| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,367,214 | A→G | 9.0% | P135P (CCT→CCC) | ypiA ← | conserved protein of unknown function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,367,214 | 0 | A | G | 9.0% | 121.9 / 4.8 | 46 | P135P (CCT→CCC) | ypiA | conserved protein of unknown function |
| Reads supporting (aligned to +/- strand): ref base A (22/18); new base G (2/2); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAG > BS168_reference_genome/2367076‑2367351 | tAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCCCTTTTGCATAGGCACCCTGAGCAAAGTAAAGTTATCCCAGTTCAAAAAAAATAACa > 2:357822/1‑139 (MQ=255) ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa < 4:231643/139‑1 (MQ=255) ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa < 3:352767/139‑1 (MQ=255) ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa < 6:161264/139‑1 (MQ=255) ttCGGCCAGTCTTTGATGGACATTAACGCCTCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAAGCTCCCTGAGTAAAGTAAAGTTCCCCCAGTGCAAAATCAATAATGGGTTCa < 2:335113/139‑1 (MQ=255) ccAGTCTTTGATGGACATTCACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTc < 1:357822/139‑1 (MQ=255) cATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGc < 4:237849/139‑1 (MQ=255) aCGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTgg > 3:140102/1‑138 (MQ=255) aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTTAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGAAAAATCAATACACGGTTAATTGTCCAGAATGGACTTTGCATGATAAAGCtttgtttaa > 7:348836/1‑133 (MQ=255) aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCtt < 6:63510/132‑1 (MQ=255) aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGGTCTCCCAGTGCAATATGAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTct > 1:217095/1‑138 (MQ=255) tctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGGATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt > 6:144662/1‑139 (MQ=255) ctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAAGCTCCCTGAGTAAAGTAAAGTTCCCCCAGTGCAAAATCAATAATGGGTTCATTGTCCAGAATGGACTTTGCTTgta < 4:264731/122‑3 (MQ=255) ctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAAGCTCCCTGAGTAAAGTAAAGTTCCCCCAGTGCAAAATCAATAATGGGTTCATTGTCCAGAATGGACTTTGCTTgta > 3:264731/1‑120 (MQ=255) ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCa > 7:301427/1‑133 (MQ=255) ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCa < 8:301427/133‑1 (MQ=255) ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACt > 2:76205/1‑139 (MQ=255) ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACt > 5:330232/1‑139 (MQ=255) aGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAAt < 8:270083/139‑1 (MQ=255) ttgttTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAAccc < 5:166916/139‑1 (MQ=255) gttTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAAt < 3:268957/132‑1 (MQ=255) gttTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAAt > 4:268957/1‑132 (MQ=255) ttGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTTACTTCGAATAACCCCTGCATCTGATa > 2:320253/1‑138 (MQ=255) aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGAt < 3:364935/139‑1 (MQ=255) aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGAt < 1:76205/139‑1 (MQ=255) gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAagcagc > 5:185767/1‑138 (MQ=255) cTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACtt > 5:180776/1‑139 (MQ=255) cTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACtt > 4:232002/1‑139 (MQ=255) aGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGGCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACttt > 7:94855/1‑137 (MQ=255) cAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTc < 5:307927/136‑1 (MQ=255) cAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCg > 4:224609/1‑138 (MQ=255) cAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCACTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTc > 6:307927/1‑136 (MQ=255) aaagtaaagtTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGa < 3:224609/139‑1 (MQ=255) agtaaagtTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGACTGGAAAAGCTTTTTTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGata > 3:92814/1‑139 (MQ=255) agtaaagtTCCCCCAGTGCAAAATCAATAATGGGTTCATTGTCCAGAATGGACTTTGCTTGTAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGGTAAAGATCCGCCATAAGCAGCAAACTTTCCGGata > 8:175352/1‑139 (MQ=255) ctcCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGAt < 6:185767/138‑1 (MQ=255) tcCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGACTGGAAAAGCTTTTGTTCACTGCCTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCt > 8:275425/1‑139 (MQ=255) cccAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTg > 1:321129/1‑139 (MQ=255) ccAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGTACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCAATAAGCAGCAAACTTTCCGGATATGATGGATCTGt > 1:154400/1‑139 (MQ=255) ccAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGt > 4:119320/1‑139 (MQ=255) gTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTc < 7:152514/139‑1 (MQ=255) tGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTcc > 8:64219/1‑139 (MQ=255) gCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCg > 2:301968/1‑139 (MQ=255) gCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCg > 1:276182/1‑139 (MQ=255) aaaTCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGaa < 1:301968/139‑1 (MQ=255) aTCAATAACCGGGTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCGTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATa < 2:156290/139‑1 (MQ=255) cAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAg < 6:330232/139‑1 (MQ=255) | TAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAG > BS168_reference_genome/2367076‑2367351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |