| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,364,760 | A→G | 6.2% | S111S (AGT→AGC) | qcrA ← | menaquinol:cytochrome c oxidoreductase (iron‑sulfur subunit) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,364,760 | 0 | A | G | 6.2% | 188.5 / 3.9 | 65 | S111S (AGT→AGC) | qcrA | menaquinol:cytochrome c oxidoreductase (iron‑sulfur subunit) |
| Reads supporting (aligned to +/- strand): ref base A (25/35); new base G (2/2); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GAAGCCGTCTTTTACTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAAT > BS168_reference_genome/2364628‑2364896 | gAAGCCGTCTTTTACTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGGCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTATGATACCTATTc > 6:1294/1‑139 (MQ=255) aGCCGTCTTTTACTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCa < 5:109826/139‑1 (MQ=255) aGCCGTCTTTTACTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCa < 3:330445/139‑1 (MQ=255) cGTCTTTTACATCTTGCTCATAATGATCAAGCGGCGCACGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGtt < 5:58195/139‑1 (MQ=255) gTCTTTTACTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGATTTTATTCGGGTTTTTAGGATCACTATTCCAGTTc < 5:350880/139‑1 (MQ=255) gaCTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa < 7:226290/138‑1 (MQ=255) aCTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa < 7:200131/138‑1 (MQ=255) aCTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa < 5:75802/138‑1 (MQ=255) aCTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa < 3:135008/138‑1 (MQ=255) cttcttGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTac < 3:151270/138‑1 (MQ=255) aTAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGt > 8:128028/1‑119 (MQ=255) aTAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGt < 7:128028/119‑1 (MQ=255) aTAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACAATTACAGTTAACCGTACACCCTAAATGc > 8:226290/1‑139 (MQ=255) tCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCa > 3:177935/1‑115 (MQ=255) tCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCa < 4:177935/115‑1 (MQ=255) agGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTGCGTAATGGCAGGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCAAGTTCACCGTACACACTAAATGCTTACAAATTGGc > 8:52815/1‑139 (MQ=255) agGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGc > 8:101836/1‑139 (MQ=255) agGCGAAAGAGGAGGCGTGCCAGGGACATTGGTGCAGTCCTTTTAATAGATTCCGTAATTGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTGACCGTACACCCTAAATGCATACAAATTGGc > 8:33815/1‑139 (MQ=255) cAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAg < 8:287538/139‑1 (MQ=255) cgTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTAAAAATTGGCGAAAGTGCTACAAtt < 4:18456/139‑1 (MQ=255) aTTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTa > 1:260055/1‑122 (MQ=255) aTTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTa < 2:260055/122‑1 (MQ=255) aTTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCGTACAAATTGGCGAAAGTGCTACACTTTCATACCCGgtt > 2:302595/1‑139 (MQ=255) gTGCCGTCCTTTTCCTAGAGTCCGGAATGGCCTGGACAAAAGAATTTATTCTGGTTTTTAGGCTCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTg < 3:240722/138‑1 (MQ=255) gTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTg < 7:199976/138‑1 (MQ=255) gTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTg < 8:109719/138‑1 (MQ=255) tCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa > 6:24601/1‑78 (MQ=255) tCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa < 5:24601/78‑1 (MQ=255) tCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATTCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGAcc > 8:286746/1‑138 (MQ=255) tCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGAccc > 8:24095/1‑139 (MQ=255) cTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGG‑TTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAgg < 1:279107/139‑1 (MQ=255) aTAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGAt < 3:309845/139‑1 (MQ=255) agagTCCATAATGGCATGGACAAAAGAATTTATTCGGATTTTTAGGATCGCTATTCCAGTTCACCGTCCACCCTAAATGCTTACAAATTGGCGAAAGCGCTACAATTTCATCCCCGCTTTTCTAGACCCAGGCTGAGCt > 2:346258/1‑139 (MQ=255) agTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCtt > 3:33435/1‑138 (MQ=255) gTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTGACGGTACAGCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGa < 7:33815/139‑1 (MQ=255) gTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAACTGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGa < 2:303810/139‑1 (MQ=255) gTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGa < 1:279786/139‑1 (MQ=255) gTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGa < 7:101836/139‑1 (MQ=255) gTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATATCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGa < 2:299353/139‑1 (MQ=255) cGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa < 8:111407/62‑1 (MQ=255) cGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTa > 7:111407/1‑62 (MQ=255) tAATGTCATGGACAAAAGTATTTATTCGGGTTTGTAGGATCACTATTCCAGTTCACCGTACACACTATATGCTTACAAATTGTCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGAGtctt < 4:115993/139‑1 (MQ=255) tAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGActct > 6:51943/1‑138 (MQ=255) tAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACtctt < 1:281905/139‑1 (MQ=255) cATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCt > 4:165547/1‑120 (MQ=255) cATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCt < 3:165547/120‑1 (MQ=255) cATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACt > 3:75396/1‑139 (MQ=255) cATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACt > 5:46953/1‑139 (MQ=255) cATGGACAAAAGAATTTATTCGGATTTTTAGGATCGCTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGCGCTACAATTTCATCCCCGCTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGAc > 7:283585/1‑138 (MQ=255) tGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATCCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTTACTCTTCTGACTc > 6:39587/1‑138 (MQ=255) gACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATa > 7:38436/1‑139 (MQ=255) cAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATAcc < 7:84105/139‑1 (MQ=255) cAAAAGAATTTATTCGGATTTTTAGGATCGCTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGCGCTACAATTTCATCCCCGCTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATAcc < 8:283585/139‑1 (MQ=255) aaGAATTTATTCGGGTTTTTAGGCTCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGATAGTGCTACAATTTCATCCCCGTTGTTGAAGACCCAGGCTGATCTTGACTCTTCTGGCTCGTACCAgg > 8:332792/1‑139 (MQ=255) aaGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAACTGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAgg > 4:83432/1‑139 (MQ=255) aaGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAgg > 5:149618/1‑139 (MQ=255) gAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCa < 5:1294/139‑1 (MQ=255) tttATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATc > 4:326909/1‑138 (MQ=255) aTTCGGGTTTGTAGGATCACTATTACAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGAc < 7:118160/138‑1 (MQ=255) ggTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGAtt > 2:177706/1‑139 (MQ=255) aGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAAtttt < 6:122345/138‑1 (MQ=255) ggATCGCTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGCGCTACAATTTCATCCCCGCTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGGTTAAttttg < 8:203352/138‑2 (MQ=255) tCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGa < 3:187742/95‑1 (MQ=255) tCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGa > 4:187742/1‑95 (MQ=255) tCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAAt < 4:75396/139‑1 (MQ=255) | GAAGCCGTCTTTTACTTCTTGCTCATAATGATCAAGAGGCGCAAGCGGCGGCGTGCCAGGGACATTGGTGCCGTCCTTTTCATAGAGTCCGTAATGGCATGGACAAAAGAATTTATTCGGGTTTTTAGGATCACTATTCCAGTTCACCGTACACCCTAAATGCTTACAAATTGGCGAAAGTGCTACAATTTCATCCCCGTTTTTGAAGACCCAGGCTGATCTTGACTCTTCTGACTCATACCAGGCATCGACTTGATTAATTTTAAAAT > BS168_reference_genome/2364628‑2364896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |