                                               >  5:212327/1‑139 (MQ=255)
                        aGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGGACTGAAGGTCTGCTTGTTGCAAGTAAAATTGCTGACCGTGGCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg                                                                                            <  8:164179/139‑1 (MQ=255)
                          tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGATCGCACTCAGAAAGCTGGAGAGCGCCTgt                                                                                          >  5:56778/1‑139 (MQ=255)
                          tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCTCTCTGACAGCTGGAGAGCGCCGgt                                                                                          >  7:58769/1‑139 (MQ=255)
                          tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                              >  8:219420/1‑135 (MQ=255)
                          tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                              <  7:219420/135‑1 (MQ=255)
                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    <  2:39712/127‑1 (MQ=255)
                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    >  8:111739/1‑127 (MQ=255)
                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    >  1:39712/1‑127 (MQ=255)
                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                                    <  7:111739/127‑1 (MQ=255)
                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                                        >  6:83794/1‑139 (MQ=255)
                              cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTa                                                                                      >  4:73546/1‑139 (MQ=255)
                                 gAAGCAATTTAGAACATTTTTTAGGAGCTTGACGGAAATGACGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCTTCATTAAAAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                                    <  1:337274/138‑1 (MQ=255)
                                 gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt                                                                                     <  6:123977/137‑1 (MQ=255)
                                            gCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCTAGTATAATTGCTGACGGTGTCGGCCTTACGAGTTCTGTTATTGTGAACGCACTCAGAACGCTGGAGAGCGCCGGTGGTATGGAGTGTAGTTct                                                                        >  2:314860/1‑138 (MQ=255)
                                             cacaTTTTTGAGGAGCTGGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAt                                                                       <  6:61684/139‑1 (MQ=255)
                                              cagtttttGAGGAGCTTGAGGGAAATGAAGGTCTTCTTGTTGCATGTTATTTTGCTGACCGTTTGGGCATTACCAGTTCTGTTATTGTGAACGCACTCTGTAAGCTGGAGAGCGCCGGTGTTATCGAGTGTAGATCAt                                                                       <  7:20789/135‑1 (MQ=255)
                                               caTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTa                                                                     <  6:166016/139‑1 (MQ=255)
                                                  ttttGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAgg                                                                   >  8:231508/1‑138 (MQ=255)
                                                      gaggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATg                                                               <  3:319514/138‑1 (MQ=255)
                                                       cggtgCTTGAATGTAATGAAGGTCTTCTTGTTGCAAGTAATCTTGATGAGCGTGTCGGCATTACCATTTATGTTATTGTTAACTCACTCTGTAAGCTGGAGAGCGCCGGTGTTATCGAGTATAGATCATTAGGAATGaa                                                             <  4:23644/135‑1 (MQ=255)
                                                       aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  5:153494/139‑1 (MQ=255)
                                                       aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  6:72203/139‑1 (MQ=255)
                                                       aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  4:1871/139‑1 (MQ=255)
                                                       aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  6:212327/139‑1 (MQ=255)
                                                        ggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaaa                                                            <  4:258705/139‑1 (MQ=255)
                                                          agCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg                                                                                            <  1:290804/105‑1 (MQ=255)
                                                          agCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg                                                                                            >  2:290804/1‑105 (MQ=255)
                                                            cTTGACGGAAATGAAGGTCTTCTTGTTGCATGTAAAATTGCTGACCGTGTCGGCATAACCAGATCTGTTATTGTGAACGCACTCAGTAAGATGGAGAGCGCCGGTGTTATCGAGTCGAGATCATTACGAATGaaacat                                                         >  8:20789/1‑135 (MQ=255)
                                                            cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTa                                                        >  5:253665/1‑139 (MQ=255)
                                                            cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTa                                                        >  8:132916/1‑139 (MQ=255)
                                                             ttGACGGTAATGAATGTCTTCTTGTTGCAAGTAAATTTGCTGACCGTGTCGGCATTAGCAGTTCTGTTTTTGTGTACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGTGTCTAGATCATTAGGAATGAAAGGTAc                                                       <  6:68123/139‑1 (MQ=255)
                                                               gACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTAc                                                       <  7:132916/137‑1 (MQ=255)
                                                                aCGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTta                                                    <  6:215946/139‑1 (MQ=255)
                                                                 cGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTtat                                                   <  3:35564/139‑1 (MQ=255)
                                                                   gAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg                                                           <  1:277545/129‑1 (MQ=255)
                                                                   gAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg                                                           >  2:277545/1‑129 (MQ=255)
                                                                     aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa                                               <  5:154285/139‑1 (MQ=255)
                                                                          aGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTa                                          <  3:73546/139‑1 (MQ=255)
                                                                                 cttGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAaac                                    >  8:175362/1‑138 (MQ=255)
                                                                                       gCAAGTAAAATTGCTGACCGTGTCGGCATTTCCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTGATCGAGTCTTGATCATTAGGAATGAAAGTTACTTATATCAAGGTACTAAACTACAAAt                             >  4:325207/1‑139 (MQ=255)
                                                                                          aGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAg                                                                            >  8:293281/1‑89 (MQ=255)
                                                                                          aGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAg                                                                            <  7:293281/89‑1 (MQ=255)
                                                                                          aGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTc                           >  3:219523/1‑138 (MQ=255)
                                                                                                       aCCGTGTAGTCATTACGAGTTCTGTTATTGTGAAAGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTTGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGa             <  4:292783/139‑1 (MQ=255)
                                                                                                        ccGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaa            <  4:219523/139‑1 (MQ=255)
                                                                                                           tgtCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             >  7:281903/1‑87 (MQ=255)
                                                                                                           tgtCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                             <  8:281903/87‑1 (MQ=255)
                                                                                                                   ttACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGACAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaaaat  >  6:283859/1‑138 (MQ=255)
                                                                                                                        |                                                                                                                                    
ATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAAT  >  BS168_reference_genome/1690638‑1690890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑