| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,610,685 | C→T | 6.9% | V88I (GTC→ATC) | era ← | maturation of 16S RNA and assembly of 30S ribosomal subunit GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,610,685 | 0 | C | T | 6.9% | 186.0 / 3.0 | 58 | V88I (GTC→ATC) | era | maturation of 16S RNA and assembly of 30S ribosomal subunit GTPase |
| Reads supporting (aligned to +/- strand): ref base C (25/29); new base T (2/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
ATTGGTCGGGATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGCACCTTGTTTCTCGT > BS168_reference_genome/2610547‑2610820 | aTTGGTCGGTATGAATTTTATCAATTTTGTTTACGATTAAGACTACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc < 7:271207/139‑1 (MQ=255)aTTGGTCGGGATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc < 6:96021/139‑1 (MQ=255)aTTGGTCGGGATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc < 2:296909/139‑1 (MQ=255) ttGGTCGGGATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAct > 8:62187/1‑139 (MQ=255) gggATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGACTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCttt > 7:299191/1‑138 (MQ=255) gATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc > 7:260547/1‑130 (MQ=255) gATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc > 1:306850/1‑130 (MQ=255) gATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc < 2:306850/130‑1 (MQ=255) gATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc < 8:260547/130‑1 (MQ=255) tCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTgcg < 8:23329/139‑1 (MQ=255) aTTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCa > 3:52093/1‑139 (MQ=255) gATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTcatcat > 5:254617/1‑138 (MQ=255) gATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATa < 4:168548/139‑1 (MQ=255) aaTACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTccc < 2:156643/139‑1 (MQ=255) aTACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTcccc < 1:87848/139‑1 (MQ=255) aTACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTccc < 8:145433/138‑1 (MQ=255) aGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACt < 2:301250/118‑1 (MQ=255) aGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACt > 1:301250/1‑118 (MQ=255) aGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGt < 1:88843/139‑1 (MQ=255) aGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTAAGCATAGATCATAAACAAGATCAAATCGACTACTTTTAATGTATTTTGCTCAAGTTTCATCATAAAGTCCCCaact > 7:10698/1‑137 (MQ=255) tAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc < 8:143328/88‑1 (MQ=255) tAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGAc > 7:143328/1‑88 (MQ=255) tAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATg > 2:62163/1‑139 (MQ=255) tAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATg > 8:311602/1‑139 (MQ=255) tAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGAGCCACTTTCATCATAAATTCCCCAACTTTATg > 4:155157/1‑139 (MQ=255) gACATCGTCTGGAATTTCTCAATGTTGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAATATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCt < 7:47264/139‑1 (MQ=255) gACATCGTCTGCAATTTCTCAATGATGACTTCGTCGCCCGTCCCGTAGCCGTCCTCAGCAATGAGCATCAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCt < 6:168686/139‑1 (MQ=255) gACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGATTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCt > 6:81722/1‑139 (MQ=255) gACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGATTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCt > 6:75863/1‑139 (MQ=255) cGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAATATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATa > 3:244668/1‑115 (MQ=255) cGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGt > 7:275812/1‑139 (MQ=255) cGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGt > 5:36468/1‑139 (MQ=255) cGTCTGCAATTTCTCAATGATGAATTCGTAGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATa < 4:244668/115‑1 (MQ=255) cAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATg < 6:36468/138‑1 (MQ=255) atgatgAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGgtg > 2:358063/1‑139 (MQ=255) atgatgAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGgtg < 1:89837/139‑1 (MQ=255) tgaATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGgtgtgt > 7:141049/1‑138 (MQ=255) tgaATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTc < 1:235985/139‑1 (MQ=255) gtcgCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATaaaa < 7:62187/139‑1 (MQ=255) gtcgCCCTTCCCGTACCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACGTCTTTTAATGAATTTTGCGACACTTTCTTCTTAAAGTCACCAAGATTATGCTTAGGTTTATCACTCCCTGGTGTGTCtatata > 8:96657/1‑136 (MQ=255) ggcgCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATaaaa < 4:54801/137‑1 (MQ=255) ccTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGt < 8:141049/139‑1 (MQ=255) cGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGa < 1:358063/139‑1 (MQ=255) ccTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTgt > 3:346101/1‑139 (MQ=255) ccTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCACTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCttg > 7:264193/1‑137 (MQ=255) cTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTgt < 8:275812/138‑1 (MQ=255) aTTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGt < 4:346101/139‑1 (MQ=255) ttGATCATAAACAAGATCAAATCGATTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTa < 5:81722/139‑1 (MQ=255) ttGATCATAAACAAGATCAAATCGATTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTa < 5:75863/139‑1 (MQ=255) ttGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTa < 7:311602/139‑1 (MQ=255) tGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATATAGTCCCGAAGTTTTTGGTTAGGTTTATTATTCCCTGGGGTGTCATTTAAAATGGTTTGTGATGTGCCTGTCGTCTGTAc > 2:307394/1‑139 (MQ=255) tGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTAc > 5:79954/1‑139 (MQ=255) aTCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTccc > 4:44196/1‑64 (MQ=255) aTCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTccc < 3:44196/64‑1 (MQ=255) aTCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTc > 4:218241/1‑139 (MQ=255) tCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTcc < 1:62163/139‑1 (MQ=255) tAAACAATATCAAATCGACTTCTTTTAATGTATTATGCGCCACATTCATCATAAAGGCCCCAAGTTTATGCTTATGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTg < 4:28112/139‑1 (MQ=255) aaaCAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTa > 2:4978/1‑131 (MQ=255) aaaCAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTa < 1:4978/131‑1 (MQ=255) aaaCAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTg > 5:252963/1‑138 (MQ=255) aaaCAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGc > 8:276544/1‑139 (MQ=255) gATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTAc < 2:116609/126‑1 (MQ=255) gATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTAc > 1:116609/1‑126 (MQ=255) gATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGCACCTTg > 7:123481/1‑139 (MQ=255) tCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGCACCTTGTTTCtcg < 3:155157/139‑1 (MQ=255) tCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGCACCTTGTTTCtcg < 8:123481/139‑1 (MQ=255) tCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGCACCTTGTTTCtcg < 6:254617/139‑1 (MQ=255) gACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGCACCTTGTTTCtcgt < 7:276544/138‑1 (MQ=255) | ATTGGTCGGGATGAATTTTATCAATTTTGTTTACGATTAAGAATACAGGCGTAGACATCGTCTGCAATTTCTCAATGATGAATTCGTCGCCCTTCCCGTAGCCTTCCTCAGCATTGATCATAAACAAGATCAAATCGACTTCTTTTAATGTATTTTGCGCCACTTTCATCATAAAGTCCCCAAGTTTATGCTTAGGTTTATGAATCCCTGGTGTGTCAATAAAAATGGTTTGTGATGTGCCTGTCGTCAGTACTCCTTGCACCTTGTTTCTCGT > BS168_reference_genome/2610547‑2610820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |